Prediction and Modulation of Platelet Recovery by Discontinuous Centrifugation of Whole Blood for the Preparation of Pure Platelet-Rich Plasma

Perez, Amanda Gomes Marcelino; Lichy, Rafael; Lana, José Fábio Santos Duarte; Rodrigues, Ana Amélia; Luzo, Ângela Cristina Malheiros; Belangero, William Dias; Santana, Maria Helena Andrade

doi:10.1089/biores.2013.0015

Cited by 27 publications

(39 citation statements)

References 19 publications

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“…In our study, PRP was prepared by a single centrifuge method where the blood cells were manually separated into layers and the kit was not used. In our PRP preparation protocol that supports the study of Perez et al (15), optimum platelet concentration was achieved with low centrifugation time at high acceleration force. Thus, the proliferation of dermal papillary cells is increased and apoptosis is prevented by effectively prepared PRP.…”

Section: Discussionsupporting

confidence: 80%

Clinical Results of Platelet-Rich Plasma Injection in the Treatment of Androgenetic Alopecia

Özer¹,

Çolak²

2019

eamr

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Platelet-rich plasma (PRP) represents a small volume of plasma in which the platelet concentration in normal blood is increased to optimum values. Its effect depends on the growth factors released from platelets. Although it has been investigated and used in many areas of plastic surgery, PRP injection has recently attracted interest in the treatment of hair loss. In this study, we aimed to evaluate the efficacy of adjuvant therapy in patients with male and female alopecia by establishing a standard protocol for the preparation and application of PRP. Methods: Fifteen patients with mild to moderate androgenetic alopecia (Ludwig alopecia score I-II for women and Hamilton-Norwood score 1-4 for men) who had not received topical or systemic treatment for alopecia in the last 6 months were included in the study. Eleven of the patients were male and 4 were female. The mean age was 41 years (27-55). PRP was prepared using a single spin method with high acceleration force and low centrifugation time, and three sessions were performed with an interval of 3-4 weeks. Patient satisfaction was assessed by visual analogue scale (VAS). The assessments on day 0, 1 st month, 2 nd month and 6 th month were evaluated retrospectively. Results: Hair pull test was positive in all patients (100%) before the treatment and the mean number of hair loss was 6.7. After the third treatment session, the hair pull test was negative in 13 patients (86.6%) with a mean of number of 2.5 hair loss. An improvement in hair density and quality was demonstrated with macroscopic photographs taken in a standard manner before and after the procedure. The mean VAS value decreased from 6.8 to 2.1 after the procedure. Discussion: PRP injections, which are prepared in a simple way without using a commercial kit, are an effective treatment option in alopecia treatment with high patient satisfaction and low cost.

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Section: Discussionsupporting

confidence: 80%

Clinical Results of Platelet-Rich Plasma Injection in the Treatment of Androgenetic Alopecia

Özer¹,

Çolak²

2019

eamr

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“…PRP Concentration . P-PRP (plasma rich in platelets and poor in leukocytes) was prepared according to Perez et al [ 50 ]. Briefly, whole blood (WB) was collected into 3.5 mL vacuum tubes (Vacuette, Campinas, SP, Brazil) containing sodium citrate 3.2% (w/v) as an anticoagulant.…”

Section: Methodsmentioning

confidence: 99%

Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine

Shimojo

Perez

Galdames

et al. 2015

The Scientific World Journal

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This study aimed to evaluate the in vitro performance of activated platelet-rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. The sponges were prepared by controlled freezing (−20, −80, or −196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v). The platelet-rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum. The composite scaffolds were prepared by embedding the sponges with the activated platelet-rich plasma. The results showed the performance of the scaffolds was superior to that of activated platelet-rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells. The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a −20°C freezing temperature, while −196°C favored osteogenic differentiation. Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.

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“…New methods of analyzing centrifugation forces on platelets, RBCs, and WBCs and the use of discontinuous centrifugation allow for the separation of PRP of varying compositions, such as pure PRP without RBCs and white blood cells [339]. Some methods can yield relatively consistent PRP compositions, such as a double centrifugation tubes allow the consistent separation of dog PRP at about four times the base level and WBCs at not more than twofold above base level [340].…”

Section: Cautions In Using Prp: Prp Compositionmentioning

confidence: 99%

“…Further studies are attempting to determine how to obtain a constant PRP composition with the ideal concentration of platelets and whether there are benefits to include or exclude RBCs and WBCs [339]. Accomplishing this may require the use of a double centrifugation approach, which allows a reduction in the WBC concentration [340].…”

Section: Cautions In Using Prp: Prp Compositionmentioning

confidence: 99%

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Kuffler

2015

Mol Neurobiol

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Platelet-rich plasma (PRP) has been tested in vitro, in animal models, and clinically for its efficacy in enhancing the rate of wound healing, reducing pain associated with injuries, and promoting axon regeneration. Although extensive data indicate that PRP-released factors induce these effects, the claims are often weakened because many studies were not rigorous or controlled, the data were limited, and other studies yielded contrary results. Critical to assessing whether PRP is effective are the large number of variables in these studies, including the method of PRP preparation, which influences the composition of PRP; type of application; type of wounds; target tissues; and diverse animal models and clinical studies. All these variables raise the question of whether one can anticipate consistent influences and raise the possibility that most of the results are correct under the circumstances where PRP was tested. This review examines evidence on the potential influences of PRP and whether PRP-released factors could induce the reported influences and concludes that the preponderance of evidence suggests that PRP has the capacity to induce all the claimed influences, although this position cannot be definitively argued. Well-defined and rigorously controlled studies of the potential influences of PRP are required in which PRP is isolated and applied using consistent techniques, protocols, and models. Finally, it is concluded that, because of the purported benefits of PRP administration and the lack of adverse events, further animal and clinical studies should be performed to explore the potential influences of PRP.

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Prediction and Modulation of Platelet Recovery by Discontinuous Centrifugation of Whole Blood for the Preparation of Pure Platelet-Rich Plasma

Cited by 27 publications

References 19 publications

Clinical Results of Platelet-Rich Plasma Injection in the Treatment of Androgenetic Alopecia

Clinical Results of Platelet-Rich Plasma Injection in the Treatment of Androgenetic Alopecia

Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

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