Prediction and verification of microRNAs related to proline accumulation under drought stress in potato

Yang, Jie; Zhang, Ning; Ma, Congyu; Qu, Yun; Si, Huaijun; Wang, Di

doi:10.1016/j.compbiolchem.2013.04.006

Cited by 36 publications

(21 citation statements)

References 41 publications

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“…Proline is involved in the stabilization of proteins and membranes, serves as an intermediary nitrogen store and also acts as a source of carbon and energy during cell rehydration (Maggio et al, 2002;Verbruggen & Hermans, 2008). In potato, several other studies also detected an accumulation of proline in leaves during drought stress (Farhad et al, 2011;Levy, 1983;Yang et al, 2013). In a study with six potato varieties, proline increased earlier in drought-susceptible than in drought-tolerant varieties .…”

mentioning

confidence: 97%

Drought stress‐induced changes in starch yield and physiological traits in potato

Rudack

Seddig

Sprenger

et al. 2017

J Agronomy Crop Science

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Drought stress is an important limitation for potato (Solanum tuberosum L.) production as potato depends on appropriate water availability for high yields of good quality. Therefore, especially in the background of climate change, it is an important goal in potato breeding to improve drought stress tolerance. In this study, 34 European starch potato cultivars were evaluated for drought stress tolerance by growing under well‐watered and long‐term drought stress conditions in rainout shelters in 2 years’ pot trials. Besides yield, six physiological traits, that is free proline content, osmolality, total soluble sugar content, chlorophyll content (SPAD), cell membrane stability and crude protein content, were determined in leaves sampled during vegetative growth and during flowering to investigate their association with drought tolerance. ANOVA revealed significant treatment effects for all physiological traits and increased genotypic effects at flowering. The sensitivity of physiological traits to drought was significantly higher during flowering than during vegetative growth. Drought stress decreased starch yield significantly (p < .001), on average by 55%. Starch yield was significantly influenced by genotype and genotype × treatment interactions. Stress tolerance index (STI) calculated from starch yield ranged from 0.26 (sensitive) to 0.76 (tolerant) with significant genotype effects (p ≤ .001). STI correlated positively with cell membrane stability (r = .59) and crude protein content (r = .38) and negatively with osmolality (r = −.57) and total soluble sugar content (r = −.71). These contrary correlations suggest a dual adaptation strategy in potato under long‐term drought stress conditions including increased membrane stability combined with an increased osmolality due to an increased soluble sugar content.

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mentioning

confidence: 97%

Drought stress‐induced changes in starch yield and physiological traits in potato

Rudack

Seddig

Sprenger

et al. 2017

J Agronomy Crop Science

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“…Another major criterion for determining the stability is the lower the MFE value more stable the RNA secondary structure (Bonnet et al, 2004). The MFE of the identified potato pre-miRNAs in this study was varied from -55.2 (stu-miR2673) to -338 (stu-miR395x) kcal mol −1 however, this large variation in MFE values signifies variation in their sequence length (Yang et al, 2013).…”

Section: Identification Of Mirnasmentioning

confidence: 99%

“…In another study, miR1510 family, a legume-specific family, was detected in tissue-specific organ profiles by massive-scale RNA sequencing in common bean (Peláez et al, 2012). Fifth, miR2673 family has been identified for proline accumulation under drought stress in potato (Yang et al, 2013) and plant developmental regulation in a shrub Amborella trichopoda (Hajieghrari et al, 2015). Sixth miR3979 gene family b stu-miR1030 (a to g): stu-miR1030a, stu-miR1030b, stu-miR1030c, stu-miR1030d, stu-miR1030e, stu-miR1030f and stu-miR1030g; stu-miR1510 (a and b): stu-miR1510a and stu-miR1510b; stu-miR2673 (a and b): stu-miR2673a and stumiR2673b.…”

Section: Target Prediction Of Mirnas and Functional Analysismentioning

confidence: 99%

“…With the discovery of first miRNAs (lin-4 and let-7) in Caenorhabditis elegans (Olsen et al, 1999), in plants first miRNA was identified in Arabidopsis thaliana in 2002 (Park et al, 2002). Since then with the advent of modern computational tools and conserved nature of mature miRNAs, several miRNAs have been identified across the plant species such as potato (Xie et al, 2011;Yang et al, 2013;Zhang et al, 2013), Solanaceae plants (Kim et al, 2011), Arabidopsis (Liang et al, 2015), rice (Campo et al, 2013), wheat (Akdogan et al, 2015), Brassica juncea (Srivastava et al, 2013), chickpea (Kohli et al, 2014), citrus (Lu et al, 2014) and peach (Luo et al, 2013) to name a few. To date, a total of 24,521 distinct mature hairpin precursor miRNAs across 206 species have been annotated in the miRBase Release 20, an open online repository.…”

Section: Introductionmentioning

confidence: 99%

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In silico identification of candidate microRNAs and their targets in potato somatic hybrid Solanum tuberosum (+) S. pinnatisectum for late blight resistance

Singh¹,

Tiwari²,

Rawat³

et al. 2016

Plant Omics

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We employed computational tools to identify candidate miRNAs from 153 putative known genes obtained from a previous microarray experiment by Singh et al. (2016) for late blight resistance. In this study, we identified eight miRNAs families (miR395, 821, 1030, 1510, 2673, 3979, 5021 and 5213) to the nine potato genes. Mature miRNAs located at different positions of pre-miRNAs are varied from 19 to 21 nucleotides in length. Of the total 343 published miRNAs in potato (miRBase), all identified miRNAs family are new to potato crop except miR395. In particular, these novel miRNAs were identified for pathogen-induced late blight resistance mechanism in interspecific potato somatic hybrid. Majority of the predicted target genes (Table 2) of these miRNAs are involved in different biological functions, including disease resistance proteins (NBS-LRR domains) and transcription factors families. This study offers an insight to identify potential candidate miRNAs and their targets to dissect late blight resistance mechanism in potato somatic hybrid Solanum tuberosum (+) S. pinnatisectum.

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“…Eleven known miRNAs targeting mRNAs of enzymes involved in the proline synthesis pathway were predicted by bioinformatic analyses (Yang et al, 2013). Ten out of 11 predicted miRNAs were successfully validated in potato by qRT-PCR assay, and 9 of 10 miRNAs were downregulated and one was upregulated under drought stress conditions.…”

Section: Potato Mirnas In Response To Drought and Heat Stressesmentioning

confidence: 99%

Recent advances in potato genomics, transcriptomics, and transgenicsunder drought and heat stresses: a review

Aksoy

Demirel²,

Öztürk³

et al. 2015

Turk J Bot

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Sustainable potato production practices are crucial for food security and social sustainability in the future since potato is a highly nutritious food and it is considered as one of the most promising crops to reduce human hunger and poverty in the world due to its high yield potential. However, being a temperate crop, potato is exposed to various environmental stresses, including extended periods of drought and heat. The majority of potato genomics, transcriptomics, and transgenics studies concentrate on the characterization of molecular mechanisms governing cold hardiness of tubers and response and tolerance mechanisms against diseases. Likewise, potato breeding studies focus on increasing the yield, extending the postharvest storage, and developing cultivars that withstand biotic stresses. The number of genomics, transcriptomics, and transgenics studies of drought and heat tolerance in potato is limited, although they are necessary state-of-the-art research procedures to characterize and identify the regulatory mechanism underlying any stresses in order to develop new crop varieties that can tolerate harsh environmental conditions. For these reasons, this review focuses on recent advances in genomics, transcriptomics, and transgenics of drought and heat tolerance in potato.

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Prediction and verification of microRNAs related to proline accumulation under drought stress in potato

Cited by 36 publications

References 41 publications

Drought stress‐induced changes in starch yield and physiological traits in potato

Drought stress‐induced changes in starch yield and physiological traits in potato

In silico identification of candidate microRNAs and their targets in potato somatic hybrid Solanum tuberosum (+) S. pinnatisectum for late blight resistance

Recent advances in potato genomics, transcriptomics, and transgenicsunder drought and heat stresses: a review

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