Prediction of recurrence using exfoliative cytology and melanoma‐associated antigen‐A mRNA analysis following wide excision of oral squamous cell carcinoma: short report

Mollaoğlu, Nur; Metzler, Philipp; Zenk, Johannes; Nkenke, Emeka; Neukam, Friedrich Wilhelm; Ries, Jutta

doi:10.1111/j.1365-2303.2010.00814.x

Cited by 6 publications

(7 citation statements)

References 9 publications

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“…For that reason, this study combined different variables, such as clinical phenotypes and genotypes as well as habits, in order to create models that enable genotypic and phenotypic patterns to be obtained in patients with OPMD, using non-invasive methods such as exfoliative cytology (16). We included oral lichen planus lesions as OPDM.…”

Section: Discussionmentioning

confidence: 99%

Malignancy risk models for oral lesions

Zarate¹,

Brezzo²,

Secchi³

et al. 2013

Med Oral

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Objectives: The aim of this work was to assess risk habits, clinical and cellular phenotypes and TP53 DNA changes in oral mucosa samples from patients with Oral Potentially Malignant Disorders (OPMD), in order to create models that enable genotypic and phenotypic patterns to be obtained that determine the risk of lesions becoming malignant. Study Design: Clinical phenotypes, family history of cancer and risk habits were collected in clinical histories. TP53 gene mutation and morphometric-morphological features were studied, and multivariate models were applied. Three groups were estabished: a) oral cancer (OC) group (n=10), b) OPMD group (n=10), and c) control group (n=8). Results: An average of 50% of patients with malignancy were found to have smoking and drinking habits. A high percentage of TP53 mutations were observed in OC (30%) and OPMD (average 20%) lesions (p=0.000). The majority of these mutations were GC ? TA transversion mutations (60%). However, patients with OC presented mutations in all the exons and introns studied. Highest diagnostic accuracy (p=0.0001) was observed when incorporating alcohol and tobacco habits variables with TP53 mutations. Conclusions: Our results prove to be statistically reliable, with parameter estimates that are nearly unbiased even for small sample sizes. Models 2 and 3 were the most accurate for assessing the risk of an OPMD becoming cancerous. However, in a public health context, model 3 is the most recommended because the characteristics considered are easier and less costly to evaluate. Key words:TP53, oral potentially malignant disorders, risk factors, genotype, phenotype.

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Section: Discussionmentioning

confidence: 99%

Malignancy risk models for oral lesions

Zarate¹,

Brezzo²,

Secchi³

et al. 2013

Med Oral

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“…Additionally, the problem of the limited reproducibility of incision biopsies within the whole lesion may be solved. Recent studies already point to the possible application of the combination of the cytobrush method and MAGE-A expression analyses in the early diagnoses of OSCC, risk assessment of OLP and the detection of possible early recurrences by close follow-up after surgery (25,42). This tool may help to increase the patient survival rate and quality of life.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of MAGE-A expression and grade of dysplasia for predicting malignant progression of oral leukoplakia

Ries

Agaimy

Vairaktaris

et al. 2012

International Journal of Oncology

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The risk of the malignant transformation of oral leukoplakia (OLP) is difficult to predict by histopathology. Melanoma-associated antigen-A (MAGE-A) expression is restricted to malignant cells and may be useful for the more accurate estimation of the potential malignant transformation of pre-malignant lesions. The aim of the present study was to investigate whether the expression of MAGE-A can be used to predict the malignant transformation of OLP. Paraffin-embedded tissue samples of OLP from 74 patients followed-up for at least 5 years were included. A total of 24 progressing and 50 non-progressing OLP, 18 corresponding tumor and 30 healthy mucosa specimens were analysed for MAGE-A 1, 3, 4, 6 10 and 12 expression by nested real‑time RT-PCR and graded for dysplasia. In total, 46% of the progressing lesions expressed at least 1 out of the examined MAGE-A antigens, whereas no expression was detected in any of the non-progressing OLP and normal specimens. The correlation between malignant transformation and MAGE-A expression was statistically significant (p=0.00001). Furthermore, 42% of the progressing OLPs without dysplasia (D0) expressed at least 1 antigen. The correlation between the grade of dysplasia and MAGE-A staining in the malignant transformation group was not significant (p=0.08). The detection of at least 1 MAGE-A antigen may allow the identification of high-risk lesions that may progress into carcinoma with time. Therefore, the investigation of MAGE-A expression should be assessed in order to obtain a more accurate evaluation of the potential cancer risk of OLP.

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“…However, the explosion of knowledge that is improving our understanding of the complex biology of diseases is providing cytopathologists with the possibility to go further using molecular technology applied to exfoliated or aspirated cells 1,2 . In this issue of Cytopathology , several papers address the use of different types of molecular technique in exfoliative and aspiration cytology for the study of diagnostic, prognostic and predictive markers 3–10 . These papers cover a range of applications of techniques such as flow cytometry, 3,4,10 fluorescence in situ hybridization (FISH), 10 polymerase chain reaction (PCR), 7,9 reverse transcription‐polymerase chain reaction (RT‐PCR) 8 and sequencing 7,9 to study DNA and mRNA alterations in exfoliated cells from serous fluids 3,4,6 or obtained by cytobrush, 8 as well as in cells from fine needle aspiration (FNA) 5,7,9,10 .…”

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confidence: 99%

“…deal with the identification of infectious agents 5 . Kim et al , Schmid et al , Mollaoglu et al and Pang et al deal with the detection of genetic alterations that can contribute to the diagnosis of tumours, 10 detection of recurrence, 8 or that will even guide therapy 9 …”

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confidence: 99%

“…Readers of this issue of Cytopathology will certainly find valuable information on the utility of these new methods and the feasibility of their introduction into cytology laboratories. However, it is remarkable to note how many different types of cytological preparation were used in these different studies: fresh material, 3,4,6,8 liquid‐based cytology preparations, 10 previously stained alcohol‐fixed and air‐dried smears 5,7,9,10 and cell‐blocks 9,10 . A recent multi‐institutional inquiry raised an interesting discussion concerning the application of immunocytochemistry techniques in European laboratories, showing the extreme variability in the use of the techniques as well as the lack of standardization 11 .…”

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confidence: 99%

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