Preclinical safety assessment of photoluminescent metal quantum clusters stabilized with autologous serum proteins for host specific theranostics

Abstract

Host derived serum proteome stabilised red-emitting gold quantum clusters (or Au-QC-NanoSera or QCNS) of size range ~2 nm have been synthesised in a first reported study. The host serum was taken from bovine, murine and human origins to establish the proof of concept. In-vitro biocompatibility with normal murine L929 fibroblast cells and radiosensitisation ability against PLC/PRF/5 hepatoma cells was established. A concentration dependant radiosensitisation effect of QCNS at differential γ-radiation doses was observed with almost 90% killing of cancer cells at a radiation dose of 5Gy. Acute and subacute safety, and non-immunogenicity of autologously derived QCNS was established in in-bred C57BL/6 mice. The biodistribution analysis revealed that the QCNS were effectively cleared from the body over a course of 28 days and were found to pose no major threat to the proper functioning and morphology of the mice.

Plasmonic nanodendrites stabilized with autologous serum proteins for sustainable host specific photothermal tumor ablation

“…26 Our group has recently developed autologous approaches to utilize host derived serum proteins as stabilizers of drug nanocrystals and metal quantum clusters broadly termed 'NanoSera'. 27,28 Considering the established preclinical safety of this approach, in the present work we involved autologous whole serum proteins to form gold nanodendrites, also named Plasmonic-NanoSera (PNS). This rapid and seedless one pot synthesis allows serum proteins to serve as both a template and stabilizer of the nanodendrites.…”

Shielding against breast tumor relapse with an autologous chemo-photo-immune active Nano–Micro-Sera based fibrin implant

“…19 Furthermore, we have demonstrated the stabilization of photo-active metal nanoparticles with autologous serum proteins with confirmed safety in mouse models. 20,21 Building on previous reports, an 'all-in-one' autologous therapeutic strategy has been formulated, emphasizing the predominant utilization of host-derived biomaterials in the development of a hybrid fibrin glue. This innovative hybrid fibrin glue is investigated for its localized application, incorporating a combination of 'Nano-MicroSera' containing lapatinib, gold nanodendrites, and imiquimod.…”