“…We simulated a series of pool numbers to evaluate the absolute bias and signal-to-noise ratio across different numbers of pools; our results indicated that greater numbers of bulked pools improve the mapping resolution of BSA (Supplemental Figure 5). As previously reported, more bulked individuals increases recombination events in BSA (Shen and Messer, 2022). Our statistical results confirmed that better dissection could be obtained using multiple pools (Supplemental Figures 2-5).…”