Preclinical modeling of mechanical thrombectomy

Anagnostakou, Vania; Epshtein, Mark; Kühn, Anna Luisa; King, R; Puri, Ajit S; Gounis, Matthew J.

doi:10.1016/j.jbiomech.2021.110894

Cited by 11 publications

(14 citation statements)

References 142 publications

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“…Here, we report that the immobilization of DNA-binding compounds, through a scalable surface modification process applicable to any commercially available thrombectomy devices, can effectively improve the adherence of the occlusive clot to the thrombectomy device, while decreasing the release of secondary emboli, in a 3D phantom bench model of acute cerebral artery occlusion. 10…”

Section: Discussionmentioning

confidence: 99%

“…and aimed to evaluate the effect of surface-modified eV3 solitaire devices compared to un-modified eV3 devices (bare metal stent, BMS) in terms of clot retrieval and SE decrease. The model reproduces the conditions of a middle cerebral artery (MCA) occlusion 15 , 10 . The clot used in these experiments was prepared using thrombin-induced clotting of bovine blood and experimental clots were incubated at 37°C for 48 hours prior to use.…”

Section: Methodsmentioning

confidence: 99%

“…The model system was composed of a human vascular replica, clot model, and a physiologically relevant mock circulation flow loop. 10…”

Section: Supplementary Materialsmentioning

confidence: 99%

“…In the present study we have screened the potential of fifteen known DNA-binding compounds in terms of specific capture of extracellular DNA versus non-specific stickiness to blood components when immobilized on device-suitable alloy discs in vitro and evaluated the performances of clinical-grade, surface-modified, stent-retrievers in a simulated in vitro middle cerebral artery (MCA) occlusion model 10 .…”

Section: Introductionmentioning

confidence: 99%

“…Briefly, an AXS Catalyst 5F (Stryker, Michigan, USA) aspiration catheter connected to a Penumbra aspiration system (Alameda, California, USA) was used as an adjunctive thrombo-aspiration procedure in all experiments. The model system was composed of a human vascular replica, clot model, and a physiologically relevant mock circulation flow loop 10. 8.3.1 Vascular modelTwenty vascular replicas of the cerebral circle of Willis were built using magnetic resonance angiograms from 20 healthy patients.…”

mentioning

confidence: 99%

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Development of a clot-adhesive coating to improve the performance of thrombectomy devices

Skarbek

Anagnostakou

Procopio

et al. 2022

Preprint

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Background: The first-pass complete recanalization by mechanical thrombectomy (MT) for the treatment of stroke remains limited due to the poor integration of the clot within current devices. Aspiration can help retrieval of the main clot but fails to prevent secondary embolism in the distal arterial territory. The dense meshes of extracellular DNA, recently described in stroke-related clots, might serve as an anchoring platform for MT devices. Objective: Evaluate the potential of DNA reacting surface to aid the retention of the main clot as well as of its small fragments within the thrombectomy device and improve the potential of MT procedures. Methods: Device-suitable alloy experimental samples were coated with 15 different compounds and contacted with extracellular DNA or with human peripheral whole blood, to compare their binding to DNA versus flowing blood elements, in vitro. Clinical-grade MT devices were coated with two selected compounds and evaluated in functional bench tests aiming to studying clot retrieval and distal emboli release, concomitant with contact aspiration, using an M1 occlusion model. Results: Binding properties of samples coated with all compounds were increased for DNA (c.ca 3-fold) and decreased (c.ca 5-fold) for blood elements, essentially platelet, as compared to the bare alloy samples, in vitro. Functional testing showed that surface modification with DNA-binding compounds improved clot retrieval and significantly reduced secondary embolism during experimental recanalization of occluded artery 3D model by thrombectomy procedures. Conclusion: Our results suggest that device coating with DNA-binding compounds can considerably improve the outcome of MT procedures in stroke patients.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The model system was composed of a human vascular replica, clot model, and a physiologically relevant mock circulation flow loop. 10…”

Section: Supplementary Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%