Preclinical Screening Techniques for Antidiarrheal Drugs: A comprehensive review

Erhirhie, Earnest Oghenesuvwe; Emudainohwo, Tedwins Joseph Oghenebrorie; Igboeme, Sabastine Obiora; Ajaghaku, Daniel Lottana; Ujam, Treasure Nonye; Okezie, Ugochukwu Moses; Ilodigwe, Emmanuel Emeka

doi:10.5455/ajpbp.20180329014330

Cited by 7 publications

(18 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anti-diarrheal activity of 80% methanol leaf and fruit extract of L.ocymifolia was investigated with the castor oil-induced diarrheal model in mice mentioned by Umer et al 17 Thirty mice of both sexes were randomly grouped into five groups (six mice/group) and fasted overnight. 18 Mice were dosed as described in the Grouping and Dosing section. One hour after dosing, 0.5 mL of castor oil was administered to each mouse orally.…”

Section: Methodsmentioning

confidence: 99%

“…Diarrhea was graded as follows: Normal pelleted feces (0), discrete soft-formed feces (1), soft-formed feces (2), soft watery stool (3), and watery stool with little solid matter (4). 18 …”

Section: Methodsmentioning

confidence: 99%

“…where AWFC=average weight of the fecal matter of controls and AWFT=average weight of fecal matter of test groups. 18 …”

Section: Methodsmentioning

confidence: 99%

“…Thirty mice of both sexes were randomly assigned into five groups (six mice per group) and used after overnight fasting. 18 …”

Section: Methodsmentioning

confidence: 99%

“…where A=average volume or weight of intestine in the control group and B=average volume or weight of intestine in the test groups. 18 …”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Investigation of Antibacterial and Anti-Diarrhoeal Activities of 80% Methanol Leaf and Fruit Extract of Leonotis ocymifolia (Burm. F) Iwarsson (Lamiaceae)

Ayele

Abebe

Kassie

2021

JEP

View full text Add to dashboard Cite

Background Leonotis ocymifolia (Burm.F) Iwarsson (Lamiaceae) is among the medicinal plants that are claimed to have various pharmacologic activities. However the leaves and fruits of L. ocymifolia have not yet been explored scientifically for antibacterial and anti-diarrhoeal activities. This study was aimed at investigating the anti-diarrhoeal and antibacterial activities of 80% methanol leaf and fruit extract of Leonotis ocymifolia in mice and selected diarrhea causing bacterial species. Methods The leaves and fruits of Leonotis ocymifolia were extracted using 80% methanol through maceration technique. The anti-diarrheal activity was evaluated using a castor oil induced diarrheal model, prostaglandin induced anti-enteropooling, and castor oil induced charcoal meal test in mice of either sex. Data were analyzed using one-way analysis of variance followed by Tukey post-hoc test. The antibacterial activity was evaluated on using an agar well diffusion assay. Bacterial species used were Salmonella typhi , Salmonella paratyphi , Salmonella typhimurium , Shigella species , Pseudomonas aeruginosa , Staphylococcus aureus , and Escherichia coli . For anti-diarrhoeal activity, the extract was tested at 100, 200, and 400 mg/kg. Positive and negative control groups were treated with loperamide (3 mg/kg) and 2% tween 80 (10 mL/kg), respectively. Results A significant ( p <0.05) reduction in frequency of wet stools and watery content of diarrhea as well as in delaying onset of diarrhea as compared to controls was observed in mice at the stated doses. The extract showed a dose-dependent inhibition in all used models. L. ocymifolia leaf and fruit extract also showed antimicrobial activity against all tested organisms. Conclusion Results from this study collectively indicated that 80% methanol leaf and fruit extracts of L. ocymifolia possessed significant anti-diarrhoeal activity and antibacterial activities, hence provides the scientific base for its traditional use as a diarrhea treatment.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Diarrhea was graded as follows: Normal pelleted feces (0), discrete soft-formed feces (1), soft-formed feces (2), soft watery stool (3), and watery stool with little solid matter (4). 18 …”

Section: Methodsmentioning

confidence: 99%

“…where AWFC=average weight of the fecal matter of controls and AWFT=average weight of fecal matter of test groups. 18 …”

Section: Methodsmentioning

confidence: 99%

“…Thirty mice of both sexes were randomly assigned into five groups (six mice per group) and used after overnight fasting. 18 …”

Section: Methodsmentioning

confidence: 99%

“…where A=average volume or weight of intestine in the control group and B=average volume or weight of intestine in the test groups. 18 …”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Investigation of Antibacterial and Anti-Diarrhoeal Activities of 80% Methanol Leaf and Fruit Extract of Leonotis ocymifolia (Burm. F) Iwarsson (Lamiaceae)

Ayele

Abebe

Kassie

2021

JEP

View full text Add to dashboard Cite

show abstract

A pharmacologically pre-contracted smooth muscle bowel model for the study of highly-potent opioid receptor agonists and antagonists

et al. 2023

View full text Add to dashboard Cite

In vitro and in vivo evaluation of probiotic potential and safety assessment of Bacillus coagulans SKB LAB-19 (MCC 0554) in humans and animal healthcare

Chaudhari¹,

Mohan²,

Saudagar³

et al. 2022

Regulatory Toxicology and Pharmacology

View full text Add to dashboard Cite

Preclinical Screening Techniques for Antidiarrheal Drugs: A comprehensive review

Cited by 7 publications

References 0 publications

Investigation of Antibacterial and Anti-Diarrhoeal Activities of 80% Methanol Leaf and Fruit Extract of Leonotis ocymifolia (Burm. F) Iwarsson (Lamiaceae)

Investigation of Antibacterial and Anti-Diarrhoeal Activities of 80% Methanol Leaf and Fruit Extract of Leonotis ocymifolia (Burm. F) Iwarsson (Lamiaceae)

A pharmacologically pre-contracted smooth muscle bowel model for the study of highly-potent opioid receptor agonists and antagonists

In vitro and in vivo evaluation of probiotic potential and safety assessment of Bacillus coagulans SKB LAB-19 (MCC 0554) in humans and animal healthcare

Contact Info

Product

Resources

About