Predator Presence Alters Intestinal Microbiota in Mussel

Xie, Zhe; Xu, Guangyu; Miao, Fengze; Kong, Hui; Hu, Menghong; Wang, Youji

doi:10.1007/s00248-022-02106-5

Cited by 13 publications

(5 citation statements)

References 61 publications

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“…Based on the Kyoto Encyclopedia of Genes and Genomes prediction, the relative abundance of microbial functional abilities showed some differences between fish kairomone‐treated and control groups at any detection sites, and the differences are associated with the predation risk (Xie et al., 2022; Zha et al., 2018). In the present study, the functional abundance of the gut microbiota in the presence of fish kairomone decreased most significantly compared with that in the control.…”

Section: Discussionmentioning

confidence: 99%

“…As to whether the presence of predator affects prey-associated microbiota, first, predator kairomone can induce prey responses that fall under the category of the nerve and immune systems, which play important roles in regulating microbial communities (Candela et al, 2012;Forsythe et al, 2012), and, second, predators affect the availability of food resources to prey, while the quality and quantity of diet in the host environment are important factors that affect the host gut microbiota (Callens et al, 2016;Zha et al, 2018). Recently, Xie et al (2022) found that the most abundant genus Neisseria in the gut of Mytilus coruscus fell with the presence of the predator, while the pathogen Arcobacter increased with predator presence. Zha et al (2018) found that the abundance of Fusobacteria in the gut of Perca fluviatilis increased both at the lowest food ration and at predation stress conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Xie et al. (2022) found that the most abundant genus Neisseria in the gut of Mytilus coruscus fell with the presence of the predator, while the pathogen Arcobacter increased with predator presence. Zha et al.…”

Section: Introductionmentioning

confidence: 99%

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Fish predation risk alters the microbiota of Daphnia in the process of inducing its life‐history defence traits

Liu,

Chen,

Akbar

et al. 2024

Freshwater Biology

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Host‐associated microbiota is an essential mediator of host adaptation to changeable environmental conditions. However, whether fish predation risk alters the gut microbiota composition of Daphnia while also of inducing Daphnia's life‐history defence traits remains unclear. Examination of the effect of predation risk on prey‐associated microbiota may help in improving our understanding of induced anti‐predation defence from a microbial perspective. In this study, Daphnia magna was used as the model organism to investigate the changes in the microbiota in the gut of Daphnia as well as in the whole Daphnia and the culture medium during D. magna life‐history anti‐predation defence. The relationship of these changes with the development stage was also assessed. The results showed that predation risk can simultaneously induce anti‐predation defences and reshape the associated microbiota composition of Daphnia. The gut microbiota of D. magna responded to both developmental stages and predation risk. The Shannon diversity of gut microbiota under predation risk decreased significantly with developmental stage. The abundance of dominant bacteria such as Burkholderiaceae and Flavobacteriaceae decreased under predation risk and was further reduced with developmental stage. Functional prediction results showed that the presence of predation risk mainly weakened overall microbial function, especially metabolic function, in the gut of D. magna. The weakening of microbial function was most obvious during sexual maturity. In addition, the number of unique operational taxonomic units in the whole Daphnia and dissected guts under predation risk was significantly higher than those in control. Based on the screening results, the Pantoea genus (Erwiniaceae family) can clearly distinguish dissected gut samples with or without predation risk. The present study provided more insights into the structural features and functional roles of the Daphnia microbiota and improved our understanding of induced anti‐predation defence from a microbial perspective. Furthermore, this study highlights the possible complex correlations that exist between induced defensive traits and altered microbiota, which warrants further investigation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fish predation risk alters the microbiota of Daphnia in the process of inducing its life‐history defence traits

Liu,

Chen,

Akbar

et al. 2024

Freshwater Biology

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“…One-way analysis of variance (ANOVA) was used to analyze population differences with a significance level of P < 0.05. Using the “ggtern” and “ggplot2” packages, a ternary plot was utilized to demonstrate the relative relationship and distribution of the dominating species (>0.5% in at least one sample) between the three seasons ( Xie et al, 2022 ). We utilized SourceTracker to determine the fraction of the frog’s gut microbial community in one season that originated from other seasons.…”

Section: Methodsmentioning

confidence: 99%

Characteristics and a comparison of the gut microbiota in two frog species at the beginning and end of hibernation

Tong

Dong

et al. 2023

Front. Microbiol.

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Season has been suggested to contribute to variation in the gut microbiota of animals. The complicated relationships between amphibians and their gut microbiota and how they change throughout the year require more research. Short-term and long-term hypothermic fasting of amphibians may affect gut microbiota differently; however, these changes have not been explored. In this study, the composition and characteristics of the gut microbiota of Rana amurensis and Rana dybowskii during summer, autumn (short-term fasting) and winter (long-term fasting) were studied by high-throughput Illumina sequencing. Both frog species had higher gut microbiota alpha diversity in summer than autumn and winter, but no significant variations between autumn and spring. The summer, autumn, and spring gut microbiotas of both species differed, as did the autumn and winter microbiomes. In summer, autumn and winter, the dominant phyla in the gut microbiota of both species were Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria. All animals have 10 OTUs (>90% of all 52 frogs). Both species had 23 OTUs (>90% of all 28 frogs) in winter, accounting for 47.49 ± 3.84% and 63.17 ± 3.69% of their relative abundance, respectively. PICRUSt2 analysis showed that the predominant functions of the gut microbiota in these two Rana were focused on carbohydrate metabolism, Global and overview maps, Glycan biosynthesis metabolism, membrane transport, and replication and repair, translation. The BugBase analysis estimated that among the seasons in the R. amurensis group, Facultatively_Anaerobic, Forms_Biofilms, Gram_Negative, Gram_Positive, Potentially_Pathogenic were significantly different. However, there was no difference for R. dybowskii. The research will reveal how the gut microbiota of amphibians adapts to environmental changes during hibernation, aid in the conservation of endangered amphibians, particularly those that hibernate, and advance microbiota research by elucidating the role of microbiota under various physiological states and environmental conditions.

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“…Nevertheless, there remains a dearth of research concerning the involvement of small Mafs in the thick shell mussel M. coruscus, a crucial model organism in marine environmental studies. M. coruscus is primarily distributed in the Yellow Sea and East China Sea, adopting a lifestyle of attachment and filter feeding [21,22]. As they inhabit coastal areas prone to pollution, pollutants accumulate in various tissues, potentially leading to disrupted ROS metabolism [23].…”

Section: Introductionmentioning

confidence: 99%

The Functional Significance of McMafF_G_K in Molluscs: Implications for Nrf2-Mediated Oxidative Stress Response

Yao,

Qiu,

Zhu

et al. 2023

IJMS

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The nuclear factor erythroid 2-related factor 2 (Nrf2) is a pivotal regulator of antioxidant gene expression in mammals, forming heterodimer complexes with small Maf proteins through its BZip domain. However, the underlying mechanism of Nrf2 action in molluscs remains poorly understood. The thick shell mussel, Mytilus coruscus, represents a model organism for the marine environment and molluscs interaction research. In this study, we used in silico cloning to obtain a small Maf homologue called McMafF_G_K from M. coruscus. McMafF_G_K possesses a typical BZip domain, suggesting its affiliation with the traditional small Maf family and its potential involvement in the Nrf2 signaling pathway. Transcriptional analysis revealed that McMafF_G_K exhibited a robust response to benzo[a]pyrene (Bap) in the digestive glands. However, this response was down-regulated upon interference with McMafF_G_K-siRNA. Interestingly, the expression levels of Nrf2, NAD(P)H: quinone oxidoreductase (NQO-1), and Glutathione Peroxidase (GPx), which are key players in oxidative stress response, showed a positive correlation with McMafF_G_K in digested adenocytes of M. coruscus. Furthermore, in vitro analysis of antioxidant capacity in digestive gland cells demonstrated that Bap exposure led to an increase in reactive oxygen species (ROS) levels, accompanied by an elevation in total antioxidant capacity (T-AOC), potentially counterbalancing the excessive ROS. Strikingly, transfection of McMafF_G_K siRNA resulted in a significant rise in ROS level and a down-regulation of T-AOC level. To validate the functional relevance of McMafF_G_K, a glutathione S-transferase (GST) pull-down assay confirmed its interaction with McNrf2, providing compelling evidence of their protein interaction. This study significantly contributes to our understanding of the functional role of McMafF_G_K in the Nrf2 signaling pathway and sheds light on its potential as a target for further research in oxidative stress response.

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Predator Presence Alters Intestinal Microbiota in Mussel

Cited by 13 publications

References 61 publications

Fish predation risk alters the microbiota of Daphnia in the process of inducing its life‐history defence traits

Fish predation risk alters the microbiota of Daphnia in the process of inducing its life‐history defence traits

Characteristics and a comparison of the gut microbiota in two frog species at the beginning and end of hibernation

The Functional Significance of McMafF_G_K in Molluscs: Implications for Nrf2-Mediated Oxidative Stress Response

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