Prediction of Survival in Diffuse Large-B-Cell Lymphoma Based on the Expression of Six Genes

Abstract

Measurement of the expression of six genes is sufficient to predict overall survival in diffuse large-B-cell lymphoma.

Gene expression profiling identifies molecular subgroups among nodal peripheral T-cell lymphomas

“…The PTCL-U1 gene expression signature was characterized by overexpression of genes known to be associated with poor outcome and/or aggressive behavior in various tumor cells, such as overexpression of CCND2 (Lossos et al, 2004). Overexpression of SIX3, coding for a homeotic coactivator of nuclear receptor, is also known to deregulate expression of target genes towards uncontrolled tumor growth (Hisaoka et al, 2004).…”

“…Their expression level was analysed by RQ-PCR in eight relevant T-NHL and in normal PB lymphocyte (PBL) mRNA samples. GAPDH was used as the endogenous RNA and cDNA quantity control (Lossos et al, 2004). Total RNA (2.5 mg) was subjected to reverse transcription using random primers and Superscript II (Invitrogen).…”

Comparative and integrative functional genomics of HCC

“…This approach promises to provide diagnostic and prognostic markers that can be clinically used in the near future (Lossos et al, 2004). The research focus has now shifted toward identifying genetic determinants that are components of the specific regulatory pathways altered in cancers, leading to the discovery of novel therapeutic targets.…”

Prediction of survival of diffuse large B‐cell lymphoma patients via the expression of three inflammatory genes

“…To determine the correlation between inflammatory genes and genes correlated with the survival of DLBCL, 34 survival genes from the previous study which were measured with microarrays or quantitative RT‐PCR either individually or in large data sets to predict survival in DLBCL patients were found 8. 34 inflammatory cytokines from the opened results of research were included in this study, which could mediate direct interactions between cells and regulate processes taking place in the extracellular environment, and play a significant role in the multi‐faceted response to inflammatory disease as well as cancers 7, 22, 23, 26, 27, 28, 29.…”

“…studied 36 genes whose expressions were reported to predict prognosis of DLBCL 8. By using real‐time RT‐PCR to detect the mRNA expression level of each gene from independent samples of 66 patients and applying the univariate analysis, they selected out six genes including LMO2, BCL6, CCND2, FN1, SCYA3 , a nd BCL2 based on the rank of the gene's prediction ability and constituted a prediction model based on their mRNA expression level, which was independent of IPI and sufficient to predict the survival of DLBCL patients 8.…”