Twelve males completed three incremental, discontinuous treadmill tests in the heat [31.9(1.0)°C, 61.9(8.9)%] to determine speed at two fixed blood lactate concentrations (2 and 3.5 mmol/L), running economy (RE), and maximum oxygen uptake ( VO 2max ). Trials involved 20 min of either internal cooling (ICE, 7.5 g/kg ice slurry ingestion) or mixed-methods external cooling (EXT, cold towels, forearm immersion, ice vest, and cooling shorts), alongside no intervention (CON). Endurance exercise is underpinned by the ability to transfer chemical energy into a given exercise velocity (Coyle, 1999). The status of this biological process can be assessed using physiological markers such as the lactate thresholds, running economy (RE), and maximum oxygen uptake ( VO 2max ). Under normothermic conditions, when combined with the peak treadmill velocity, these markers have been shown to account for 97.8% of the variation in 16 km run time (McLaughlin et al., 2010). McLaughlin et al. (2010) highlighted that VO 2max accounted for 90.2% of variation in running time in a group with heterogenous VO 2max values. Furthermore, Lorenzo et al. (2011) has shown the lactate turnpoint (LTP) to be a strong predictor of time trial performance in both cold (r = 0.89) and hot (r = 0.87) environments.