Predictive toxicological effects of Artemisia absinthium essential oil on hepatic stellate cells

Barreto, I.I.; Gonçalves, L.R.; Corrêa, A.F.; Marin-Morales, M.A.; Moraes, K.C.M.

doi:10.1016/j.tiv.2023.105738

Toxicology in Vitro

2024

DOI: 10.1016/j.tiv.2023.105738

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Predictive toxicological effects of Artemisia absinthium essential oil on hepatic stellate cells

I.I. Barreto,

L.R. Gonçalves,

A.F. Corrêa

et al.

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2024

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Cited by 1 publication

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Inhibition of the miR-1914-5p increases the oxidative metabolism in cellular model of steatosis by modulating the Sirt1-PGC-1α pathway and systemic cellular activity

Porto-Barbosa,

Ramos,

Pansa

et al. 2024

PLoS ONE

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Metabolic associated fatty liver disease (MAFLD) is considered an indicator of metabolic syndrome, which affects millions of people around the world and no effective treatment is currently available. MAFLD involves a wide spectrum of liver damage, that initiates from steatosis (fatty live) and may progress to more complex pathophysiology. Then, details in lipid metabolism controlling should be explored aiming to control the fatty liver. In this context, the miR-1914-5p can be considered a potential biotechnology tool to control lipid metabolism in hepatic cells. This miRNA finds potential mRNA binding sequences in more than 100 molecules correlated with energy production and lipid metabolism pointed in bioinformatic platforms. The present study addressed the miR-1914-5p effects in hepatic HepG2/LX-2 co-cultured cells in a in vitro steatotic environment stablished by the addition of 400 μM of a mixture of oleic and palmitic acids. The analyses demonstrated that the inhibition of the miRNA reduced energetic metabolites such as total lipids, triglycerides, cholesterol and even glucose. In addition, the miR-inhibitor-transfected cells did not present any deleterious effect in cellular environment by controlling reactive oxygen species production (ROS), mitochondrial membrane potential (ΔΨm) and even the pro-inflammatory environment. Moreover, the functional effect of the investigated miR, suggested its close connection to the modulation of Sirt-1-PGC1-α pathway, a master switch metabolic route that controlls cellular energetic metabolism. Our assays also suggested a synergistic effect of this miR-1914-5p in cell metabolism, which should be considered as a strong candidate to control steatotic environment in future clinical trials.

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Inhibition of the miR-1914-5p increases the oxidative metabolism in cellular model of steatosis by modulating the Sirt1-PGC-1α pathway and systemic cellular activity

Porto-Barbosa,

Ramos,

Pansa

et al. 2024

PLoS ONE

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show abstract

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Predictive toxicological effects of Artemisia absinthium essential oil on hepatic stellate cells

Cited by 1 publication

References 55 publications

Inhibition of the miR-1914-5p increases the oxidative metabolism in cellular model of steatosis by modulating the Sirt1-PGC-1α pathway and systemic cellular activity

Inhibition of the miR-1914-5p increases the oxidative metabolism in cellular model of steatosis by modulating the Sirt1-PGC-1α pathway and systemic cellular activity

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