Predicting the orientation of magnetic microgel rods for soft anisotropic biomimetic hydrogels

Rose, Jonas C.; Fölster, Maaike; Kivilip, Lukas; Gerardo‐Nava, José; Jaekel, Esther E.; Gehlen, David B.; Rohlfs, Wilko; Laporte, Laura De

doi:10.1039/c9py01008d

Cited by 32 publications

(48 citation statements)

References 46 publications

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“…In fact, cell-laden hydrogel microfibers have been used as an excellent tissue building block ( 15,16 ). Also the ability of elongated microgels ( 17 ) has been shown to be useful in guidance of neurons ( 18 ). Recently micro-ribbons produced by wet spinning and further processed into smaller parts showed the possible advantages of anisotropic gels in cartilage engineering ( 19 ).…”

Section: Introductionmentioning

confidence: 99%

3D Bioprinting of Architected Hydrogels from Entangled Microstrands

Kessel¹,

Lee²,

Bonato³

et al. 2019

Preprint

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31Hydrogels are an excellent biomimetic of the extracellular matrix and have found great 32 use in tissue engineering. Nanoporous monolithic hydrogels have limited mass transport, 33 restricting diffusion of key biomolecules. Structured microbead-hydrogels overcome some 34 of these limitations, but suffer from lack of controlled anisotropy. Here we introduce a 35 novel method for producing architected hydrogels based on entanglement of microstrands. 36 The microstrands are mouldable and form a porous structure which is stable in water. 37 Entangled microstrands are useable as bioinks for 3D bioprinting, where they align during 38 the extrusion process. Cells co-printed with the microstrands show excellent viability and 39 augmented matrix deposition resulting in a modulus increase from 2.7 kPa to 780.2 kPa 40 after 6 weeks of culture. Entangled microstands are a new class of bioinks with 41 unprecedented advantages in terms of scalability, material versatility, mass transport, 42 showing foremost outstanding properties as a bioink for 3D printed tissue grafts. 43 44 45 46 47 48 49 50 129 130 131 Results 132 133 Entangled Microstrand Materials are Mouldable, Stable in Water and Macroporous 134 135Here we report on a robust and versatile method for preparing 'entangled' microstrands. 136 Bulk hyaluronan-methacrylate (HA-MA) hydrogels were mechanically pressed through a 137

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Section: Introductionmentioning

confidence: 99%

3D Bioprinting of Architected Hydrogels from Entangled Microstrands

Kessel¹,

Lee²,

Bonato³

et al. 2019

Preprint

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“…Therefore, the hydrogel has to be modified with biodegradable groups like esters that can be broken by enzymes secreted by the cells of interest, for example matrix metalloproteases. Another problem of most hydrogels is the orientation of the structure inside the hydrogel, because many tissues have an ordered structure and provide cells a direction to grow and migrate (Rose et al, 2020). In addition, natural and synthetic hydrogels are often subject to numerous modification steps and therefore not very cost efficient.…”

Section: Hydrogels As Matrices For Cell Cultivationmentioning

confidence: 99%

Hydrogels Derivatized With Cationic Moieties or Functional Peptides as Efficient Supports for Neural Stem Cells

et al. 2020

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The increasing incidence of neurodegenerative diseases such as Alzheimer's or Parkinson's disease represents a significant burden for patients and national health systems. The conditions are primarily caused by the death of neurons and other neural cell types. One important aim of current stem cell research is to find a way to replace the lost cells. In this perspective, neural stem cells (NSCs) have been considered as a promising tool in the field of regenerative medicine. The behavior of NSCs is modulated by environmental influences, for example hormones, growth factors, cytokines, and extracellular matrix molecules or biomechanics. These factors can be studied by using well-defined hydrogels, which are polymeric networks of synthetic or natural origin with the ability to swell in water. These gels can be modified with a variety of molecules and optimized with regard to their mechanical properties to mimic the natural extracellular environment. In particular modifications applying distinct units such as functional domains and peptides can modulate the development of NSCs with regard to proliferation, differentiation and migration. One well-known peptide sequence that affects the behavior of NSCs is the integrin recognition sequence RGD that has originally been derived from fibronectin. In the present review we provide an overview concerning the applications of modified hydrogels with an emphasis on synthetic hydrogels based on poly(acrylamides), as modified with either cationic moieties or the peptide sequence RGD. This knowledge might be used in tissue engineering and regenerative medicine for the therapy of spinal cord injuries, neurodegenerative diseases and traumata.

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“…[ 92 ] C) In vivo demonstration of nerve cell orientation along magnetically aligned anisotropic microgels. [ 93 ] D) Computer simulation of osteosarcoma cell alignment in 2% w/v sodium alginate using an acoustic frequency of 2 MHz. [ 94 ] Viability staining (green) showing 90% of viable cells both in unaligned and aligned samples.…”

Section: Digital Fabrication and Computational Augmented Modeling Of mentioning

confidence: 99%

“…For instance, superparamagnetic nanoparticles were used to induce the alignment of anisometric microgels both in vitro and inside tissue for neural cell orientation ( Figure 5B,C). [92,93] The alignment of the microgels was simulated in silico to support future in vivo experiments ( Figure 5B). [93] Another study focused on using ultrasound acoustic waves to create uneven pressure fields that can pattern cells into well-defined geometries (Figure 5D).…”

Section: Design Of Macroscopic and Microscopic Input Geometries And Cmentioning

confidence: 99%

“…[92,93] The alignment of the microgels was simulated in silico to support future in vivo experiments ( Figure 5B). [93] Another study focused on using ultrasound acoustic waves to create uneven pressure fields that can pattern cells into well-defined geometries (Figure 5D). [94] This acoustophoretic method enabled label-free, i.e., without chemical (chemotaxis) or magnetic (magnetophoresis) labels, patterning of cells encapsulated in 2% w/v alginate hydrogel but the cells did not yet show elongated morphologies after culture.…”

Section: Design Of Macroscopic and Microscopic Input Geometries And Cmentioning

confidence: 99%

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Digitally Fabricated and Naturally Augmented In Vitro Tissues

Campos

Laporte

2020

Adv Healthcare Materials

Self Cite

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Human in vitro tissues are extracorporeal 3D cultures of human cells embedded in biomaterials, commonly hydrogels, which recapitulate the heterogeneous, multiscale, and architectural environment of the human body. Contemporary strategies used in 3D tissue and organ engineering integrate the use of automated digital manufacturing methods, such as 3D printing, bioprinting, and biofabrication. Human tissues and organs, and their intra‐ and interphysiological interplay, are particularly intricate. For this reason, attentiveness is rising to intersect materials science, medicine, and biology with arts and informatics. This report presents advances in computational modeling of bioink polymerization and its compatibility with bioprinting, the use of digital design and fabrication in the development of fluidic culture devices, and the employment of generative algorithms for modeling the natural and biological augmentation of in vitro tissues. As a future direction, the use of serially linked in vitro tissues as human body‐mimicking systems and their application in drug pharmacokinetics and metabolism, disease modeling, and diagnostics are discussed.

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Predicting the orientation of magnetic microgel rods for soft anisotropic biomimetic hydrogels

Abstract: Predicting the orientation rate of rod-shaped magnetic microgels to induce unidirectional regeneration of complex and sensitive tissues.

Cited by 32 publications

References 46 publications

3D Bioprinting of Architected Hydrogels from Entangled Microstrands

3D Bioprinting of Architected Hydrogels from Entangled Microstrands

Hydrogels Derivatized With Cationic Moieties or Functional Peptides as Efficient Supports for Neural Stem Cells

Digitally Fabricated and Naturally Augmented In Vitro Tissues

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