Predictive capacity of sperm quality parameters and sperm subpopulations on field fertility after artificial insemination in sheep

Santolaria, P.; Vicente-Fiel, S.; Palacín, I.; Fantova, E.; Blasco, M.E.; Silvestre, Mattia; Yániz, J.L.

doi:10.1016/j.anireprosci.2015.10.001

Cited by 70 publications

(52 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sin embargo, la integración de un sistema objetivo de evaluación, junto con la implementación de tinciones fluorescentes que determinan la integridad de la membrana, puede ser un criterio más relevante para determinar el potencial fértil de un eyaculado (8,17). La valoración de los parámetros de cinemática es el reflejo de la capacidad del espermatozoide para migrar a través del tracto genital femenino e interactuar con el oocito en la fertilización (20); una característica que a futuro puede representar una herramienta sistemática útil para la predicción de la calidad seminal de un eyaculado, ya sea para su implementación en inseminación artificial en fresco, o procesos de criopreservación, y que en la actualidad sirve para clasificar los eyaculados en porcentajes de subpoblaciones que tienen alta relación con la fertilidad (4,21).…”

Section: Discussionunclassified

Evaluación de los parámetros de calidad seminal y cinemática espermática en tres razas ovinas de lana en condiciones de trópico alto colombiano

et al. 2017

View full text Add to dashboard Cite

Cómo citar este artículo: CarvajalSerna M, Cortés-López HA, ManriquePerdomo C, Grajales-Lombana HA. Evaluación de los parámetros de calidad seminal y cinemática espermática en tres razas ovinas de lana en condiciones de trópico alto colombiano. Rev Med Vet. 2018;(18) ResumenEl objetivo del presente estudio fue evaluar el efecto del eyaculado, la raza y las colectas sobre los parámetros de calidad seminal y cinemática en 3 tipos raciales ovinos de lana bajo condiciones de trópico alto colombiano. Se colectó semen de 12 machos de la raza criollo, romney marsh y hampshire de 2 a 5 años de edad, con vagina artificial, durante 4 colectas con un tiempo de abstinencia de 3 d. Se realizó el análisis de la motilidad con ayuda de un sistema computarizado de análisis seminal (CASA) en combinación con una tinción de fluorescencia Hoeschst 33259 para el estudio de la viabilidad y la morfología. Se realizó un análisis de medidas repetidas en el tiempo con el paquete estadístico SAS, empleando el método Proc Mixed. No hubo diferencias significativas (p > 0,05) en el porcentaje de motilidad total y motilidad progresiva para el primer y segundo eyaculado, pero sí hubo diferencias (p < 0,05) para la concentración, volumen y viabilidad; el primer eyaculado fue significativamente superior al segundo, independiente de la raza y la colecta. Las variables de cinemática espermática relacionadas con el movimiento progresivo y la velocidad (ALH, VAP, LIN) no difieren entre eyaculados, pero sí hay un efecto racial (p > 0,05) en el que la raza criolla es superior en estas variables, lo que podría sugerir que las células espermáticas de esta raza tienen la capacidad de recorrer mayor distancia en menor tiempo, comparado con los espermatozoides de las otras dos razas, en las condiciones del presente experimento. of total motility and progressive motility for the first and second ejaculates, but there were differences (p < 0.05) for concentration, volume, and viability; the first ejaculate was significantly superior to the second, independent of breed and collection. Sperm kinematic variables related to progressive movement and speed (ALH, VAP, LIN) do not differ between ejaculates, but there is a racial effect (p > 0.05), since the Creole breed is superior in all these variables, which could suggest that the sperm cells of this breed have the ability to travel greater distance in less time, compared with sperm from the other two breeds, under the conditions of the present experiment.Keywords: seminal quality, kinematics, sperm, sheep.Avaliação dos parâmetros de qualidade seminal e cinemática espermática em três raças ovinas de lã em condições de trópico alto colombianoResumo O objetivo deste estudo foi avaliar o efeito da ejaculação, a raça e as coletas sobre os parâ-metros de qualidade seminal e cinemática em 3 tipos raciais ovinos de lã sob condições de trópico alto colombiano. Foram colhidas amostras de sêmen de 12 machos da raça crioulo, romney marsh e hampshire de 2 a 5 anos de idade, com vagina artificial, durante 4 coletas com um tem...

show abstract

Section: Discussionunclassified

Evaluación de los parámetros de calidad seminal y cinemática espermática en tres razas ovinas de lana en condiciones de trópico alto colombiano

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Sperm quality studies conducted to date in order to estimate the potential fertility of semen have been based on the evaluation of different sperm quality tests assessed independently and combined only by using statistical approaches (Santolaria et al, 2015;Sellem et al, 2015). In animal breeding, the final goal is the setting of thresholds to determine which ejaculates and at which concentration are suitable for AI.…”

Section: Discussionmentioning

confidence: 99%

“…In spite of these advances, the predictive capacity of the in vitro analysis on potential fertility of semen is still limited (Rodriguez-Martinez, 2003;Santolaria et al, 2015), although it may be improved using combined statistical analyses of various sperm quality parameters (Sellem et al, 2015;Utt, 2016). In consequence, the complexity of the sperm analysis has progressively been increased with the hope of improving fertility predictions (Fraser et al, 2001;Yániz et al, 2008a).…”

Section: Introductionmentioning

confidence: 99%

Toward an integrative and predictive sperm quality analysis in Bos taurus

Yániz

Soler

Alquézar-Baeta

et al. 2017

Animal Reproduction Science

Self Cite

View full text Add to dashboard Cite

There is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS3Fun kit, were performed. The new method allows a clear discrimination of sperm subpopulations based on membrane and acrosomal integrity, motility and morphology. It was also possible to observe live spermatozoa showing signs of capacitation such as hyperactivated motility and changes in acrosomal structure. Sperm subpopulation with intact plasma membrane and acrosome showed a higher proportion of motile sperm than those with damaged acrosome or increased fluorescence intensity. Spermatozoa with intact plasmalemma and damaged acrosome were static or exhibit weak movement. Significant correlations among the different sperm quality parameters evaluated were also described. We concluded that the ISAS3Fun is an integrated method that represents an advance in sperm quality analysis with the potential to improve fertility predictions.

show abstract

“…Sperm subpopulations in semen have been identified in different species on the basis of biochemistry (humans23), function (boars45), motility (stallions;6 red deer;78 dogs;9 bulls;10 rams;111213 and blue foxes14), and morphometry (stallions;15 boars;1617 red deer;718 bulls;1719 brown bears;20 rams;1213172122 Goeldi's monkey;23 and marmosets24). There is increasing evidence that the heterogeneity of these subpopulations has functional relevance.…”

Section: Introductionmentioning

confidence: 99%

Comparison of different statistical approaches to evaluate morphometric sperm subpopulations in men

et al. 2016

View full text Add to dashboard Cite

This study was designed to characterize morphometric sperm subpopulations in normozoospermic men by using different statistical methods and examining their suitability to classify correctly different sperm nuclear morphologies present in human ejaculates. Ejaculates from 21 normozoospermic men were collected for the study. After semen collection and analysis, samples were prepared for morphometric determination. At least 200 spermatozoa per sample were assessed for sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence. Clustering and discriminant procedures were performed to identify sperm subpopulations from the morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three morphometric subpopulations (large-round 30.4%, small-round 46.6%, and large-elongated 22.9%). In the second analysis, using discriminant methods, the classification was made independently of size and shape. Three morphological categories according to nuclear size (small <10.90 μm2, intermediate 10.91–13.07 μm2, and large >13.07 μm2) and four categories were defined on 400 canonical cells (100 × 4) from 10 men according to sperm nuclear shape (oval, pyriform, round, and elongated). Thereafter, the resulting classification functions were used to categorize 4200 spermatozoa from 21 men. Differences in the class distribution were observed among men from both clustering and discriminant procedures. It was concluded that the combination of CASA-Morph fluorescence-based technology with multivariate cluster or discriminant analyses provides new information on the description of different morphometric sperm subpopulations in normal individuals, and that important variations in the distribution of morphometric sperm subpopulations may exist between men, with possible functional implications.

show abstract

Predictive capacity of sperm quality parameters and sperm subpopulations on field fertility after artificial insemination in sheep

Cited by 70 publications

References 39 publications

Evaluación de los parámetros de calidad seminal y cinemática espermática en tres razas ovinas de lana en condiciones de trópico alto colombiano

Evaluación de los parámetros de calidad seminal y cinemática espermática en tres razas ovinas de lana en condiciones de trópico alto colombiano

Toward an integrative and predictive sperm quality analysis in Bos taurus

Comparison of different statistical approaches to evaluate morphometric sperm subpopulations in men

Contact Info

Product

Resources

About