Predicting <scp>NK</scp> cell subsets using gene expression levels in peripheral blood and endometrial biopsy specimens

Davies, Michael L.; Dambaeva, Svetlana; Katukurundage, Dimantha; Repak, Miroslava; Gilman‐Sachs, Alice; Kwak‐Kim, Joanne; Beaman, Kenneth D.

doi:10.1111/aji.12730

Cited by 5 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is still an open debate on whether, or to which extent, the peripheral NK cell subsets correlate with the endometrial NK cells in normal women, in infertile women, and in women with RPL, even though there is evidence that peripheral NK cells could reflect, at least to some degree, the local endometrial environment [131,[141][142][143][144][145][146]. This issue is beyond the scope of the present article, which is focused on the local endometrial immune environment in RPL.…”

Section: Unksmentioning

confidence: 99%

Endometrial Immune Dysfunction in Recurrent Pregnancy Loss

Ticconi

Pietropolli

Simone

et al. 2019

IJMS

159

101

View full text Add to dashboard Cite

Recurrent pregnancy loss (RPL) represents an unresolved problem for contemporary gynecology and obstetrics. In fact, it is not only a relevant complication of pregnancy, but is also a significant reproductive disorder affecting around 5% of couples desiring a child. The current knowledge on RPL is largely incomplete, since nearly 50% of RPL cases are still classified as unexplained. Emerging evidence indicates that the endometrium is a key tissue involved in the correct immunologic dialogue between the mother and the conceptus, which is a condition essential for the proper establishment and maintenance of a successful pregnancy. The immunologic events occurring at the maternal–fetal interface within the endometrium in early pregnancy are extremely complex and involve a large array of immune cells and molecules with immunoregulatory properties. A growing body of experimental studies suggests that endometrial immune dysregulation could be responsible for several, if not many, cases of RPL of unknown origin. The present article reviews the major immunologic pathways, cells, and molecular determinants involved in the endometrial dysfunction observed with specific application to RPL.

show abstract

Section: Unksmentioning

confidence: 99%

Endometrial Immune Dysfunction in Recurrent Pregnancy Loss

Ticconi

Pietropolli

Simone

et al. 2019

IJMS

159

101

View full text Add to dashboard Cite

show abstract

“…18 The pre-validated Taqman gene expression assays for CD3e (Hs01062241_m1), CD19 (Hs01047410_ StepOnePlus RT-PCR system (Life Technologies). 18 The pre-validated Taqman gene expression assays for CD3e (Hs01062241_m1), CD19 (Hs01047410_ StepOnePlus RT-PCR system (Life Technologies).…”

Section: Mrna Expression Analysismentioning

confidence: 99%

“…To analyze the expression of leukocyte-specific markers, total mRNA was isolated from endometrial samples and transcribed into cDNA as previously described. 18 The pre-validated Taqman gene expression assays for CD3e (Hs01062241_m1), CD19 (Hs01047410_ g1), CD16a (Hs04188274_m1), CD16b (Hs00275547_m1), CD57 (Hs00218629_m1), and endogenous control B2M (all from Applied Biosystems, Foster City, CA) were used to perform qRT-PCR using…”

Section: Mrna Expression Analysismentioning

confidence: 99%

Prevalence of HHV‐6 in endometrium from women with recurrent implantation failure

Coulam

Bilal

Garcia

et al. 2018

American J Rep Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…In humans, the frequencies of these populations vary between individuals, but typically lymphocytes are in the range of 70-90% [26]. The NK cell subset expressing CD16A in PBMCs is the ideal effector cell in the ADCC test [27]. The experimental results indicate that our TCR-like antibody can effectively mediate the ADCC of MCF-7 cells with 16-kDa peptide MHC.…”

Section: Discussionmentioning

confidence: 99%

Investigating the Diagnostic and Therapeutic Potential of a T Cell Receptor (TCR)-like single Domain Antibody (sDAb)-Human IgG1 Antibody against Heat Shock Protein (HSP) 16KDa/HLA-A2 for Latent Tuberculosis

Liu,

Dass,

Wong

et al. 2024

TropicalMed

View full text Add to dashboard Cite

Heat shock protein 16-kDa (HSP 16-kDa) is essential for the survival of Mycobacterium tuberculosis (M. tuberculosis) during the latent period; hence, a peptide–MHC presentation of HSP 16-kDa could be a potential diagnostic and therapeutic target for latent tuberculosis (LTB). This study aimed to generate a TCR-like single-domain antibody (sDAb)-human IgG1 antibody and subsequently investigate its diagnostic and therapeutic potential in LTB, utilizing a model cell presenting the target peptide. A previously generated TCR-like sDAB that can bind to HSP 16-kDa was first fused to a human IgG1 Fc-receptor via a linker. The fusion product, sDAb-IgG1, was expressed with HEK293-F and was subsequently purified. Its diagnostic potential was investigated via cell-based ELISA utilizing MCF-7 cells peptide-pulsed with HSP 16-kDa peptides. Investigation into the antibody-dependent cell-mediated cytotoxicity (ADCC) of MCF-7 cells was also conducted to investigate its therapeutic potential. Finally, TCR-like sDAb-IgG1 was successfully produced transiently with HEK-293F and was purified using protein A chromatography. The generated antibody was tested using cell-based ELISA, which demonstrated the effective binding of the TCR-like sDAb-IgG1 to the 16-kDa peptide–MHC on the cell surface. The ADCC assay also showed that the antibody effectively mediated the ADCC of MCF-7 cells with the help of 16-kDa peptide–MHC. This allows us to hypothesize the possible utility of the said antibody for both diagnostics and therapeutics of latent tuberculosis after more investigations with clinical samples.

show abstract

Predicting NK cell subsets using gene expression levels in peripheral blood and endometrial biopsy specimens

Abstract: Strong correlations between RT-qPCR data and levels of lymphocyte subsets indicate that gene expression analysis will be a useful technique for characterizing levels of CD56+ cells in endometrial tissues.

Cited by 5 publications

References 36 publications

Endometrial Immune Dysfunction in Recurrent Pregnancy Loss

Endometrial Immune Dysfunction in Recurrent Pregnancy Loss

Prevalence of HHV‐6 in endometrium from women with recurrent implantation failure

Investigating the Diagnostic and Therapeutic Potential of a T Cell Receptor (TCR)-like single Domain Antibody (sDAb)-Human IgG1 Antibody against Heat Shock Protein (HSP) 16KDa/HLA-A2 for Latent Tuberculosis

Contact Info

Product

Resources

About