Preconception allergen sensitization can induce B10 cells in offspring: a potential main role for maternal IgG

Oliveira, Marília Garcia de; Oliveira, Lucas Ferrari de; Lira, Aline Aparecida de Lima; Sgnotto, Fábio da Ressureição; Duarte, Alberto José da Silva; Sato, Maria Notomi; Victor, Jefferson Russo

doi:10.1186/s13223-017-0195-8

Cited by 21 publications

(52 citation statements)

References 23 publications

(30 reference statements)

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“…Taking advantage of our previously described maternal allergen immunization protocol, which is schematically represented in Figure , we validated the inhibition of the offspring allergic response in terms of the total seric IgE, anaphylactic IgE and pulmonary allergic inflammation produced in response to OVA (Figure A,B). Maternal immunization significantly decreased the frequency of infiltrating pulmonary lymphocytes in the offspring following OVA immunization and challenge (Figure C).…”

Section: Resultsmentioning

confidence: 99%

“…IgG was purified from pooled serum according to the specifications of the Melon Gel IgG Spin Purification Kit (Thermo) and as previously described . Purified IgG was collected, sterilized and stored at −80°C for use in cell culture experiments.…”

Section: Methodsmentioning

confidence: 99%

“…Anti‐OVA antibody levels are reported as titres. The anaphylactic anti‐OVA IgE titre was measured through passive cutaneous anaphylaxis (PCA), as previously described …”

Section: Methodsmentioning

confidence: 99%

“…The cell pellet was diluted in 300 μL of PBS, and total leucocyte counts were performed using a Neubauer chamber. The lungs were surgically removed and subjected to a tissue dissociation protocol using a previously described method . Dissociated lung and BAL samples were evaluated by flow cytometry.…”

Section: Methodsmentioning

confidence: 99%

“…Regulation of allergy development in murine offspring mediated by maternal and neonatal immune status has been discussed by our group . Recent observations have highlighted the concept of the neonatal window of opportunity and the critical role of early priming for lifelong host‐microbiota and immune homoeostasis .…”

Section: Introductionmentioning

confidence: 99%

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Maternal IgG impairs the maturation of offspring intrathymic IL‐17‐producing γδT cells: Implications for murine and human allergies

de‐Oliveira

Lira

Sgnotto

et al. 2019

Clin Experimental Allergy

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Background The precise mechanism involved in the acquisition of the IL‐17+ profile of γδT cells, the ligands responsible for this change, and whether this default is acquired during intrathymic maturation need to be elucidated. Objective This study aimed to evaluate whether IL‐17‐producing γδT cells are present in the airways of tolerant offspring from allergen‐sensitized mothers and the possible implication of maternal IgG in the generation of these cells. Methods Female mice were immunized or not, and the allergic response, frequency of γδT cell subsets and cytokine production of the offspring were analysed by flow cytometry. The effects of passive in vivo transfer of purified IgG were investigated in offspring. A translational approach was employed to analyse γδT cells in the thymus and PBMCs from humans. Results Maternal immunization reduced the frequency of spontaneous IL‐17‐producing γδT cells in the thymus, spleen and lung of offspring. This effect was mimicked by the in vivo treatment of females with purified IgG. IgG directly interacted with γδT cell membranes. The modulatory effect of human IgG on human infant intrathymic and adult peripheral γδT cells showed similarities to murine γδT cells, which is rarely reported in the literature. Conclusions & Clinical Relevance Together, our results reveal that IgG from potentially tolerant atopic mothers can influence offspring thymic IL‐17‐producing γδT cell maturation. Furthermore, we suggest that IgG is an unprecedented modulatory factor of murine and human γδT cells. These observations may support the future development of IgG‐based immunoregulatory therapeutic strategies.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Anti‐OVA antibody levels are reported as titres. The anaphylactic anti‐OVA IgE titre was measured through passive cutaneous anaphylaxis (PCA), as previously described …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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