Predicting Humification Degree of Organic Solid Waste During Aerobic Fermentation Using Designated Bacterial Community

Abstract

Background: Microbe is the driver for disposing of organic solid waste (OSW) during aerobic fermentation. Notwithstanding, the significance of microbe is always underestimated in numerous preceding studies about the aerobic fermentation products assessment. Results: Here, we investigate the humification degree (HD) and humic acid content could be assessed in terms of the bacterial community. Microbial sequencing and bioinformatics approaches are combined to analyze the biological characteristics of 105 aerobic fermentation samples collected. The bacterial communities could make predictions, which even correctly determines the categories of OSWs with 94% accuracy. Furthermore, the bacterial genera screened are designated as the bacterial code, which is substituted into the Random forest model to predict HD. And the bacteria codes can also provide a better prediction of the HD. Conclusions: Our result suggested that bacteria codes a reliable biological method is proposed to assess HD effectively. Our experiment not only proves that the aerobic fermentation can be revealed by biological means. The bacterial codes can also be used as an ecological and biological indicator to evaluate the quality of the aerobic fermentation of different materials. This study further provides new insights on the use of microbe to evaluate the content of various substances during the degradation process of OSWs.