Due to their small size and high nutritional content, Moina sp. is an excellent food source for young fish such as Nile tilapia, African catfish, guppy, and carp. Moina sp. is increasingly being utilized as live feed in aquaculture, particularly in hatcheries. However, Moina sp. frequently obtained from contaminated environmental sources such as sewage with unsteady production. Thus, in order to use Moina sp. as a hygienic and sustainable source of livefeed, in this study, two culture system i.e. semi-open and open culture system, were developed and its production performance were analyzed. The highest production amount per cycle per tank for semi-open system was 733.3 ±251.7g and for the open system the highest production amount was 1463.7 ±348.2g. The mean for total biomass production in the semi-open system monthly was 6.26 ±0.92kg. Meanwhile, for the open culture system, almost five fold more production was recorded during the culture period which was 33.5 ±8.92kg. Based on the production performance of both culture system per metric tonne (MT) within the ten-month period of study, the total Moina sp. biomass produced per month per MT was almost comparable. In this study, the productions of both systems per tank per cycle in each system were consistent. The open culture system recorded significantly higher production of Moina sp. per culture cycle per tank compared to the semi-open system. The overall total productivity of each culture system every month, on the other hand, fluctuated slightly. Both culture system could be used to mass produced Moina sp. The study's findings were crucial for the prospective mass production and sustainable of Moina sp as live feed in aquaculture.