Predictive role of serum and urinary cytokines in invasion and recurrence of bladder cancer

Kumari, Nitu; Agrawal, Usha; Mishra, Ashwani Kumar; Kumar, Anup; Vasudeva, Pawan; Mohanty, N.; Saxena, Sunita

doi:10.1177/1010428317697552

Cited by 44 publications

(48 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, in accordance with other studies, we found that using urine COL4A1 and COL13A1 concentration normalized to urine creatinine (ng/mg Cre) instead of urinary concentration alone (ng/mL) did not improve the diagnostic accuracy. Our results are similar to those of other urinary protein‐based tests, aiming for measurement of soluble proteins related to BCa; for instance, analysis of growth factors, matrix metalloproteases, or other cytokines in urine . The greater the progression of the BCa, the higher the urine collagen concentration and detection sensitivity (Tables ).…”

Section: Discussionsupporting

confidence: 84%

“…As Kumari et al . reported, a panel of serum cytokines had a predictive role in the invasion and recurrence of BCa; serum can be a useful source of non‐invasive diagnostic and prognostic biomarkers for BCa. Kang et al .…”

Section: Discussionmentioning

confidence: 99%

“…Our results are similar to those of other urinary protein-based tests, aiming for measurement of soluble proteins related to BCa; for instance, analysis of growth factors, matrix metalloproteases, or other cytokines in urine. (3,26) The greater the progression of the BCa, the higher the urine collagen concentration and detection sensitivity (Tables 1,2). As Kumari et al reported, a panel of serum cytokines had a predictive role in the invasion and recurrence of BCa; (26) serum can be a useful source of non-invasive diagnostic and prognostic biomarkers for BCa.…”

Section: Discussionmentioning

confidence: 99%

“…(3,26) The greater the progression of the BCa, the higher the urine collagen concentration and detection sensitivity (Tables 1,2). As Kumari et al reported, a panel of serum cytokines had a predictive role in the invasion and recurrence of BCa; (26) serum can be a useful source of non-invasive diagnostic and prognostic biomarkers for BCa. Kang et al demonstrated the potential clinical utility of serum COL6A3 in pancreatic ductal adenocarcinoma.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Diagnostic and prognostic role of urinary collagens in primary human bladder cancer

et al. 2017

View full text Add to dashboard Cite

Collagen type 4 alpha 1 (COL4A1) and collagen type 13 alpha 1 (COL13A1) produced by urothelial cancer cells support the vital oncogenic property of tumor invasion. We investigated the diagnostic and prognostic capability of COL4A1 and COL13A1 in voided urine and compared the observed values with those of fragments of cytokeratin‐19 (CYFRA21‐1), nuclear matrix protein 22 (NMP‐22), and voided urine cytology in bladder cancer (BCa). We collected voided urine samples from 154 patients newly diagnosed with BCa, before surgery and from 61 control subjects. Protein levels of COL4A1, COL13A1, CYFRA21‐1, and NMP‐22 in urine supernatants were measured using enzyme‐linked immunosorbent assays. Diagnostic performance and optimal cut‐off values were determined by receiver operating characteristic analysis. Urine levels of COL4A1, COL13A1, the combined values of COL4A1 and COL13A1 (COL4A1 + COL13A1), and CYFRA21‐1 were significantly elevated in urine from patients with BCa compared to the controls. Among these biomarkers, the optimal cut‐off value of COL4A1 + COL13A1 at 1.33 ng/mL resulted in 57.4%, 83.7%, 56.1%, 80.7%, and 91.7% sensitivity for low‐grade tumors, high‐grade tumors, Ta, T1, and muscle invasive disease, respectively. We evaluated the prognostic value of preoperative urine levels in 130 non‐muscle invasive BCa samples after the initial transurethral surgery. A high urinary COL4A1 + COL13A1 was found to be an independent risk factor for intravesical recurrence. Although these data need to be externally validated, urinary COL4A1 and COL13A1 could be a potential diagnostic and prognostic biomarker for BCa. This easy‐to‐use urinary signature identifies a subgroup of patients with a high probability of recurrence and progression in non‐muscle invasive and muscle invasive BCa.

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Diagnostic and prognostic role of urinary collagens in primary human bladder cancer

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Similar to our results, Kumari et al reported that urine level of GM-CSF was significantly higher in high-grade bladder cancer patients compared to low-grade bladder cancer patients and controls. 26 In the present study, all the patients were diagnosed with high-grade bladder cancer. GM-CSF in the urine of patients with bladder cancer may indicate poor prognosis.…”

Section: Discussionmentioning

confidence: 86%

Colony-stimulating factors detected in tumor cells and voided urine are potential prognostic markers for patients with muscle-invasive bladder cancer undergoing radical cystectomy

Morizawa¹,

Miyake²,

Shimada³

et al. 2018

RRU

View full text Add to dashboard Cite

BackgroundThe clinical use of macrophage colony-stimulating factor, granulocyte colony-stimulating factor (G-CSF), and granulocyte macrophage colony-stimulating factor (GM-CSF) has improved the safety of cytotoxic chemotherapy. However, the overexpression of these CSFs in cancers has been reported to be associated with a poor prognosis in various malignancies. We evaluated the potential of CSF expression as a predictor of clinical outcome in patients with muscle-invasive bladder cancer (MIBC).MethodsConsecutive patients (n=58) with MIBC who underwent radical cystectomy (RC) were included in this retrospective study. Treatment-naïve tumor specimens obtained by initial transurethral resection of bladder tumors prior to RC were immunostained with antibodies against macrophage colony-stimulating factor, G-CSF, and GM-CSF. We compared the clinicopathological variables and survival between these groups. Baseline levels of CSFs in the serum and voided urine were quantified using an enzyme-linked immunosorbent assay and compared with the expression of CSFs in the tumor lesions.ResultsLow expression of GM-CSF in the tumor cells was significantly correlated with a pathological T4 category (vs T2–3; P=0.02). In univariate survival analysis, high G-CSF and low GM-CSF expression in the tumor lesion were associated with poor outcomes. Furthermore, Cox proportional regression analysis revealed that high G-CSF and low GM-CSF expression in the tumor were independent predictors of shorter recurrence-free survival, cancer-specific survival, and overall survival. The levels of CSFs in voided urine were associated with the expression of CSFs in the tumor lesions.ConclusionGM-CSF and G-CSF expression in the tumor lesions obtained by initial transurethral resection are independent predictors of poor outcome in MIBC after RC. Levels of G-CSF and GM-CSF in urine before treatment could be useful in prognostication.

show abstract

Urinary IL‐6 and IL‐8 as predictive markers in bladder urothelial carcinoma: A pilot study

VandenBussche,

Heaney,

Kates

et al. 2023

Cancer Cytopathology

View full text Add to dashboard Cite

BackgroundCytokines are known to be a key a factor in numerous malignancies and to exert an important regulatory role in the tumor microenvironment. Interest has grown in understanding how cytokines modulate the tumor microenvironment and which cytokines may serve as markers of the tumor process; however, a complete picture of the cytokine landscape in bladder cancer remains unclear.MethodsFresh urine specimens with sufficient volume were collected at random intervals. The urine concentrations of IL‐8 (CXCL8), CCL18, and CXCL9 were determined using the standard commercially available enzyme immunoassay. The urine concentrations of IL‐6 were determined using the high sensitivity enzyme immunoassay kit. Urinary cytokine concentrations were normalized with urinary creatinine concentrations.ResultsSignificantly elevated concentrations of IL‐6 and IL‐8 were detected in the urine from patients with urothelial carcinoma on follow‐up compared to patients with benign follow‐up. The presence of both IL‐6 and IL‐8 in the urine samples from the high grade urothelial carcinoma (HGUC) cohort revealed a clear discrimination when compared to samples from patients with benign follow‐up. The presence of the combination of both IL‐6 and IL‐8 had a sensitivity of 90.0% and a specificity of 81.25%. Similar data were obtained when receiver operating characteristic analysis was performed on both IL‐6 and IL‐8 concentrations in the urine from patients with HGUC vs. the hematuria cohort.ConclusionsThe presence of IL‐6 and IL‐8 in urine specimens may have predictive value for urothelial carcinoma. However, a large longitudinal study is required to statistically eliminate confounding factors and support this theory.

show abstract

Predictive role of serum and urinary cytokines in invasion and recurrence of bladder cancer

Cited by 44 publications

References 28 publications

Diagnostic and prognostic role of urinary collagens in primary human bladder cancer

Diagnostic and prognostic role of urinary collagens in primary human bladder cancer

Colony-stimulating factors detected in tumor cells and voided urine are potential prognostic markers for patients with muscle-invasive bladder cancer undergoing radical cystectomy

Urinary IL‐6 and IL‐8 as predictive markers in bladder urothelial carcinoma: A pilot study

Contact Info

Product

Resources

About