Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors

Vu, Cao-An; Chen, Wen Yih

doi:10.3390/molecules25030680

Cited by 26 publications

(17 citation statements)

References 153 publications

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“…An amplifier is indispensable for SiNWFET biosensors, helping them to eliminate interferences from intrinsic properties of the transducers and physiological environments to boost their signal-to-noise ratio [ 4 , 14 , 15 , 16 ]. Recently, aptamer has emerged as a promising candidate for this duty, since it has been demonstrated as a bio-amplifier for SiNWFET immmunosensors by stabilizing and amplifying the signal recorded from both direct and sandwich assays [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Combination of Aptamer Amplifier and Antigen-Binding Fragment Probe as a Novel Strategy to Improve Detection Limit of Silicon Nanowire Field-Effect Transistor Immunosensors

Pan

Yang

et al. 2021

Sensors

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Detecting proteins at low concentrations in high-ionic-strength conditions by silicon nanowire field-effect transistors (SiNWFETs) is severely hindered due to the weakened signal, primarily caused by screening effects. In this study, aptamer as a signal amplifier, which has already been reported by our group, is integrated into SiNWFET immunosensors employing antigen-binding fragments (Fab) as the receptors to improve its detection limit for the first time. The Fab-SiNWFET immunosensors were developed by immobilizing Fab onto Si surfaces modified with either 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA) (Fab/APTES-SiNWFETs), or mixed self-assembled monolayers (mSAMs) of polyethylene glycol (PEG) and GA (Fab/PEG-SiNWFETs), to detect the rabbit IgG at different concentrations in a high-ionic-strength environment (150 mM Bis-Tris Propane) followed by incubation with R18, an aptamer which can specifically target rabbit IgG, for signal enhancement. Empirical results revealed that the signal produced by the sensors with Fab probes was greatly enhanced compared to the ones with whole antibody (Wab) after detecting similar concentrations of rabbit IgG. The Fab/PEG-SiNWFET immunosensors exhibited an especially improved limit of detection to determine the IgG level down to 1 pg/mL, which has not been achieved by the Wab/PEG-SiNWFET immunosensors.

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Section: Introductionmentioning

confidence: 99%

Combination of Aptamer Amplifier and Antigen-Binding Fragment Probe as a Novel Strategy to Improve Detection Limit of Silicon Nanowire Field-Effect Transistor Immunosensors

Pan

Yang

et al. 2021

Sensors

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“…For example, some Field-Effect Transitor (FET)-based aptasensor was introduced. Wang et al [ 147 , 148 ] constructed an FET sensor based on aptamer and single-walled carbon nanotubes (SWNTs) to detect Pb 2+ . The double-stranded DNA containing aptamers was coupled to SWNTs.…”

Section: Aptasensors For Heavy Metals Ion Detectionmentioning

confidence: 99%

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

et al. 2021

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Heavy metal pollution has become more and more serious with industrial development and resource exploitation. Because heavy metal ions are difficult to be biodegraded, they accumulate in the human body and cause serious threat to human health. However, the conventional methods to detect heavy metal ions are more strictly to the requirements by detection equipment, sample pretreatment, experimental environment, etc. Aptasensor has the advantages of strong specificity, high sensitivity and simple preparation to detect small molecules, which provides a new direction platform in the detection of heavy metal ions. This paper reviews the selection of aptamers as target for heavy metal ions since the 21th century and aptasensors application for detection of heavy metal ions that were reported in the past five years. Firstly, the selection methods for aptamers with high specificity and high affinity are introduced. Construction methods and research progress on sensor based aptamers as recognition element are also introduced systematically. Finally, the challenges and future opportunities of aptasensors in detecting heavy metal ions are discussed.

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“…All the measurements were carried out at physiological pH (pH 7.4). The remarkable stability and sensitivity exhibited by glycan-immobilized FET biosensor over an antibody-immobilized (antibodies are the widely used recognition elements with FET) [ 110 ] one is also discussed. In addition, Goswami and co-workers developed an aptamer based extended gate FET biosensor for malaria biomarker, Plasmodium falciparum glutamate dehydrogenase (Pfgd) [ 105 ].…”

Section: Review Of Recent Electrochemical Biosensors For Eids and Re-mentioning

confidence: 99%

Recent advances and challenges in electrochemical biosensors for emerging and re-emerging infectious diseases

Menon

Mathew

Sam

et al. 2020

Journal of Electroanalytical Chemistry

148

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The rise of emerging infectious diseases (EIDs) as well as the increase in spread of existing infections is threatening global economies and human lives, with several countries still fighting repeated onslaught of a few of these epidemics. The catastrophic impact a pandemic has on humans and economy should serve as a reminder to be better prepared to the advent of known and unknown pathogens in the future. The goal of having a set of initiatives and procedures to tackle them is the need of the hour. Rapid detection and point-of-care (POC) analysis of pathogens causing these diseases is not only a problem entailing the scientific community but also raises challenges in tailoring appropriate treatment strategies to the healthcare sector. Among the various methods used to detect pathogens, Electrochemical Biosensor Technology is at the forefront in the development of POC devices. Electrochemical Biosensors stand in good stead due to their rapid response, high sensitivity and selectivity and ease of miniaturization to name a few advantages. This review explores the innovations in electrochemical biosensing based on the various electroanalytical techniques including voltammetry, impedance, amperometry and potentiometry and discusses their potential in diagnosis of emerging and re-emerging infectious diseases (Re-EIDs), which are potential pandemic threats.

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Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors

Cited by 26 publications

References 153 publications

Combination of Aptamer Amplifier and Antigen-Binding Fragment Probe as a Novel Strategy to Improve Detection Limit of Silicon Nanowire Field-Effect Transistor Immunosensors

Combination of Aptamer Amplifier and Antigen-Binding Fragment Probe as a Novel Strategy to Improve Detection Limit of Silicon Nanowire Field-Effect Transistor Immunosensors

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

Recent advances and challenges in electrochemical biosensors for emerging and re-emerging infectious diseases

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