Prediction of Key Candidate Genes for Platinum Resistance in Ovarian Cancer

Guo, Kaidi; Li, Li

doi:10.2147/ijgm.s338044

Cited by 4 publications

(5 citation statements)

References 26 publications

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“…For example, cancer-associated fibroblasts-derived IL-8 enhances chemoresistance via NF-κB activation in GC [ 42 ], gastric cancer cell-derived exosomes induce autophagy and pro-tumor activation of neutrophils via NF-κB by HMGB1/TLR4 interaction [ 43 ], and alpha B-crystallin promotes GC cells invasion and metastasis via NF-κB-induced EMT. Similar findings have been reported on the drug resistance and epithelial–mesenchymal transition of IFI27 in ovarian cancer [ 44 , 45 ]. In this study, a series of functional experiments have confirmed the regulation of ADAMTS16/NF-κB/IFI27 signaling axis in the development of gastric cancer, suggesting that ADAMTS16 can be regarded as a potential target for the treatment of gastric cancer.…”

Section: Discussionsupporting

confidence: 90%

“…Whether ADAMTS16/NF-κB/IFI27 regulates EMT, TME, and tumor resistance still needs further study. Furthermore, members of the ADAMTS family are proteolytic enzymes of the extracellular matrix (ECM) and secretory proteins [ 44 , 45 ]. In recent years, the degradation of ECM has also been shown to promote tumor development [ 46 , 47 ], but the role of ADAMTS16 in gastric cancer remains unclear.…”

Section: Discussionmentioning

confidence: 99%

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The Novel Protein ADAMTS16 Promotes Gastric Carcinogenesis by Targeting IFI27 through the NF-κb Signaling Pathway

Zhou

Jiang

et al. 2022

IJMS

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A disintegrin and metalloproteinase with thrombospondin motifs 16 (ADAMTS16) has been reported to be involved in the pathogenesis of solid cancers. However, its role in gastric cancer (GC) is unclear. In this study, the role of ADAMTS16 in gastric cancer was investigated. The effects of ADAMTS16 on cell migration, invasion, and proliferation were investigated by functional experiments in vivo and in vitro. Downstream signal pathways of ADAMTS16 were confirmed by using bioinformatics analysis, co-immunoprecipitation, and immunofluorescence. Meanwhile, bioinformatics analysis, qRT-PCR, western blot, and dual-luciferase reporter gene analysis assays were used to identify ADAMTS16 targets. The expression of ADAMTS16 in GC was analyzed in public datasets. The expression of ADAMTS16 and its correlations with the clinical characteristics of GC were investigated by immunohistochemistry. Ectopic ADAMTS16 expression significantly promoted tumor cell migration, invasion, and growth. Bioinformatics analysis and western blot showed that ADAMTS16 upregulated the IFI27 protein through the NF-κb pathway, which was confirmed by immunofluorescence and western blot. Dual-luciferase reporter gene analysis identified a binding site between P65 and IFI27 that may be directly involved in the transcriptional regulation of IFI27. IFI27 knockdown reversed the promoting effect of ADAMTS16 on cell invasion, migration, and proliferation indicating that ADAMTS16 acts on GC cells by targeting the NF-κb/IFI27 axis. ADAMTS16 was associated with poor prognosis in clinical characteristics. ADAMTS16 promotes cell migration, invasion, and proliferation by targeting IFI27 through the NF-κB pathway and is a potential progressive and survival biomarker of GC.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

The Novel Protein ADAMTS16 Promotes Gastric Carcinogenesis by Targeting IFI27 through the NF-κb Signaling Pathway

Zhou

Jiang

et al. 2022

IJMS

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“…For example, cancer-associated broblasts derived IL-8 enhances chemoresistance via NF-κB activation in GC (41); gastric cancer cell-derived exosomes induce autophagy and pro-tumor activation of neutrophils via NF-κB by HMGB1/TLR4 interaction (42) and Alpha B-crystallin promotes GC cells invasion and metastasis via NF-κB-induced EMT. Similar reports have been reported on the drug resistance and epithelial-mesenchymal transition of IFI27 in ovarian cancer (43,44). In this study, a series of functional experiments have con rmed the regulation of ADAMTS16/NF-κB/IFI27 signaling axis in the development of gastric cancer, suggesting that ADAMTS16 can be regarded as a potential target for the treatment of gastric cancer.…”

Section: High Expression Adamts16 Is Associated With Poorer Clinical ...supporting

confidence: 88%

“…Whether ADAMTS16/NF-κB/IFI27 regulates EMT, TME and tumor resistance still needs further study. In addition, ADAMTS family members belong to proteolytic enzymes of extracellular matrix (ECM) and secretory proteins (43,44). In recent years, the degradation of ECM has also been shown to promote tumor development (45,46).…”

Section: High Expression Adamts16 Is Associated With Poorer Clinical ...mentioning

confidence: 99%

The novel protein ADAMTS16 promotes gastric carcinogenesis by targeting IFI27 through the NF-κb signaling pathway

Zhou

Jiang

et al. 2022

Preprint

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Background A disintegrin and metalloproteinase with thrombospondin motifs 16 (ADAMTS16) has been reported to be involved in the pathogenesis of solid cancers. However, its role in gastric cancer (GC) is unclear. In this study, the role of ADAMTS16 in gastric cancer was investigated.Methods The effects of ADAMTS16 on cell migration, invasion and proliferation were investigated by functional experiments in vivo and vitro. Targets of ADAMTS16 were identified using bioinformatics analysis co-immunoprecipitation, immunofluorescence and dual-luciferase reporter gene analysis assays. The expression of ADAMTS16 in GC was analyzed in public datasets. The expression of ADAMTS16 and its correlations with the clinical characteristics of GC were investigated by immunohistochemistry.Results Ectopic ADAMTS16 expression significantly promoted tumor cell migration, invasion, and growth. Bioinformatics analysis and western blotting showed that ADAMTS16 upregulated the IFI27 protein through the NF-κb pathway, which was confirmed by immunofluorescence and western blot. Dual-luciferase reporter gene analysis identified a binding site between P65 and IFI27 that may be directly involved in the transcriptional regulation of IFI27. IFI27 knockdown reversed the promoting effect of ADAMTS16 on cell invasion,migration and proliferation indicating that ADAMTS16 acts on GC cells by targeting the NF-κb/IFI27 axis. ADAMTS16 was associated with poor prognosis in clinical characteristics.Conclusions ADAMTS16 promotes cell migration, invasion and proliferation by targeting IFI27 through the NF-κB pathway and is a potential progressive and survival biomarker of GC.

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“…The expression levels of ITGA5, CRYAB, IFI27 , and CEACAM6 genes were significantly increased in high-grade OC, which promoted OC metastasis ( 44 – 46 ). IFI27 may be related to platinum resistance of OC ( 47 ). Endonuclease G (ENDOG) and BAG1 promote OC cell proliferation ( 48 ) by regulating EGF signaling pathway and enhancing anoikis resistance ( 49 ).…”

Section: Discussionmentioning

confidence: 99%

A signature based on anoikis-related genes for the evaluation of prognosis, immunoinfiltration, mutation, and therapeutic response in ovarian cancer

Duan

2023

Front. Endocrinol.

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BackgroundOvarian cancer (OC) is a highly lethal and aggressive gynecologic cancer, with an overall survival rate that has shown little improvement over the decades. Robust models are urgently needed to distinguish high-risk cases and predict reliable treatment options for OC. Although anoikis-related genes (ARGs) have been reported to contribute to tumor growth and metastasis, their prognostic value in OC remains unknown. The purpose of this study was to construct an ARG pair (ARGP)-based prognostic signature for patients with OC and elucidate the potential mechanism underlying the involvement of ARGs in OC progression.MethodsThe RNA-sequencing and clinical information data of OC patients were obtained from The Center Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. A novel algorithm based on pairwise comparison was utilized to select ARGPs, followed by the Least Absolute Shrinkage and Selection Operator Cox analysis to construct a prognostic signature. The predictive ability of the model was validated using an external dataset, a receiver operating characteristic curve, and stratification analysis. The immune microenvironment and the proportion of immune cells were analyzed in high- and low-risk OC cases using seven algorithms. Gene set enrichment analysis and weighted gene co-expression network analysis were performed to investigate the potential mechanisms of ARGs in OC occurrence and prognosis.ResultsThe 19-ARGP signature was identified as an important prognostic predictor for 1-, 2-, and 3-year overall survival of patients with OC. Gene function enrichment analysis showed that the high-risk group was characterized by the infiltration of immunosuppressive cells and the enrichment of adherence-related signaling pathway, suggesting that ARGs were involved in OC progression by mediating immune escape and tumor metastasis.ConclusionWe constructed a reliable ARGP prognostic signature of OC, and our findings suggested that ARGs exerted a vital interplay in OC immune microenvironment and therapeutic response. These insights provided valuable information regarding the molecular mechanisms underlying this disease and potential targeted therapies.

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Prediction of Key Candidate Genes for Platinum Resistance in Ovarian Cancer

Cited by 4 publications

References 26 publications

The Novel Protein ADAMTS16 Promotes Gastric Carcinogenesis by Targeting IFI27 through the NF-κb Signaling Pathway

The Novel Protein ADAMTS16 Promotes Gastric Carcinogenesis by Targeting IFI27 through the NF-κb Signaling Pathway

The novel protein ADAMTS16 promotes gastric carcinogenesis by targeting IFI27 through the NF-κb signaling pathway

A signature based on anoikis-related genes for the evaluation of prognosis, immunoinfiltration, mutation, and therapeutic response in ovarian cancer

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