Prediction of HCV load using genotype, liver biomarkers, and clinical symptoms by a mathematical model in patients with HCV infection

Abstract

Hepatitis C virus (HCV) infection is a major public health problem with about 1.75 million new HCV cases and 71 million chronic HCV infections worldwide. The study aimed to evaluate clinical, serological, molecular, and liver markers to develop a mathematical predictive model for the quantification of the HCV viral load in chronic HCV infected patients. In this cross‐sectional study, blood samples were taken from 249 recently diagnosed HCV‐infected subjects and were tested for liver condition, viral genotype, and HCV RNA load. Receiver operating characteristics (ROC) curves and multiple linear regression analysis were used to predict the HCV‐RNA load. Genotype 3 followed by genotype 1 were the most prevalent genotypes in Mashhad, Northeastern Iran. The maximum levels of viral load were detected in the mixed genotype group, and the lowest levels in the undetectable genotype group. The log of the HCV viral load was significantly associated with thrombocytopenia and higher serum levels of alanine transaminase (ALT). In addition, the log HCV RNA was significantly higher in patients with arthralgia, fatigue, fever, vomiting, or dizziness. Moreover, genotype 3 was significantly associated with icterus. A ROC curve analysis revealed that the best cut‐off points for serum levels of aspartate aminotransferase (AST), ALT, and alkaline phosphatase (ALP) were >31, >34, and ≤246 IU/L, respectively. Sensitivity, specificity, and positive predictive values for AST were 87.7%, 84.36%, and 44.6%, for ALT they were 83.51%, 81.11%, and 36%, and for ALP were 72.06%, 42.81%, and 8.3%, respectively. A mathematical regression model was developed that could estimate the HCV‐RNA load. Regression model: log viral load = 7.69 − 1.01 × G3 − 0.7 × G1 + 0.002 × ALT − 0.86 × fatigue.

Burden of parasitic infection and its impact on growth of children with hepatitis C Virus

“…As regards gastrointestinal manifestations, the statistical comparison revealed a significant difference between both groups regarding associated vomiting that was more among the HCV-infected group. The reported vomiting may be attributed to the HCV infection itself due to the anticipated correlation with increase in HCV viral load as suggested previously 20 , or due to the drugs ingestion 21 . Other gastrointestinal symptoms, such as diarrhea and abdominal pain were similarly observed in both investigated groups.Regarding the growth rate that is evaluated by the weight and height percentiles, both were significantly lower in most of the HCV-infected patients group, compared to their control group.…”

Hepatitis C: sociodemographic and clinical profile of patients treated with direct-acting antivirals in a pharmaceutical office

“…The youngest age group (25 to 39 years) had a significantly lower viral load. Some researchers suggest that viral load is significantly associated with the virus genotype, elevated serum ALT levels, thrombocytopenia, arthralgia, fever, vomiting, and dizziness (Basharkhah et al, 2019). Although viral load is considered an important prognostic factor in determining treatment, the relationship between viral load, genotype, and disease progression remains controversial (Basharkhah et al, 2019).…”

“…Some researchers suggest that viral load is significantly associated with the virus genotype, elevated serum ALT levels, thrombocytopenia, arthralgia, fever, vomiting, and dizziness (Basharkhah et al, 2019). Although viral load is considered an important prognostic factor in determining treatment, the relationship between viral load, genotype, and disease progression remains controversial (Basharkhah et al, 2019). Factors related to treatment failure in CHC are related to older age, high hepatitis C viral load, and impaired glucose tolerance at the start of treatment (Ghenea et al, 2020).…”