Prediction of Bioactive Peptides from Chlorella sorokiniana Proteins Using Proteomic Techniques in Combination with Bioinformatics Analyses

Abstract

Chlorella is one of the most nutritionally important microalgae with high protein content and can be a good source of potential bioactive peptides. In the current study, isolated proteins from Chlorella sorokiniana were subjected to in silico analysis to predict potential peptides with biological activities. Molecular characteristics of proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and proteomics techniques. A total of eight proteins were identified by proteomics techniques from 10 protein bands of the SDS-PAGE. The predictive result by BIOPEP’s profile of bioactive peptides tools suggested that proteins of C. sorokiniana have the highest number of dipeptidyl peptidase-IV (DPP IV) inhibitors, with high occurrence of other bioactive peptides such as angiotensin-I converting enzyme (ACE) inhibitor, glucose uptake stimulant, antioxidant, regulating, anti-amnestic and antithrombotic peptides. In silico analysis of enzymatic hydrolysis revealed that pepsin (pH > 2), bromelain and papain were proteases that can release relatively larger quantity of bioactive peptides. In addition, combinations of different enzymes in hydrolysis were observed to dispense higher numbers of bioactive peptides from proteins compared to using individual proteases. Results suggest the potential of protein isolated from C. sorokiniana could be a source of high value products with pharmaceutical and nutraceutical application potential.

Production and processing of antioxidant bioactive peptides: A driving force for the functional food market

“…The amino acid composition and sequence of the protein greatly determine the presence of antioxidant peptides with required bioactivities (Tejano et al, 2019). The amino acid sequence of the protein can be obtained from databases, such as UniProtKB and NCBI, if the protein source is studied and reported that the hydrolysate from this protein exhibited particular bioactivity.…”

“…Conventional and bioinformatic approaches are widely employed to investigate in vitro bioactivity and physicochemical properties of antioxidant peptides possibly obtained from selected protein sources (Tejano et al, 2019;Yang et al, 2017). The peptides are inactive since they are fused in their parent proteins via peptide bonds.…”

Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application

“…For organisms like C. reinhardtii, Chlorella spp., P. tricornutum, and some blue-green algae (cyanobacteria), the genome-scale metabolic models are available. The core metabolic models and genome-scale system-level metabolic networks available for (Li et al, 2013(Li et al, , 2014bGao et al, 2014a;Sibi et al, 2014;Wu et al, 2015;Park and Choi, 2018;Vogler et al, 2018;Xing et al, 2018) Bathycoccus prasinos ( (May et al, 2009;Chen et al, 2010;Baba et al, 2011;Nguyen et al, 2011;Longworth et al, 2012;Mastrobuoni et al, 2012;Choi et al, 2013;Chaiboonchoe et al, 2014;Wase et al, 2014;Sithtisarn et al, 2017;Salguero et al, 2019) Chlorella (Lu et al, 2013;Ma et al, 2013;Rosenberg et al, 2014;Li et al, 2015a;Chen et al, 2017;Kumar et al, 2018;Tejano et al, 2019)…”

Plant Antimicrobial Peptides: State of the Art, In Silico Prediction and Perspectives in the Omics Era

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