Water temperature plays a crucial role in water ecological environment both in the reservoir and downstream area. Three Gorges Project (TGP) is the largest hydraulic engineering in the world, and changes of water quality attract much more attention, especially in the thermal structure since impoundment. In order to clearly understand water temperature distribution after impoundment in the Three Gorges Reservoir (TGR), we monitored the temperature distribution of the Taipingxi section which was not far from the dam from early April to the end of July. According to the analyzing of the monitoring data of transverse and vertical temperature variation, we could find that the temperature mixed uniformly in transverse direction. Excepting April, there was basically had no temperature difference in vertical direction, even in April, the maximum temperature difference was only 1.39°C within 100 meters (April 22), the average temperature gradient was only 0.014°C/m; Both the temperature of water and atmosphere have similar variation tendency, but the response of the water temperature to atmosphere is delayed, especially the bottom water temperature. The result indicate that the reservoir has mixed thermal structure during impoundment phase, so the release temperature had little change after the impoundment, the work provide a scientific basis for the development of pollution control and ecological protection measure.