Prediction and optimization of gene transfection and drug delivery by electroporation

Gene delivery to human bone marrow mesenchymal stem cells by microporation

“…The usefulness of a statistical approach to quantitatively predict molecular uptake and cell viability following conventional electroporation was previously reported (Canatella and Prausnitz, 2001). In our study, a face-centered composite design methodology has allowed to evaluate the effect of electric pulses, pulse width and DNA amount on the efficacy of gene delivery by microporation using a buffer of known composition.…”

Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode

“…These data agree with expression optimization studies performed in other tissues. [17][18][19][20] A model based on previously collected data 21 analyzes viability (V) as well as number of molecules taken up (N) after electroporation delivery. For multiple pulses, the model predicts that at field strengths of o1000 V/cm, the NV increases as pulse length increases.…”

An electroporation microchip system for the transfection of zebrafish embryos using quantum dots and GFP genes for evaluation

“…In addition, it can be used to deliver specific extra-cellular genes into targets within a very short time. Nowadays, the EP method is used in cancer research (Canatella and Prausnitz 2001;Jamieson et al 1989;Nishi et al 1996;Yang and Sun 1995), gene packaged with lipids, peptides and viruses transfection (Coulberson et al 2003;Tryfona and Bustard 2006), and drug delivery (Canatella and Prausnitz 2001). In addition, the EP method is successful in transgenic treatments in mice (Lavitrano et al 1989;Marti et al 2004;Neumann et al 1982;Nishi et al 1996), silkworm (Shamila and Mathavan 1998), sea urchin (Larochelle and Epel 1991), cattle (Gagne et al 1991), chick (Nakamura andFunahashi 2001), abalone (Powers et al 1995), and fish (Rambabu et al 2005;Connaughton et al 2004).…”