Prediction and evaluation of the effect of pre-centrifugation sample management on the measurable untargeted LC-MS plasma metabolome

Zheng, Rui; Brunius, Carl; Shi, Lin; Zafar, Huma; Paulson, Linda; Landberg, Rikard; Naluai, Åsa Torinsson

doi:10.1016/j.aca.2021.338968

Cited by 14 publications

(16 citation statements)

References 42 publications

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“…The luminal factors of fecal supernatants were analyzed at Chalmers Mass Spectrometry Infrastructure using a non‐targeted liquid chromatography–mass spectrometry (LC‐MS) approach. Briefly, the analyses were carried out using a reversed‐phase chromatography (RP) and hydrophilic interaction chromatography (HILIC) with positive and negative electrospray ionization polarities, as described elsewhere 29 …”

Section: Methodsmentioning

confidence: 99%

“…Briefly, the analyses were carried out using a reversed-phase chromatography (RP) and hydrophilic interaction chromatography (HILIC) with positive and negative electrospray ionization polarities, as described elsewhere. 29 The samples of each study group were analyzed in separate batches, which included its own quality control samples.…”

Section: Untargeted Liquid Chromatography-mass Spectrometry Analysismentioning

confidence: 99%

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Fecal luminal factors from patients with irritable bowel syndrome induce distinct gene expression of colonoids

Iribarren

Nordlander

Sundin

et al. 2022

Neurogastroenterology Motil

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Background: Alteration of the host-microbiota cross talk at the intestinal barrier may participate in the pathophysiology of irritable bowel syndrome (IBS). Therefore, we aimed to determine effects of fecal luminal factors from IBS patients on the colonic epithelium using colonoids.Methods: Colon-derived organoid monolayers, colonoids, generated from a healthy subject, underwent stimulation with fecal supernatants from healthy subjects and IBS patients with predominant diarrhea, phosphate-buffered saline (PBS), or lipopolysaccharide (LPS). Cytokines in cell cultures and fecal LPS were measured by ELISA and mRNA gene expression of monolayers was analyzed using Qiagen RT 2 Profiler PCR Arrays. The fecal microbiota profile was determined by the GA-map ™ dysbiosis test and the fecal metabolite profile was analyzed by untargeted liquid chromatography/ mass spectrometry. Key results:Colonoid monolayers stimulated with fecal supernatants from healthy subjects (n = 7), PBS (n = 4) or LPS (n = 3) presented distinct gene expression profiles, with some overlap (R 2 Y = 0.70, Q 2 = 0.43). Addition of fecal supernatants from healthy subjects and IBS patients (n = 9) gave rise to different gene expression profiles of the colonoid monolayers (R 2 Y = 0.79, Q 2 = 0.64). Genes (n = 22) related to immune response (CD1D, TLR5) and barrier integrity (CLDN15, DSC2) contributed to the separation. Levels of proinflammatory cytokines in colonoid monolayer cultures were comparable when stimulated with fecal supernatants from either donor types.Fecal microbiota and metabolite profiles, but not LPS content, differed between the study groups.

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Section: Methodsmentioning

confidence: 99%

Section: Untargeted Liquid Chromatography-mass Spectrometry Analysismentioning

confidence: 99%

Fecal luminal factors from patients with irritable bowel syndrome induce distinct gene expression of colonoids

Iribarren

Nordlander

Sundin

et al. 2022

Neurogastroenterology Motil

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“…Another limitation is the blood sampling procedure, as blood samples were sent via overnight mail before being processed. Even if this may have in uenced the absolute SCFA concentration [50], it is unlikely to have affected group differences since all samples were collected and handled in this same way. We did in fact detect plasma concentrations of butyrate higher than previously observed in any dataset analyzed in the laboratory (data not shown), while formate was somewhat lower than typically observed [51].…”

Section: Discussionmentioning

confidence: 99%

Plasma concentrations of short-chain fatty acids in active and recovered anorexia nervosa

Nilsson

Xu²,

Landberg³

et al. 2022

Preprint

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Anorexia nervosa (AN) is one of the most lethal psychiatric disorders, and to date, we lack adequate knowledge about the (neuro)biological mechanisms of the disorder to inform evidence-based pharmacological treatment. Gut dysbiosis is a trending topic in mental health, including AN. Communication between the gut microbiota and the brain is partly mediated by metabolites produced by the gut microbiota such as short-chain fatty acids (SCFA). Previous research has suggested a role of SCFA in weight regulation, e.g., correlations between specific SCFA-producing bacteria and BMI have been demonstrated. Moreover, fecal SCFA concentrations are reported to be altered in active AN. However, data on SCFA concentrations in individuals who have recovered from AN are limited. In the present study, we analyzed and compared the plasma concentrations of seven SCFA (acetic-, butyric-, formic-, isobutyric-, isovaleric-, propionic- and succinic acid) in females with active AN (N = 109), recovered from AN (AN-REC, N = 108), and healthy-weight age-matched controls (CTRL, N = 110), and explored correlations between SCFA concentrations and BMI. Significantly lower plasma concentrations of butyric, isobutyric-, and isovaleric acid were detected in AN as well as AN-REC compared with CTRL. We also show significant correlations between plasma concentrations of SCFA and BMI. These results encourage studies evaluating whether interventions directed toward altering gut microbiota and SCFA could support weight restoration in AN.

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“…Another limitation is the blood sampling procedure, as blood samples were sent via overnight mail before being processed. Even if this may have influenced the absolute SCFA concentration [61], it is unlikely to have affected group differences since all samples were collected and handled in this same way. We did in fact detect plasma concentrations of butyrate higher than previously observed in any dataset analyzed in the laboratory (data not shown), while formate was somewhat lower than typically observed [62].…”

Section: Discussionmentioning

confidence: 99%

Plasma Concentrations of Short-Chain Fatty Acids in Active and Recovered Anorexia Nervosa

Landberg

Lavebratt

et al. 2022

Nutrients

Self Cite

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Anorexia nervosa (AN) is one of the most lethal psychiatric disorders. To date, we lack adequate knowledge about the (neuro)biological mechanisms of this disorder to inform evidence-based pharmacological treatment. Gut dysbiosis is a trending topic in mental health, including AN. Communication between the gut microbiota and the brain is partly mediated by metabolites produced by the gut microbiota such as short-chain fatty acids (SCFA). Previous research has suggested a role of SCFA in weight regulation (e.g., correlations between specific SCFA-producing bacteria and BMI have been demonstrated). Moreover, fecal SCFA concentrations are reported to be altered in active AN. However, data concerning SCFA concentrations in individuals who have recovered from AN are limited. In the present study, we analyzed and compared the plasma concentrations of seven SCFA (acetic-, butyric-, formic-, isobutyric-, isovaleric-, propionic-, and succinic acid) in females with active AN (n = 109), recovered from AN (AN-REC, n = 108), and healthy-weight age-matched controls (CTRL, n = 110), and explored correlations between SCFA concentrations and BMI. Significantly lower plasma concentrations of butyric, isobutyric-, and isovaleric acid were detected in AN as well as AN-REC compared with CTRL. We also show significant correlations between plasma concentrations of SCFA and BMI. These results encourage studies evaluating whether interventions directed toward altering gut microbiota and SCFA could support weight restoration in AN.

show abstract

Prediction and evaluation of the effect of pre-centrifugation sample management on the measurable untargeted LC-MS plasma metabolome

Cited by 14 publications

References 42 publications

Fecal luminal factors from patients with irritable bowel syndrome induce distinct gene expression of colonoids

Fecal luminal factors from patients with irritable bowel syndrome induce distinct gene expression of colonoids

Plasma concentrations of short-chain fatty acids in active and recovered anorexia nervosa

Plasma Concentrations of Short-Chain Fatty Acids in Active and Recovered Anorexia Nervosa

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