Predictive value of collagen in cancer

Angel, Peggi; Zambrzycki, Stephen

doi:10.1016/bs.acr.2022.02.004

Cited by 16 publications

(11 citation statements)

References 170 publications

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“…Most of these genes encode either for structural proteins (COL5A3), components of the extracellular matrix (THSD4) or contributing to protein folding and/or degradation (FBXL16, PDIA6, PPIB). They are involved in proliferation, EMT, adhesion, and migration processes [46][47][48][49][50] , thereby strengthening the observed association between MAPT expression and proliferation and EMT processes. Interestingly, the protein encoded by the best-associated gene, FBXL16, stabilizes C-MYC by blocking its ubiquitination and promotes cancer cell proliferation and migration 73 .…”

Section: Discussionsupporting

confidence: 53%

“…3b). Among the top genes, some encode for fundamental structural proteins such as a collagen subunit (COL5A3) or an extracellular matrix glycoprotein (THSD4), which play a weighty role in EMT and migration processes 46,47 . Other genes (e.g., FBXL16, PDIA6, PPIB) are involved in processes of protein folding and/or degradation, and their possible implication in tumorigenesis has been reported [48][49][50] (Figure S9).…”

Section: Mapt Co-expression Analysis Highlighted P53-dependent Associ...mentioning

confidence: 99%

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Cancer-specific association between Tau (MAPT) and cellular pathways, clinical outcome, and drug response

Callari,

Sola,

Magrin

et al. 2023

Sci Data

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Tau (MAPT) is a microtubule-associated protein causing common neurodegenerative diseases or rare inherited frontotemporal lobar degenerations. Emerging evidence for non-canonical functions of Tau in DNA repair and P53 regulation suggests its involvement in cancer. To bring new evidence for a relevant role of Tau in cancer, we carried out an in-silico pan-cancer analysis of MAPT transcriptomic profile in over 10000 clinical samples from 32 cancer types and over 1300 pre-clinical samples from 28 cancer types provided by the TCGA and the DEPMAP datasets respectively. MAPT expression associated with key cancer hallmarks including inflammation, proliferation, and epithelial to mesenchymal transition, showing cancer-specific patterns. In some cancer types, MAPT functional networks were affected by P53 mutational status. We identified new associations of MAPT with clinical outcomes and drug response in a context-specific manner. Overall, our findings indicate that the MAPT gene is a potential major player in multiple types of cancer. Importantly, the impact of Tau on cancer seems to be heavily influenced by the specific cellular environment.

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Section: Discussionsupporting

confidence: 53%

Section: Mapt Co-expression Analysis Highlighted P53-dependent Associ...mentioning

confidence: 99%

Cancer-specific association between Tau (MAPT) and cellular pathways, clinical outcome, and drug response

Callari,

Sola,

Magrin

et al. 2023

Sci Data

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“…Alterations in extracellular assembly are driven by mutations, propeptide splice variants, changes in ECM composition or post-translational modifications, differential crosslinking and MMP activity [30,[40][41][42][43]. Subsequently, fiber measurements have been used to predict disease progression and outcome [39,44,45]. The variations and scalability of the biology presented throughout collagen synthesis, secretion and modeling direct the cell and structure network of TME form and function.…”

Section: Extracellular Matrix Form Leads To Functionmentioning

confidence: 99%

Molecular analysis of the extracellular microenvironment: from form to function

Macdonald,

Mehta,

Drake

et al. 2024

FEBS Letters

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The extracellular matrix (ECM) proteome represents an important component of the tissue microenvironment that controls chemical flux and induces cell signaling through encoded structure. The analysis of the ECM represents an analytical challenge through high levels of post‐translational modifications, protease‐resistant structures, and crosslinked, insoluble proteins. This review provides a comprehensive overview of the analytical challenges involved in addressing the complexities of spatially profiling the extracellular matrix proteome. A synopsis of the process of synthesizing the ECM structure, detailing inherent chemical complexity, is included to present the scope of the analytical challenge. Current chromatographic and spatial techniques addressing these challenges are detailed. Capabilities for multimodal multiplexing with cellular populations are discussed with a perspective on developing a holistic view of disease processes that includes both the cellular and extracellular microenvironment.

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“…Such doubled production of Collagen I and IV in cells cultured in CK 1 scaffold for 14-days could be due to the peculiar rigidity of CK 1 that matches with tumorigenic pancreatic tissue. Additional analysis on collagen fibers orientation could be significant in predicting cancer (Angel and Zambrzycki, 2022). Tumor-ECM is more abundant, condensed, and stiffer than the ECM in the surrounding healthy tissue.…”

Section: Fabrication Of Bioscaffolds For In Vitro 3d Hpis Culturesmentioning

confidence: 99%

Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels

Marchini

Ciulla²,

Antonioli³

et al. 2023

Front. Bioeng. Biotechnol.

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Human pancreatic islets transplantation is an experimental therapeutic treatment for Type I Diabetes. Limited islets lifespan in culture remains the main drawback, due to the absence of native extracellular matrix as mechanical support after their enzymatic and mechanical isolation procedure. Extending the limited islets lifespan by creating a long-term in vitro culture remains a challenge. In this study, three biomimetic self-assembling peptides were proposed as potential candidates to recreate in vitro a pancreatic extracellular matrix, with the aim to mechanically and biologically support human pancreatic islets, by creating a three-dimensional culture system. The embedded human islets were analyzed for morphology and functionality in long-term cultures (14-and 28-days), by evaluating β-cells content, endocrine component, and extracellular matrix constituents. The three-dimensional support provided by HYDROSAP scaffold, and cultured into MIAMI medium, displayed a preserved islets functionality, a maintained rounded islets morphology and an invariable islets diameter up to 4 weeks, with results analogues to freshly-isolated islets. In vivo efficacy studies of the in vitro 3D cell culture system are ongoing; however, preliminary data suggest that human pancreatic islets pre-cultured for 2 weeks in HYDROSAP hydrogels and transplanted under subrenal capsule may restore normoglycemia in diabetic mice. Therefore, engineered self-assembling peptide scaffolds may provide a useful platform for long-term maintenance and preservation of functional human pancreatic islets in vitro.

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Predictive value of collagen in cancer

Cited by 16 publications

References 170 publications

Cancer-specific association between Tau (MAPT) and cellular pathways, clinical outcome, and drug response

Cancer-specific association between Tau (MAPT) and cellular pathways, clinical outcome, and drug response

Molecular analysis of the extracellular microenvironment: from form to function

Long-term cultures of human pancreatic islets in self-assembling peptides hydrogels

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