Prediction of acute rejection in kidney transplanted patients based on the point-of-care isothermal molecular diagnostics platform

Open Thermal Control System for Stable Polymerase Chain Reaction on a Digital Microfluidic Chip

“…The polymerase chain reaction (PCR) technology, which enables in vitro amplification of target nucleic acid sequences, , is widely recognized as the gold standard in the molecular biology technique due to its sensitivity and accuracy . Compared to bulky and expensive PCR thermal cyclers, , the point-of-care testing (POCT) devices based on the microfluidic chip technology (also known as lab-on-a-chip) have garnered significant attention. − These devices offer advantages such as reduced reagent volume, rapid reaction time, easy-to-use, and portability.…”

“… 3 Compared to bulky and expensive PCR thermal cyclers, 4 , 5 the point-of-care testing (POCT) devices based on the microfluidic chip technology (also known as lab-on-a-chip) have garnered significant attention. 6 − 8 These devices offer advantages such as reduced reagent volume, rapid reaction time, easy-to-use, and portability.…”

Multiple on-line active valves based centrifugal microfluidics for dynamic solid-phase enrichment and purification of viral nucleic acid

“…[5][6][7][8][9][10][11][12] Among these advancements, centrifugal microfluidics stands out as a technique that combines microfluidic principles with centrifugal force. [13][14][15][16] This innovation arranges microfluidic channels and reaction chambers on a rotating disk, harnessing the mechanical effects of centrifugal force, Coriolis force, and Euler force generated during disk rotation. [17][18][19][20][21] This enables precise manipulation and operation of samples and reagents.…”