PRECURSORS OF NICOTINIC ACID IN Escherichia Coli<sup>1</sup>

Ortega, Manuel V.; Brown, Gene M.

doi:10.1021/ja01525a090

Cited by 27 publications

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“…The interchangeability of aspartic acid and nicotinamide for the growth of some strains of P . pseudotuberculosis accords with the findings of Ortega & Brown (1960) and of Albertson & Moat (1965) that the biosynthesis of nicotinic acid involves condensation of aspartic acid with a 3C compound.…”

Section: Discussionsupporting

confidence: 89%

The Nutritional Requirements of some Pasteurella Species

Burrows

Gillett

1966

Journal of General Microbiology

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SUMMARYThe nutritional requirements for growth on agar media a t 28" and a t 37" were investigated for strains representative of Pmteurella pseudotuberculosis, P . pestis and Pasteurella strain ' X ' ( Yersinia enterocolitica). At 28", strains of P. pseudotuberculosis either required no growth factors or were dependent on thiamine or pantothenate. Most strains of P. pestis required cystine, methionine and phenylalanine but some strains could dispense with methionine or phenylalanine while others required additional factors.Strains of Pasteurella ' X ' either were thiamine-dependent or required no factors. At 37" all strains showed additional requirements. Most strains of P. pseudotuberculosis could then grow with any 3 of the 4 factors glutamic acid, thiamine, cystine and pantothenate ; other strains specifically required all 4 factors and nicotinamide. Strains of P. pestis grew reliably on media supplemented with cystine, methionine, phenylalanine, glycine, valine, isoleucine, glutamic acid and thiamine when incubated in C0,-enriched air, All strains of Pasteurella 'X' required thiamine and either cystine or methionine ; some substrains showed additional requirements. Virulent and avirulent representative strains of P. pestis had similar calcium requirements for growth on defined media.

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Section: Discussionsupporting

confidence: 89%

The Nutritional Requirements of some Pasteurella Species

Burrows

Gillett

1966

Journal of General Microbiology

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“…Succinate has been shown to be a precursor of the pyridone ring of ricinine in castor plants (Waller and Henderson, 1961a) and of the pyridine ring of nicotinic acid formed by E. coli (Ortega and Brown, 1959).…”

Section: Discussionmentioning

confidence: 99%

Studies on the Biosynthesis of the Pyridine Ring of Nicotine^*

Griffith¹,

Hellman²,

Byerrum³

1962

Biochemistry

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Tobacco plants were fed three C'd-labeled substrates to study their role in the biosynthesis of the pyridine ring of nicotine. Glycerol-2-C14 and aspartic a~id-3-C'~ both contributed relatively large quantities of radioactive carbon to the pyridine ring, whereas propionate-3-C14 did not. Glycerol-2-C14 was incorporated into the pyridine ring to about the same extent as glycerol-1,3-C14. Partial degradation of the pyridine ring of nicotine from plants fed aspartic acid-3-C14 revealed that aspartic acid was not converted directly to the ring, since C14 was located in more than a single position in the ring. The three labeled compounds all contributed C14 to the pyrrolidine ring of nicotine. The pattern of labeling in the pyrrolidine ring suggested that propionate was converted to acetate before utilization for nicotine biosynthesis.It has recently been shown that several metabolites which are either constituents of, or closely related to, the glycolytic pathway or the tricarboxylic acid cycle contribute carbon to some pyridine ring-containing compounds synthesized by several plants. For instance, glycer01-1,3-C'~, acetate-2-C14, and propionate-2-C14 contribute radioactive carbon to the pyridine ring of nicotine in the metabolic processes in tobacco plants (Nicotiana rustica) (Griffith et al., 1960). In addition, these same precursors along with succinate are incorporated into the 2-pyridone ring of ricinine by castor plants (Anwar et al., 1961; Waller and Henderson, 1961a, b), and Ortega and Brown (1959) have demonstrated that succinate and glycerol are utilized by Escherichia coli for the synthesis of the pyridine ring of nicotinic acid.In the studies of incorporation of various metabolites into nicotine, degradations of the alkaloid from plants fed acetate-2-C14 or propionate-2-C14 have revealed that about half of the C14 of the pyridine ring was located in carbon-3. In addition, the extent of incorporation and distribution of C14 in nicotine after feeding of labeled glycerol was different than after feeding either acetate or propionate (Griffith et al., 1960). These findings suggested that glycerol contributed carbon to one part of the pyridine ring and acetate or propionate to another part.These three precursors were incorporated into nicotine with a relatively low dilution of C14, and it appeared, therefore, that they were all closely related to the precursors which were utilized directly for pyridine ring synthesis. However, the processes leading from glycerol, acetate, and propionate to the pyridine ring are not well understood, and the purpose of the present study, therefore, was to provide further information about these processes. Evidence is presented which indicates that carbon

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“…ORTEGA u. BROWN (1959) zeigten, daR bei E . coli Glycerin-1,3-14C in Nikotinsiiure eingebaut wird, wobei die spezifische Aktivitat der gebildeten Nikotinsiiure bei gleichzeitiger Gabe von Bernsteinsiiure und Brenztraubensiiure wesentlich niedriger war.…”

Section: Da'skussionunclassified

Zinkmangel und Nikotinsäure‐Biosynthese bei Mycobacterium tuberculosis (Stamm BCG)

Mothes¹

1964

J of Basic Microbiology

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Einleitung. BCG-Bakterien sind unter bestimmten Kulturbedingungen gute Nikotinsiiurebildner. Damit diirften sie sich auch zur Kliirung der Biosynthese der Nikotinskure eignen, einer fur den Grundstoffwechsel (Co-Dehydrogenasen) wie fur den sekundiiren Stoffwechsel der Pflanzen (Alkaloide vom Typ des Nikotins und Ricinins) bedeutsamen Substanz. Die Nikotinsiiurebiosynthese stellt ein interessantes Problem der Biochemie dar, weil sie wahrscheinlich auf ganz verschiedenen Wegen verlaufen kann.Bei einigen Tieren und bei Neurospora entsteht Nikotinsiiure mit Sicherheit im Tryptophan-Abbau iiber Kynurenin-Hydroxyanthranilsiiure.

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PRECURSORS OF NICOTINIC ACID IN Escherichia Coli¹

Abstract: Communications to the Editor 4437 in comparison with the capacity for de novo synthesis, were used in this experiment.

Cited by 27 publications

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The Nutritional Requirements of some Pasteurella Species

The Nutritional Requirements of some Pasteurella Species

Studies on the Biosynthesis of the Pyridine Ring of Nicotine^*

Zinkmangel und Nikotinsäure‐Biosynthese bei Mycobacterium tuberculosis (Stamm BCG)

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PRECURSORS OF NICOTINIC ACID IN Escherichia Coli1

Abstract: Communications to the Editor 4437 in comparison with the capacity for de novo synthesis, were used in this experiment.

Cited by 27 publications

References 0 publications

The Nutritional Requirements of some Pasteurella Species

The Nutritional Requirements of some Pasteurella Species

Studies on the Biosynthesis of the Pyridine Ring of Nicotine*

Zinkmangel und Nikotinsäure‐Biosynthese bei Mycobacterium tuberculosis (Stamm BCG)

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PRECURSORS OF NICOTINIC ACID IN Escherichia Coli¹

Studies on the Biosynthesis of the Pyridine Ring of Nicotine^*