Prediction of the glass transition in aqueous solutions of simple amides by molecular dynamics simulations

Kreck, Cara A.; Mandumpal, Jestin Baby; Mancera, Ricardo L.

doi:10.1016/j.cplett.2010.11.009

Cited by 18 publications

(10 citation statements)

References 42 publications

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“…9,13,14 DMSO is one of the most widely used and important CPAs due to its ability to diffuse through cell membranes 15 coupled with its effect on promoting the vitrication of aqueous solutions. 16,17 The formation of a glassy state of water upon rapid cooling of the sample reduces the likelihood of the formation of intracellular ice crystals, which can cause signicant damage to cells. DMSO has been shown to have other interesting properties, such as the ability to induce cell fusion 18 and act as an anti-inammatory 19 and anesthetic.…”

Section: Introductionmentioning

confidence: 99%

Molecular dynamics simulations of mixed DOPC–β-sitosterol bilayers and their interactions with DMSO

Hughes

Mancera

2013

Soft Matter

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Section: Introductionmentioning

confidence: 99%

Molecular dynamics simulations of mixed DOPC–β-sitosterol bilayers and their interactions with DMSO

Hughes

Mancera

2013

Soft Matter

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“…The success of the cryopreservation of plant materials will depend on the combination of several compounds that can prevent further explant dehydration and facilitate vitrification during direct immersion in liquid nitrogen (Brison et al 1995, Bekheet et al 2020. It has been shown that the addition of DMSO and ethylene glycol to cryoprotectant solutions can increase the stability of the vitreous state of the water in the cell membrane, thus reducing intracellular ice formation (Turner et al 2001, Kreck et al 2011, Malajczuk et al 2013. Both DMSO and ethylene glycol are penetrating cryoprotectants that show more rapid permeability through cell walls and the protoplast than compounds such as sucrose and glycerol (Tao andLi 1986, Volk et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Influence of ethylene glycol on Eucalyptus grandis cryopreservation using the V cryo-plate technique

Oliveira

Souza

Carvalho

et al. 2022

Crop Breed. Appl. Biotechnol.

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The conservation of plant genetic resources is fundamental for the development of agriculture. The development of efficient cryopreservation protocols has become an effective tool to preserve cells, tissues and organs of different plant species. We sought here to develop an efficient method of cryopreservation of Eucalyptus grandis employing the V cryo-plate technique. This experiment examined the exposure of shoot tips to three different cryoprotectants (PVS2, PVS3, and PVS4). The cryoprotectants PVS2 and PVS4 proved to be more efficient among the cryoprotectants tested, as 44.4% of the shoot tips immersed in those solutions survived, and 31.1% generated new shoots. Ethylene glycol was found to be a key compound for the successful cryopreservation of shoot tips, in light of its low toxicity as compared to other cryoprotective compounds. Thus, the methodology developed here represents an effective method for the conservation of E. grandis germplasm through cryopreservation and the use of the V cryo-plate technique.

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“…sucrose) are providing insight at the molecular level of the interactions of these agents with phospholipid chains and headgroups and their effect on the liquid crystallinity and stability of biological membranes (Pereira and Hünenberger 2008). In addition, investigation of the vitrification properties of these cryoprotectants on liquid water is shedding light on the molecular mechanism of solvent cryoprotection by explaining changes to the glass transition temperature of water following the addition of cryoprotective agents (Kreck et al 2010). Crossdisciplinary research between plant biology and biophysics is thus likely to provide new fundamental results to help rationalise and optimise cryopreservation protocols in the future.…”

Section: Molecular-physiological Studies Linked To Cryopreservationmentioning

confidence: 98%

Cryopreservation of threatened native Australian species—what have we learned and where to from here?

Kaczmarczyk

Turner

Bunn

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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Cryogenic storage techniques have been developed and adopted for more than 100 (mainly agricultural) plant species worldwide, and within Australia, at least 30 critically endangered plants have been stored long term using cryogenic approaches. Nevertheless, there are many species that are very difficult to store using current procedures, and organizations involved in plant germplasm conservation (such as botanic gardens, agricultural institutions, etc.) that utilise cryogenic storage techniques are in some respects at a crossroads in their endeavours to cheaply and effectively store a wide selection of species and genotypes for conservation and agricultural/horticultural purposes. For taxa that are not amenable to current cryogenic approaches, new ways of developing cryogenic storage techniques need to be investigated, including research into the ways in which cell membranes interact and change when cooled to cryogenic temperatures (−196°C in liquid nitrogen) in the presence of various cryoprotective agents. This review highlights the current state of cryogenic research both within Australia and internationally, provides a case study on threatened plant species and also describes several new research initiatives that aim to provide answers to why some native species are quite amenable to widely utilised cryogenic approaches whilst others are currently non-responsive. New approaches aim to integrate laboratory and membrane modelling paradigms to provide guidelines for the development of new cryopreservation protocols and to assess the robustness of theoretical models in predicting optimum cryogenic conditions.

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Prediction of the glass transition in aqueous solutions of simple amides by molecular dynamics simulations

Cited by 18 publications

References 42 publications

Molecular dynamics simulations of mixed DOPC–β-sitosterol bilayers and their interactions with DMSO

Molecular dynamics simulations of mixed DOPC–β-sitosterol bilayers and their interactions with DMSO

Influence of ethylene glycol on Eucalyptus grandis cryopreservation using the V cryo-plate technique

Cryopreservation of threatened native Australian species—what have we learned and where to from here?

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