Soil salinization is an ecological challenge across the world. Particularly in arid and semi-arid regions where evaporation is rapid and rainfall is scarce, both primary soil salinization and secondary salinization due to human activity pose serious concerns. Soil is subject to various human disturbances in Xinjiang in this area. Samples with a depth of 0–10 cm from 90 soils were taken from three areas: a slightly disturbed area (Area A), a moderately disturbed area (Area B), and a severely disturbed area (Area C). In this study, we first calculated the hyperspectral reflectance of five spectra (R, R, 1/R, lgR, 1/lgR, or original, root mean square, reciprocal, logarithm, and reciprocal logarithm, respectively) using different fractional-order differential (FOD) models, then extracted the bands that passed the 0.01 significance level between spectra and total salt content, and finally proposed a partial least squares regression (PLSR) model based on the FOD of the significance level band (SLB). This proposed model (FOD-SLB-PLSR) is compared with the other three PLSR models to predict with precision the total salt content. The other three models are All-PLSR, FOD-All-PLSR, and IOD-SLB-PLSR, which respectively represent PLSR models based on all bands, all fractional-order differential bands, and significance level bands of the integral differential. The simulations show that: (1) The optimal model for predicting total salt content in Area A was the FOD-SLB-PLSR based on a 1.6 order 1/lgR, which provided good predictability of total salt content with a RPD (ratio of the performance to deviation) between 1.8 and 2.0. The optimal model for predicting total salt content in Area B was a FOD-SLB-PLSR based on a 1.7 order 1/R, which showed good predictability for total salt content with RPDs between 2.0 and 2.5. The optimal model for predicting total salt content in Area C was a FOD-SLB-PLSR based on a 1.8 order lgR, which also showed good predictability for total salt content with RPDs between 2.0 and 2.5. (2) Soils subject to various disturbance levels had optimal FOD-SLB-PLSR models located in the higher fractional order between 1.6 and 1.8. This indicates that higher-order FODs have a stronger ability to extract feature data from complex information. (3) The optimal FOD-SLB-PLSR model for each area was superior to the corresponding All-PSLR, FOD-All-PLSR, and IOD-SLB-PLSR models in predicting total salt content. The RPD value for the optimal FOD-SLB-PLSR model in each area compared to the best integral differential model showed an improvement of 9%, 45%, and 22% for Areas A, B, and C, respectively. It further showed that the fractional-order differential model provides superior prediction over the integral differential. (4) The RPD values that provided an optimal FOD-SLB-PLSR model for each area were: Area A (1.9061) < Area B (2.0761) < Area C (2.2892). This indicates that the prediction effect of data processed by fractional-order differential increases with human disturbance increases and results in a higher-precision model.