Prediction of effective elastic modulus for glass microspheres loaded polymer composites

Jeyaprakash, P.; Devaraju, A.

doi:10.1016/j.matpr.2019.08.064

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…In comparison, the microspheres could be aligned to a straight line at the higher Re of 0.133 (Figure C). This phenomenon might be related to the significant difference in elasticity between cells (64–420 Pa) and microspheres (2.45–10.1 GPa) . Since the cells are softer than the microspheres and may squeeze into the microchannels, when they flowed outside the microchannels, they may spread to a wider region due to the pinch flow fractionation effect .…”

Section: Resultsmentioning

confidence: 99%

“…This phenomenon might be related to the significant difference in elasticity between cells (64−420 Pa) 32 and microspheres (2.45−10.1 GPa). 33 Since the cells are softer than the microspheres and may squeeze into the microchannels, when they flowed outside the microchannels, they may spread to a wider region due to the pinch flow fractionation effect. 34 Therefore, it is reasonable that lower flow rates were demanding for cells to reach the same focusing effect as the microspheres.…”

Section: ■ Introductionmentioning

confidence: 99%

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Passive Focusing of Single Cells Using Microwell Arrays for High-Accuracy Image-Activated Sorting

Xu,

Chen,

Yang

et al. 2023

Anal. Chem.

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Sorting single cells from a population was of critical importance in areas such as cell line development and cell therapy. Image-based sorting is becoming a promising technique for the nonlabeling isolation of cells due to the capability of providing the details of cell morphology. This study reported the focusing of cells using microwell arrays and the following automatic size sorting based on the real-time recognition of cells. The simulation first demonstrated the converged streamlines to the symmetrical plane contributed to the focusing effect. Then, the influence of connecting microchannel, flowing length, particle size, and the sample flow rate on the focusing effect was experimentally analyzed. Both microspheres and cells could be aligned in a straight line at the Reynolds number (Re) of 0.027−0.187 and 0.027−0.08, respectively. The connecting channel was proved to drastically improve the focusing performance. Afterward, a tapered microwell array was utilized to focus sphere/cell spreading in a wide channel to a straight line. Finally, a custom algorithm was employed to identify and sort the size of microspheres/K562 cells with a throughput of 1 event/s and an accuracy of 97.8/97.1%. The proposed technique aligned cells to a straight line at low Reynolds numbers and greatly facilitated the image-activated sorting without the need for a high-speed camera or flow control components with high frequency. Therefore, it is of enormous application potential in the field of nonlabeled separation of single cells.

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Section: Resultsmentioning

confidence: 99%