Predictions and measurements of scattering and absorption over broad wavelength ranges in tissue phantoms

Mourant, Judith R.; Fuselier, Tamika; Boyer, James D.; Johnson, Tamara M.; Bigio, Irving J.

doi:10.1364/ao.36.000949

Cited by 485 publications

(331 citation statements)

References 28 publications

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“…From an approximation to Mie scattering theory, it is possible to derive a relation between reduced scattering coefficient and wavelength (22,23), given by Ј s ( ) ϭ A ϪSP , where SP is the scattering power (related to the scattering center size and number density) and A is an amplitude factor (which also depends on scatterer size and number density). The scattering power may reflect variations in breast physiologic composition associated with age and radiographic density due to variations in scattering center sizes.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Interpreting hemoglobin and water concentration, oxygen saturation, and scattering measured in vivo by near-infrared breast tomography

Srinivasan

Pogue

Jiang

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

261

225

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Near-infrared spectroscopic tomography was used to measure the properties of 24 mammographically normal breasts to quantify whole-breast absorption and scattering spectra and to evaluate which tissue composition characteristics can be determined from these spectra. The absorption spectrum of breast tissue allows quantification of (i) total hemoglobin concentration, (ii) hemoglobin oxygen saturation, and (iii) water concentration, whereas the scattering spectrum provides information about the size and number density of cellular components and structural matrix elements. These property data were tested for correlation to demographic information, including subject age, body mass index, breast size, and radiographic density. Total hemoglobin concentration correlated inversely to body mass index, likely because lower body mass indicates proportionately less fat and more glandular tissue, and glandular tissue contains greater vascularity, hence, more total hemoglobin. Optical scattering was correlated to breast diameter, subject age, and radiographic density. In the radiographic density, fatty breasts had low scattering power and extremely dense breasts had higher values. This observation is consistent with low attenuation of conventional x-rays with fat and higher attenuation in glandular tissues. Optically, fatty tissues have large scatterers leading to a low scattering power, whereas glandular or fibrous tissues have more cellular and collagen-based structures that lead to high scattering power. The study presents correlative data supporting the hypothesis that optical measurements of absorption and scattering can provide physiologically relevant information about breast tissue composition. These breast constituents vary significantly between individuals and can be altered because of changes in breast physiology or pathological state.T he breast is a highly heterogeneous and dynamically complex organ whose characteristics depend on factors such as age, hormonal status, habitus, family and medical history, and genetics (1-3). Normal breast tissue changes considerably during development, pregnancy, and menopause, and throughout the menstrual cycle. It has been documented that blood flow can increase up to 50% at the time of ovulation; and by the end of the monthly cycle, some women may undergo breast enlargement of up to 20% because of increased vascularity and water content (1). Total blood content can vary up to a factor of 5 or more between women based on their body-fat content, making the breast one of the most physiologically variable tissues in the human body. With nearinfrared (NIR) spectral imaging, hemoglobin concentration, oxygen saturation, and water content can yield information about the current physiological state of the normal breast.The relatively good transparency of tissue in the red and NIR spectrum (i.e., 600-1,000 nm) permits sufficient light penetration to detect signals through as much as a dozen centimeters of the breast. In the NIR spectrum, the primary absorbers of light are hemoglobin, oxyh...

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Section: Methodsmentioning

confidence: 99%

“…The scattering power may reflect variations in breast physiologic composition associated with age and radiographic density due to variations in scattering center sizes. Typically, large scatterers have lower SP and A values, whereas small scatterers have higher SP and A coefficients (23,24).…”

Section: Methodsmentioning

confidence: 99%

Interpreting hemoglobin and water concentration, oxygen saturation, and scattering measured in vivo by near-infrared breast tomography

Srinivasan

Pogue

Jiang

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

261

225

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“…For a homogeneous sphere of radius r, Mie theory predicts the wavelength dependence of the scattering and the relation between scattering and sphere size. Under the hypothesis that the scattering centers are homogeneous spheres behaving individually, the relationship between |x p ' and wavelength X can be empirically described 19 as…”

mentioning

confidence: 99%

“…The number of photons surviving propagation into a turbid medium as well as their trajectories depend on the optical properties (i.e., absorption and transport scattering coefficients) of the traversed medium. Consequently, the estimates of the optical coefficients obtained by TRS are average values over the volume crossed by photon 19 trajectories and refer to the bulk of the medium and not to the surface layers. In the fruit species and wavelength range considered, in this study, the absorption coefficient did not usually exceed 1 cm -1 and was often much lower (see below), and the transport scattering coefficient was typically lower than 25 cm"" 1 .…”

mentioning

confidence: 99%

Nondestructive quantification of chemical and physical properties of fruits by time-resolved reflectance spectroscopy in the wavelength range 650–1000 nm

Cubeddu¹,

D’Andrea²,

Pifferi³

et al. 2001

Appl. Opt.

152

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Time-resolved reflectance spectroscopy can be used to assess nondestructively the bulk (rather than the superficial) optical properties of highly diffusive media. A fully automated system for time-resolved reflectance spectroscopy was used to evaluate the absorption and the transport scattering spectra of fruits in the red and the near-infrared regions. In particular, data were collected in the range 650-1000 nm from three varieties of apples and from peaches, kiwifruits, and tomatoes. The absorption spectra were usually dominated by the water peak near 970 nm, whereas chlorophyll was detected at 675 nm. For ail species the scattering decreased progressively with increasing wavelength. A best fit to water and chlorophyll absorption line shapes and to Mie theory permitted the estimation of water and chlorophyll content and the average size of scattering centers in the bulls; of intact fruits.

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“…A light-scattering component, s xy ( ), at each pixel (x,y) may be described by a power function [32], [33] and [34], where n 1 according to Refs. [34], [35], [36] and [37].…”

Section: Theoreticalmentioning

confidence: 99%

Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging

Gussakovsky

Yang

Rendell

et al. 2010

Analytical Biochemistry

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Predictions and measurements of scattering and absorption over broad wavelength ranges in tissue phantoms

Cited by 485 publications

References 28 publications

Interpreting hemoglobin and water concentration, oxygen saturation, and scattering measured in vivo by near-infrared breast tomography

Interpreting hemoglobin and water concentration, oxygen saturation, and scattering measured in vivo by near-infrared breast tomography

Nondestructive quantification of chemical and physical properties of fruits by time-resolved reflectance spectroscopy in the wavelength range 650–1000 nm

Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging

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