Prediction of radiosensitivity of oral cancers by serial cytological assay of nuclear changes

Harnessing the complexity of DNA-damage response pathways to improve cancer treatment outcomes

“…Mitotic catastrophe (MC) has been reported as a prominent response to different anticancer drugs that affect cytoskeletal components and include microtubule toxins (Tounekti et al, 1993;Lock and Stribinskiene, 1996;Torres and Horwitz, 1998;Taylor et al, 2006), radiotherapy (Streffer et al, 1986;Falkvoll, 1990;Zolzer et al, 1995;Bhattathiri et al, 1998;Widel et al, 1999) and chemotherapy (Yashige et al, 1999). Cell cycle checkpoint inhibitors in combination with DNA-damaging agents enhance MC by inducing 'mitotic slippage'.…”

Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

“…In two studies the linear increase of MN number in cancer cells with the dose of radiation was observed [24,30]. In the exfoliated oral tumor cells of the patients with good outcome of radiotherapy the number of cells with MN was significantly higher than in the resistant ones to therapy [28]. In the cervix tumor cells MN have good predictive value after one week of therapy -high number of MN compared to the background level predict good response to radiotherapy [29].…”

“…The most important data of 4 papers in Table 2 are presented concerning the studied cells with no sign of pathology [23][24][25][26] presented (Table 3) [27][28][29][30]. Both in normal and tumor cells a significant increase of MN was observed due to radiation.…”

“…In 4 papers MN level was studied in cervix and oral mucosa tumor cells (Table 3) [24,[27][28][29][30]. In some cases MN frequencies in the tumor cells were higher than in the normal mucosa cells (e. g., 15 ‰ [27] and 11 ‰ [28] compared with the healthy subjects from India -0.7-4.0 ‰ [32]).…”

“…In some cases MN frequencies in the tumor cells were higher than in the normal mucosa cells (e. g., 15 ‰ [27] and 11 ‰ [28] compared with the healthy subjects from India -0.7-4.0 ‰ [32]). In two studies the linear increase of MN number in cancer cells with the dose of radiation was observed [24,30].…”