Precocious <i>In-Vitro</i> Flowering of Perennial Asparagus (<i>Asparagus officinalis</i> L.) Regenerants with a Chemical Inducer

Abstract

A precocious flowering system of regenerants in asparagus (Asparagus officinalis) was achieved by treatment with a chemical inducer. Somatic embryos withered completely by being processed for 8 -12 days with 200 μM n-propyl N-(3,4-dichlorophenyl)carbamate that had been dissolved in distilled water. In contrast, precocious flowering occurred at an extremely low rate (3.4%) when somatic embryos were processed in carbamate dissolved in Murashige and Skoog's liquid medium. To encapsulate the female and male embryos, we surveyed the optimum conditions of viscosity and concentration of sodium alginate for encapsulating the seeds, and we screened the values of 80 -120 cps and 2% -3%, respectively. The synthetic seeds produced also withered when they were processed with the carbamate dissolved in distilled water. However, when Murashige and Skoog's liquid medium was used for the solvent, the flowering frequency of the synthetic seeds was enhanced (13.3%). Based on our morphological and histological observations, female and male regenerants that were processed with the carbamate solution produced individual flower organs. The conversion of sex expression did not occur. A precocious flowering system would allow a significant reduction in the time required for perennial seedlings to flower and can, therefore, save time required for further experiments that employ floral homeotic mutants.

Nanoelicitors Application Promote Antioxidant Capacity of <i>Asparagus officinalis</i> (In Vitro)

“…Natural Antioxidants extracted as prominent medical secondary metabolites have been being considered by nutritionists to alter chemical synthetic antioxidants [21]. There can be found many plant tissue culture approaches employed to enhance biosynthesis of the compounds in medical plants [22] such as Asparagus officinalis L. This plant belongs to genus Asparagus that is used to cure several diseases [23]. Furthermore, A. officinalis L. is monocot and belongs to family Asparagaceae.…”

“…Inflorescences are axial and, in some cases, have single-stranded and dualbase flowers; flowers with six tepals partially fused at base; female flower with three carpels, each ovary with four seeds. [23]. A. officinalis L. has been employed as an important medical plant to remedy of several disorders and diseases for ages.…”