Prediction of GC retention values under various column temperature conditions from temperature programmed data

Abstract

SummaryA numerical approach has been developed for the correlation of retention times (total retention time) with temperature in gas chromatography, which allows the calculation of retention parameters including retention index from data acquired under lvvo or more different temperature program conditions. By using this procedure the optimization of temperature condition can be further achieved, especiallywhen a temperature-programmed run is the most suitable mode in the preliminary development of an analytical method for the analysis of an unknown sample.

Rapid Temperature-Programmed Separation and Retention Prediction on a Novel Packed-Capillary Column in Gas Chromatography

“…Downsizing of the packed column also permits the use of the resulting miniaturized packed column in a modern capillary GC system without any special modification to the instruments, although the compatibility of the packed column to the temperature-programmed operation should be considered as typically reported in several publications dealing with the theoretical prediction of the retention time for separation with temperature programming. [13][14][15][16][17][18][19][20][21] In contrast to a number of papers for a theoretical prediction of the retention in GC, a comprehensive comparison of the compatibility of packed columns having different internal diameters to a rapid temperature program in a modern GC system had been quite limited. [21][22][23] In this work, novel packed-capillary columns were developed with a thin-wall capillary of stainless-steel, and the compatibility to a fast temperature-programmed separation was studied on the basis of a theoretical prediction of the retention by a numerical-integration method.…”

Antimicrobial activity and chemical composition of the essential oil of Hofmeisteria schaffneri

“…The retention indices ( Ia and Ip ) using two different columns (polar and non‐polar) were calculated by means of the Van Den Dool equation [14,15] . The use of the polar and non‐polar columns makes the identification process more certain [16] . Compound concentrations (as percentage content), however, were established with the non‐polar column (SPB‐1) from the FID response without application of correction factors.…”

“…[14,15] The use of the polar and non-polar columns makes the identification process more certain. [16] Compound concentrations (as percentage content), however, were established with the non-polar column (SPB-1) from the FID response without application of correction factors. The identity of some compounds was corroborated by co-elution with available standards.…”