Predictive significance of HIF-1α, Snail, and PD-L1 expression in breast cancer

Zubareva, E. Yu.; Сеньчукова, М. А.; Karmakova, Tatyana

doi:10.1007/s10238-023-01026-z

Cited by 2 publications

(2 citation statements)

References 45 publications

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“…The material for the study was tumor samples obtained by trephine biopsy. The expression of PD-L1 in the tumor was studied on the invasive component of puncture biopsy specimens by immunohistochemistry using PD-L1 polyclonal antibodies (dilution 30 μg/mL, Cloud-Clone Corp., China), as described previously [11] . Histological specimens were examined by light microscopy (a Levenhuk D740T digital microscope connected to a 5.1 MP camera, Russia).…”

Section: Immunohistochemical Studymentioning

confidence: 99%

Assessment of PD-L 1 expression in a tumor improves the accuracy of predicting the risk of regional breast cancer metastases

Zubareva,

Senchukova,

Shubin

et al. 2023

Trends Immunother.

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Background: Improving the accuracy of axillary lymph node (ALN) status assessment and the search for new markers associated with the risk of breast cancer (BC) metastasis continues to be an urgent problem. Aim: To establish the prognostic significance of the expression of PD-L1 in a tumor for assessing the risk of BC metastasis in ALNs. Materials and methods: A retrospective, case‒control cohort study included 158 patients aged 30 to 85 years with newly diagnosed BC. The material for the study was tumor samples obtained by trephine biopsy. The expression of PD-L1 in the tumor was studied on the invasive component of puncture biopsy specimens by immunohistochemistry using PD-L1 polyclonal antibodies. Statistical analysis was performed using Statistica 12.0 software. Receiver operating characteristic (ROC) curves for PD-L1 and Ki67 were constructed to discriminate cases with and without metastases in the ALNs. Univariate and multivariate analyses were performed to establish independent predictors associated with the risk of BC metastasis. A value of p < 0.05 was considered statistically significant. Results: According to the results, independent predictors of a high risk of BC regional metastasis were T2 (OR = 5.81, 95% CI = 1.75–19.35, p = 0.004) and T3-4 (OR = 43.07, 95% CI = 9.31–199.2, p < 0.0001) stages of BC, absence of an intraductal component (OR = 3.68, 95% CI = 1.32–10.33, p = 0.013), presence of lymphovascular invasion (OR = 3.32, 95% CI = 1.34–8.22, p = 0.009), luminal B HER2-positive (OR = 6.82, 95% CI = 1.13–42.26, p = 0.036) and triple negative (OR = 8.52, 95% CI = 1.12–64.89, p = 0.038) molecular biological subtypes of BC, and PD-L1 expression coefficient greater than 1.65 (OR = 6.39, 95% CI = 2.54–16.09, p = 0.0001). Based on the data obtained, an original noninvasive method for assessing a high risk of BC regional metastasis was developed, the sensitivity of which was 80.9%, the specificity - 82.6%, and the accuracy - 85.7%. The area under the curve (AUC) was 0.876 (95% CI = 0.818–0.924, р < 0.0001). Conclusion: The results of the study indicate that the assessment of PD-L1 expression in a tumor can improve the accuracy of predicting the risk of regional BC metastasis.

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Section: Immunohistochemical Studymentioning

confidence: 99%

Assessment of PD-L 1 expression in a tumor improves the accuracy of predicting the risk of regional breast cancer metastases

Zubareva,

Senchukova,

Shubin

et al. 2023

Trends Immunother.

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“…В ранее опубликованном исследовании мы также изучали экспрессию PD-L1 в паренхиме опухоли и иммунных клетках стромы при РМЖ [16]. В отличие от вышеперечисленных авторов, мы оценивали не частоту экспрессии PD-L1, а ее выраженность, которая учитывала долю экспрессирующих клеток и интенсивность окраски маркера.…”

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The features of PD-L1 expression in tumor stromal cells, peritumoral microvessels and isolated clusters of tumor cells in breast cancer tissue and their correlation with clinical and morphological characteristics of breast cancer

Zubareva,

Senchukova,

Karmakova

et al. 2023

Sib. onkol. ž.

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Objective: to study the features of PD-L1 expression in tumor stromal cells, peritumoral microvessels, and isolated clusters of tumor cells in breast cancer (Bc) tissue and their correlation with the clinical and morphological characteristics of Bc.Material and Methods. The study included 158 patients with newly diagnosed invasive BC. PD-L1 expression was studied by immunohistochemistry. statistical analysis was performed using statistica 12.0 software.Results. PD-L1 expression in peritumoral microvessels occurred in 41.4 and 61.7 % of cases with t1–2 and T3–4 (p=0.020), and in 39.8 and 51.7 % of cases with N0–1 and N2–3 (p=0.008), respectively. In isolated clusters of tumor cells, the marker expression was observed in 28.0 and 52.5 % of cases in nodular and diffuse forms of BC (p=0.005); in 25.9, 39.3 and 66.7 % of cases at stages I–IIb, IIIa–IIIc and IV (p=0.011); in 30.3, 26.2, 40.0 and 52.5 % of cases in T1, T2, T3 and T4 (p=0.040); and in 28.2 and 45.5 % of cases in N0–1 and N2–3 (p=0.030), respectively. Nuclear expression of PD-L1 was also detected in stromal cells, and was observed in 28.8 and 55.0 % of cases with nodular and diffuse forms of BC (p=0.003), in 17.6, 52.5 and 75.0 % of cases in early, locally advanced and metastatic BC (p<0.001), in 21.2, 28.7, 80.0 and 55.0 % of cases in T1, T2, T3 and T4 (p=0.002), in 21.7, 35.3, 51.4 and 55.0 % of cases with N0, N1, N2 and N3 (p=0.005), in 49.0 and 29.0 % of cases with negative and positive status of PR (p=0.014), in 30.3 and 52.8 % of cases with HER2-negative and HER2-positive BC status (p=0.014), respectively.Conclusion. The data indicate the relationship between PD-L1 expression and BC progression. The determination of PD-L1 expression in peritumoral microvessels and isolated tumor cell clusters, as well as nuclear expression of the marker, can be used to clarify the prognosis of the disease.

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Predictive significance of HIF-1α, Snail, and PD-L1 expression in breast cancer

Cited by 2 publications

References 45 publications

Assessment of PD-L 1 expression in a tumor improves the accuracy of predicting the risk of regional breast cancer metastases

Assessment of PD-L 1 expression in a tumor improves the accuracy of predicting the risk of regional breast cancer metastases

The features of PD-L1 expression in tumor stromal cells, peritumoral microvessels and isolated clusters of tumor cells in breast cancer tissue and their correlation with clinical and morphological characteristics of breast cancer

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