Predicting the chemical composition and structure of Aspergillus nidulans hyphal wall surface by atomic force microscopy

Abstract

In fungi, cell wall plays an important role in growth and development. Major macromolecular constituents of the aspergilli cell wall are glucan, chitin, and protein. We examined the chemical composition and structure of the Aspergillus nidulans hyphal wall surface by an atomic force microscope (AFM). To determine the composition of the cell wall surface, the adhesion forces of commercially available beta-glucan, chitin, and various proteins were compared to those of corresponding fractions prepared from the hyphal wall. In both setups, the adhesion forces of beta-glucan, chitin, and protein were 25-50, 1000-3000, and 125-300 nN, respectively. Adhesion force analysis demonstrated that the cell surface of the apical tip region might contain primarily chitin and beta-glucan and relatively a little protein. This analysis also showed the chemical composition of the hyphal surface of the mid-region would be different from that of the apical region. Morphological images obtained by the tapping mode of AFM revealed that the hyphal tip surface has moderate roughness.

Engineering Nanogels for Drug Delivery to Pathogenic Fungi Aspergillus fumigatus by Tuning Polymer Amphiphilicity

“…Together with chitin, they provide mechanical stability of the cell wall. 33 As shown by Chavan et al, 12 these polysaccharides are responsible for the absorption of PEGylated liposomes on the hyphae of A. fumigatus. Because of the similarities of PEG and PG, a similar outcome was hypothesized, and therefore, the interaction of NGs with β-glucans and fungal hyphae was studied.…”

“…β-Glucans are polysaccharides in the cell wall of fungal and yeast species. Together with chitin, they provide mechanical stability of the cell wall . As shown by Chavan et al, these polysaccharides are responsible for the absorption of PEGylated liposomes on the hyphae of A.…”