Preclinical models for orthopedic research and bone tissue engineering

Mills, Rebecca; Bobyn, Justin D.; Little, David

doi:10.1002/jor.23824

Cited by 37 publications

(40 citation statements)

References 92 publications

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“…[ 46 ] As an alternative, a surgical osteotomy can be performed and in this technique a mid‐shaft osteotomy is made with an oscillating saw and then the periosteum is stripped around the fracture site. [ 47 ] Usually fractures present a combination of endochondral and intramembranous ossification. At the periphery of the callus, where blood perfusion is reduced, there is always a chondrogenic differentiation of the progenitor cells and a cartilage template is initially created.…”

Section: Omics Approaches To Characterize the Bone Regeneration Procementioning

confidence: 99%

Proteomic and Transcriptomic Approaches for Studying Bone Regeneration in Health and Systemically Compromised Conditions

Calciolari

Donos

2020

Proteomics Clinical Apps

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Bone regeneration is a complex biological process, where the molecular mechanisms are only partially understood. In an ageing population, where the prevalence of chronic diseases with an impact on bone metabolism is increasing, it becomes crucial to identify new strategies that would improve regenerative outcomes also in medically compromised patients. In this context, omics are demonstrating a great potential, as they offer new insights on the molecular mechanisms regulating physiologic/pathologic bone healing and, at the same time, allow the identification of new diagnostic and therapeutic targets. This review provides an overview on the current evidence on the use of transcriptomic and proteomic approaches in bone regeneration research, particularly in relation to type 1 diabetes and osteoporosis, and discusses future scenarios and potential benefits and limitations on the integration of multi-omics. It is suggested that future research will leverage the synergy of omics with statistical modeling and bioinformatics to prompt the understanding of the biology underpinning bone formation in health and medically compromised conditions. With an eye toward personalized medicine, new strategies combining the mining of large datasets and bioinformatic data with a detailed characterization of relevant phenotypes will need to be pursued to further the understanding of disease mechanisms.

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Section: Omics Approaches To Characterize the Bone Regeneration Procementioning

confidence: 99%

Proteomic and Transcriptomic Approaches for Studying Bone Regeneration in Health and Systemically Compromised Conditions

Calciolari

Donos

2020

Proteomics Clinical Apps

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“…The "ideal model" does not exist. No single animal—aside from humans—can perfectly exhibit human responses . Researchers must choose the most suitable option, considering the objectives of the study .…”

Section: Experimental Modelsmentioning

confidence: 99%

Animal modeling in bone research—Should we follow the White Rabbit?

Macedo

Feitosa

Kawamoto

et al. 2019

Anim Models and Exp Med

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In the long history of mankind, animals have played significant roles.We owe much of our intellectual sophistication and societal status to animal husbandry. 1 Farming and agriculture have allowed humans to control their environment and an improved nutrition. The first rural settlements marked the birth of the ancient civilizations, 2 but also triggered the first zoonotic outbreaks and the beginning of public health concerns. 3 The earliest records of animal use coincide with the appearance of Hippocrates' concepts and the birth of Western medicine. 2 Jumping ahead 2400 years, in the year 2018 more than 20 000 animal subjects-exceeding 200 species-were part of translational studies worldwide, 4 in pre-clinical trials. 2 Animal modelling can provide reliable data to elucidate human diseases. 5 Despite benefiting our quality of life, animal research remains controversial. 3 There is a growing sense of disapproval over using household pets in experiments. 1 New technologies have been created to replace animals in research, but the position of ideal bone substitute remains open. 6 Thus, primary research on novel models continues paramount. 7 AbstractAnimal models are live subjects applied to translational research. They provide insights into human diseases and enhance biomedical knowledge. Livestock production has favored the pace of human social development over millennia. Today's society is more aware of animal welfare than past generations. The general public has marked objections to animal research and many species are falling into disuse. The search for an ideal methodology to replace animal use is on, but animal modeling still holds great importance to human health. Bone research, in particular, has unmet requirements that in vitro technologies cannot yet fully address. In that sense, standardizing novel models remains necessary and rabbits are gaining in popularity as potential bone models. Our aim here is to provide a broad overview of animal modeling and its ethical implications, followed by a narrower focus on bone research and the role rabbits are playing in the current scenario. K E Y W O R D Sanimal experimentation, bone density, Lagomorpha, translational medical research MACEDO Et Al.

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“…Kemik iyileşme çalışmalarının çoğunda en önemli sonuç kaynamanın olması veya olmamasıdır. Son zamanlarda bazı çalışmalarda buna ek olarak kısmi kaynama durumu da tanımlanmaktadır (14). Klinikte tibia için kullanılan radyografik kaynama skalası temel alınarak, fare ve sıçanlar için bir skorlama sistemi önerilmiştir.…”

Section: Kemikdefektimodelleriunclassified

Orthopedic Models and Imaging Techniques in Preclinical Researchs

Bedel¹,

Kıraç²

2019

nts

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ÖzTendinopathies and osteoarthritis developed after cartilage damage are chronic health problems that decrease the patient's quality of life. Osteoporosis is another important health problem leading to fractures. In the treatment of osteoporotic fractures, satisfactory results cannot be achieved. In recent years, studies intended to understand the mechanisms underlying the fracture healing, and also, to provide the regeneration of cartilage and tendon have increased. Animal models are used to improve understanding of complex processes and related mechanisms in fracture healing. In cartilage and tendon pathologies, it is inevitable to use animal models in order to investigate new surgical methods and to develop and test surgical implants and materials. While tissue engineering models aim to produce new cells that will provide regeneration, orthopedic surgery models focus on implant and biomechanical research. In planning pre-clinical orthopedic studies, appropriate methods should be chosen in order to evaluate the results correctly. Histological methods are important in evaluating the microstructural properties of tissues. In addition, quantitative assessments obtained by imaging modalities provide valuable information for adaptation of the results to the clinic.

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Preclinical models for orthopedic research and bone tissue engineering

Cited by 37 publications

References 92 publications

Proteomic and Transcriptomic Approaches for Studying Bone Regeneration in Health and Systemically Compromised Conditions

Proteomic and Transcriptomic Approaches for Studying Bone Regeneration in Health and Systemically Compromised Conditions

Animal modeling in bone research—Should we follow the White Rabbit?

Orthopedic Models and Imaging Techniques in Preclinical Researchs

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