Prediction of adsorption isotherms from breakthrough curves

Poursaeidesfahani, Ali; Andrés-García, Eduardo; Lange, Martijn de; Torres‐Knoop, Ariana; Rigutto, Marcello; Nair, Nitish; Gascón, Jorge; Dubbeldam, David; Vlugt, Thijs J. H.

doi:10.1016/j.micromeso.2018.10.037

Cited by 46 publications

(19 citation statements)

References 43 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Adsorption isotherms and breakthrough curves can be built to determine the adsorption of target protein to the ligand immobilized on the chromatographic matrix (Chase 1984). Otherwise, adsorption isotherms can be predicted from experimentally measured breakthrough curves (Poursaeid et al 2019).…”

Section: Affinity Chromatography Performancementioning

confidence: 99%

Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification

Barredo-Vacchelli

Giudicessi

Martínez‐Ceron

et al. 2021

Int J Pept Res Ther

View full text Add to dashboard Cite

The interest in therapeutic monoclonal antibodies (mAbs) has significantly grown in the pharmaceutical industry, exceeding 100 FDA mAbs approved. Although the upstream processing of their industrial production has been significantly improved in the last years, the downstream processing still depends on immobilized protein A affinity chromatography. The high cost, low capacity and short half-life of immobilized protein A chromatography matrices, encouraged the design of alternative short-peptide ligands for mAb purification. Most of these peptides have been obtained by screening combinatorial peptide libraries. These low-cost ligands can be easily produced by solid-phase peptide synthesis and can be immobilized on chromatographic supports, thus obtaining matrices with high capacity and selectivity. Furthermore, matrices with immobilized peptide ligands have longer half-life than those with protein A due to the higher stability of the peptides. In this review the design and synthesis of peptide ligands, their immobilization on chromatographic supports and the evaluation of the affinity supports for their application in mAb purification is described.

show abstract

Section: Affinity Chromatography Performancementioning

confidence: 99%

Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification

Barredo-Vacchelli

Giudicessi

Martínez‐Ceron

et al. 2021

Int J Pept Res Ther

View full text Add to dashboard Cite

show abstract

“…Isoterm adsorpsi merupakan hasil percobaan adsorpsi statis dimana adsorben dan adsorbat dikontakkan selama waktu tertentu sehingga mencapai kesetimbangan [16]. Dalam isoterm adsorpsi proses tersebut digambarkan dengan sebuah persamaan atau rumus.…”

Section: Isoterm Adsorpsiunclassified

Ekstraksi Pektin dari Kulit Jeruk dan Kulit Pisang sebagai Biosorben pada Proses Adsorpsi Logam Berat Fe

Rahayu

Harisma

Syamsuddin

et al. 2021

JSE

View full text Add to dashboard Cite

One of the alternatives in the treatment of waste containing heavy metals is the use of natural materials as adsorbents, which is known as the biosorption process Pectin is one of the components found in plant cell wall compounds which contain many active groups that plays a key role in the biosorption process which can be used as biosorbents. In this research, the raw materials for pectin resources were orange peel and banana peel. The effectiveness of pectin for both ingredients will be analyzed on the basis of 2 variables, namely the length of contact time (60, 90, 120, and 150 minutes) and the mass of pectin used (20, 30, 40, and 50 mg). The pectin of orange and banana peel was proven by Fourier Transform Infrared (FTIR) analysis shown by C=O functional group of aldehyde and carboxylic acid compounds. The results of this study indicated that the optimum condition was 150 minutes contact with the addition of 50 mg pectin giving the best adsorption efficiency for orange peel pectin of 88,096% and banana peel pectin of 99,428%. From the isothermal analysis, the Langmuir and Freundlich isotherms can be applied to the adsorption of Fe metals using orange peel and banana peel pectin with a coefficient of determination of R2 ≥ 0.9.

show abstract

“…The LDF mass transfer coefficient can be calculated combining the coefficient of external mass transfer resistance and intraparticle surface resistance as in Eq. (3) (Poursaeidesfahani et al, 2018;Shafeeyan et al, 2014;Siahpoosh et al, 2009).…”

Section: Kinetic Modelmentioning

confidence: 99%

“…The breakthrough time of the system increases from 4065 to 7590 sec when the bed height is increased from 0.64 to 0.96 m. The length where most of the adsorbates are removed are called the adsorption zone and it is arbitrary depending on the amount of solutes in the bulk fluid (Gabelman, 2017). As the fluid traverses in the column, it will be further adsorb and the column is slowly being saturated with the solute (Poursaeidesfahani et al, 2019). Hence, increasing the adsorption zone like a slow wave.…”

Section: Column Heightmentioning

confidence: 99%

Recovery of Minor Palm Oil Compounds Using Packed Bed Adsorption Column

Bahrun

Bono

Dzilrazman

et al. 2020

JBAT

View full text Add to dashboard Cite

Carotene and tocopherol are valuable products that exist as minor compounds in palm oil and mostly extracted out during many stages of palm oil processing. Hence, most of it ended up in wastewater or palm oil mill effluent (POME). Fortunately, adsorption is potentially one of the most efficient method as compared to the others. In fact, it is widely studied in laboratory scale, in order to obtain equilibrium data for the steady state system. However, industrial practices are mostly operated in unsteady state in a continuous manner. Consequently, this study is executed to design a recovery process of one of the minor compounds in palm oil mill effluent (POME), which is carotene, using silica gel. It aims to predict the dynamic adsorption of recovery of minor compounds from palm oil mill effluent based on available equilibrium data, investigate the effects of dynamic and physical properties of the system towards the process by analyzing the breakthrough curve and study the feasibility of the scale up process by performing a sensitivity analysis on the system. Then, a base simulation was prepared by using available equilibrium data. Operating and design parameters such as, bed height, inlet flowrate and concentration were manipulated. Consistent with previous packed column studies, increase flow and concentration will reduce the time required for the column to achieve saturation, while increase bed height effects were vice versa. Finally, the last objective to achieve was to study the practicality of the packed bed column and perform a sensitivity on assumptions and predictions such as predicted mass transfer coefficient and isotherm model. It is proven that the selection of isotherm model and prediction in coefficient did not pose a large impact to the breakthrough curve and the average time required for the column of 1.5 m tall and 0.8 in diameter, to reach breakthrough time is 1.7 days. Hence, it can be concluded that adsorption technology using silica gel as its adsorbent can be applied is recovering minor compounds in palm oil mills.

show abstract

Prediction of adsorption isotherms from breakthrough curves

Cited by 46 publications

References 43 publications

Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification

Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification

Ekstraksi Pektin dari Kulit Jeruk dan Kulit Pisang sebagai Biosorben pada Proses Adsorpsi Logam Berat Fe

Recovery of Minor Palm Oil Compounds Using Packed Bed Adsorption Column

Contact Info

Product

Resources

About