Nowadays, in the domain of production logistics, one of the most complex planning processes is the accurate forecasting of production and assembly efficiency. In industrial companies, Overall Equipment Effectiveness (OEE) is one of the most common used efficiency measures at semi-automatic assembly lines. Proper estimation supports the right use of resources and more accurate and cost-effective delivery to the customers. This paper presents the prediction of OEE by comparing human prediction with one of the techniques of supervised machine learning through a real-life example. In addition to descriptive statistics, takt time-based decision trees are applied and the target-oriented OEE prediction model is presented. This concept takes into account recent data and assembly line targets with different weights. Using the model, the value of OEE can be predicted with an accuracy of within 1% on a weekly basis, four weeks in advance.