The aim of this study was to determine the effect of a calving season on the Wood's model parameters of the lactation curve and its characteristics in Czech Fleckvieh cows.The observed Czech Fleckvieh cows (with minimum of 75 % of Czech Fleckvieh breed in the genotype) born between 1994-2007 formed three parity groups: parity 1 with 350853 cows, parity 2 with 269276 cows and parity 3 with 175029 cows. All the parity groups were further classified by the calving season: winter (December-February), spring (March-May), summer (June-August) and autumn (September-November). The Wood's function parameters (a, b, c) were calculated and the lactation characteristics estimated were: partial milk yields per 1-100 days, 100-200 days and a total milk yield of 305 days, the peak yield -amount and day, index of persistency 2 : 1 and a coefficient of the declination rate of the lactation curve. The calving season significantly affected the parameters (a, b, c) of the lactation curve, the persistency of lactation and the number of days-in-milk at peak yield; it had no significant effect on the other characteristics. In general, the cows which calved in winter and autumn had more persistent lactations, regardless of the number of lactation. The first-parity cows reached the peak yield sooner when they calved in summer, the older cows in summer and autumn.