Prediction of anticancer peptides against MCF-7 breast cancer cells from the peptidomes of Achatina fulica mucus fractions

E-kobon, Teerasak; Thongararm, Pennapa; Roytrakul, Sittiruk; Meesuk, Ladda; Chumnanpuen, Pramote

doi:10.1016/j.csbj.2015.11.005

Cited by 75 publications

(58 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The study of Teerasak et al revealed that two HPLC-separated fractions of Achatina fulica mucus decreased viability of mammary gland adenocarcinoma cells (MCF7) and epithelial cells (Vero) [ 6 ]. In fractions, were identified 16 putative cationic and amphipathic anticancer peptides.…”

Section: Discussionmentioning

confidence: 99%

“…Extracts from tissues of H. aspersa Müller were shown to possess anticancer activity against breast cancer cells (Hs578T) [ 5 ]. Two fractions of mucus of another snail, the giant African snail Achatina fulica , decreased viability of breast cancer cells (MCF-7) [ 6 ]. In turn, the mucus of Actinia equina showed cytotoxic effect on human erythromyeloid leukemia-derived cells (K592) [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Influence of Extracts from Snail Helix aspersa Müller on the Colon Cancer Cell Line Caco-2

Matusiewicz

Kosieradzka

Niemiec

et al. 2018

IJMS

View full text Add to dashboard Cite

Colorectal cancer is the third most widely diagnosed cancer. Extracts from snails may modulate growth and development of colorectal cancer cells. The objective of this study was to determine the chemical composition of tissues derived from Helix aspersa Müller and red-ox properties of tissue extracts. Then, the influence of extracts and their fractions of different molecular weights on viability of Caco-2 cells was examined. Tissue lyophilisates contained antioxidants that could be important in the prevention of colorectal cancer. Moreover, we confirmed the presence of a wide array of compounds that might be used in treatment of this disease. The decrease of cell viability after the application of extracts from lyophilized mucus and foot tissues was affirmed. The effect of extract from mucus could be related to the content of some proteins and peptides, proper essential amino acids (EAA)/non-essential amino acids (NEAA) ratio, Met restriction and the presence of Cu, Ca, Zn, Se. The influence of the extract from foot tissues could be assigned additionally to the presence of eicosapentaenoic, α-linolenic, linoleic and γ-linolenic acids. The opposite effect was demonstrated by extract from lyophilized shells which increased cell viability. Further studies are needed to know whether dietary supplying of H. aspersa Müller tissues can be used as an approach in colorectal cancer management.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro Influence of Extracts from Snail Helix aspersa Müller on the Colon Cancer Cell Line Caco-2

Matusiewicz

Kosieradzka

Niemiec

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…We utilized the Balanced 1 (B1) and Balanced 2 (B2) datasets described previously [ 53 ] to generate a new dataset called the Tyagi-B dataset. In total, we obtained 450 ACPs (225 each from B1 and B2) and 450 non-ACPs (225 each from B1 and B2) by combining both the B1 and B2 datasets.…”

Section: Methodsmentioning

confidence: 99%

MLACP: machine-learning-based prediction of anticancer peptides

et al. 2017

View full text Add to dashboard Cite

Cancer is the second leading cause of death globally, and use of therapeutic peptides to target and kill cancer cells has received considerable attention in recent years. Identification of anticancer peptides (ACPs) through wet-lab experimentation is expensive and often time consuming; therefore, development of an efficient computational method is essential to identify potential ACP candidates prior to in vitro experimentation. In this study, we developed support vector machine- and random forest-based machine-learning methods for the prediction of ACPs using the features calculated from the amino acid sequence, including amino acid composition, dipeptide composition, atomic composition, and physicochemical properties. We trained our methods using the Tyagi-B dataset and determined the machine parameters by 10-fold cross-validation. Furthermore, we evaluated the performance of our methods on two benchmarking datasets, with our results showing that the random forest-based method outperformed the existing methods with an average accuracy and Matthews correlation coefficient value of 88.7% and 0.78, respectively. To assist the scientific community, we also developed a publicly accessible web server at www.thegleelab.org/MLACP.html.

show abstract

“…Snail slime has become a remarkable ingredient in cosmetic products because it contains some biochemical substances, which have properties useful to skin care, such as alantoin, elastin, collagen and hyaluronic acid [5] . Furthermore, the slime has also been reported to have pharmacological activities including antibacterial activity [6,7] , antioxidant and regenerative properties [8] and anticancer property [9] . Interestingly, the slime extracted from Achatina fulica has been reported to heal incised wounds faster than self-healing can [10] .…”

mentioning

confidence: 99%

Antiinflammatory Activity of Slime Extract from Giant African Snail (Lissachatina fulica)

Wiya¹,

Nantarat²,

Saenphet³

2020

Indian J Pharm Sci

View full text Add to dashboard Cite

The present study aims to investigate the antiinflammatory activity of the slime extracts from the giant African snail, Lissachatina fulica. In this study, aqueous and ethanol extracts of the snail slime were evaluated for antiinflammatory activities in vitro. The aqueous extract showed greater antiinflammatory activities than the ethanol extract. The aqueous extract was further tested for antiinflammatory activity in vivo. For acute inflammation, the ear oedema assay was used to screen the slime extract. The results revealed that application of 2 mg/ear extract or phenylbutzone could significantly reduce oedema when compared with ethanol-treated ears. For chronic inflammatory test, the cotton pellet granuloma model was employed to determine the activity of the slime extract. It was found that 2.5 and 6.25 % extract could significantly reduce transudative and granuloma weights. Moreover, the extracts also reduced malondialdehyde and increased superoxide dismutase in the granuloma tissue of the induced rats. In conclusion, the slime extract showed antiinflammatory activity both in vitro and in vivo. The antiinflammatory activity was also observed against acute and chronic inflammations, which suggested that the slime extracts has the potential to be developed into antiinflammatory products.

show abstract

Prediction of anticancer peptides against MCF-7 breast cancer cells from the peptidomes of Achatina fulica mucus fractions

Cited by 75 publications

References 28 publications

In Vitro Influence of Extracts from Snail Helix aspersa Müller on the Colon Cancer Cell Line Caco-2

In Vitro Influence of Extracts from Snail Helix aspersa Müller on the Colon Cancer Cell Line Caco-2

MLACP: machine-learning-based prediction of anticancer peptides

Antiinflammatory Activity of Slime Extract from Giant African Snail (Lissachatina fulica)

Contact Info

Product

Resources

About