Predictive Value of HPV E6/E7 mRNA Detection on the Outcome of Cervical LSIL

Abstract

Objective. To assess the predictive worth of HPV E6/E7 mRNA detection in the outcome of the cervical low-grade squamous intraepithelial lesion (LSIL). From September 2017 to early September 2019, patients screened for high-risk HPV positive or abnormal cervical liquid-based cytology were retrospectively analyzed and diagnosed with LSIL by cervical biopsy were recruited. The independent influencing factors of the regression of LSIL lesions after follow-up were analyzed, and the outcome of LSIL was calculated. The results of the initial colposcopy in this study were CIN I, CIN II/P16-negative, CIN II/P16-positive, and CIN III. At the time of re-examination, LSIL patients had three outcomes: regression, persistence, and progression. In the two follow-ups, 330 patients were finally included, including 276 CIN I patients (group A) and 54 CIN II/P16-negative patients (group B). The positive rates of HPV E6/E7 mRNA in each group were 66.67% and 70.37% for A and B, respectively. The total positive rate of E6/E7 mRNA was 67.27%, and there was no significant difference between the two groups ( P > 0.05 ). After 1 year follow-up, whether HPV E6/E7 mRNA regressed or was negative was associated with the outcome of LSIL-related lesions ( P < 0.05 ). The regression or negative rate of HPV E6/E7 mRNA was 1.57 times higher than the progression rate of HPV E6/E7 mRNA-positive diagnosis of LSIL lesions. Univariate logistic regression analysis showed that age at first sexual intercourse, HPV E6/E7 mRNA results, and lesion type were statistically significant ( P < 0.05 ). Whether HPV E6/E7 mRNA was negative (OR = 2.420, P = 0.001 ) and age at first sexual intercourse ≥20 years (OR = 0.420, P = 0.002 ) were independent influencing factors associated with LSIL regression. Multivariate logistic analysis showed that age of first sexual intercourse ≥20 years (OR = 0.420, P = 0.002 ) and HPV E6/E7 mRNA-negative (OR = 2.420, P = 0.001 ) were independent factors associated with LSIL. HPV E6/E7 mRNA detection can be used for predicting the outcome of LSIL and has a good application value.

Detection of Human Papillomavirus DNA and E6/E7 mRNA from HPV Genotypes 16, 18, 31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso

“…and there was no significant difference between CIN1 and CIN2/P16 negative [33]. All of the above findings support the notion that the mRNA test for HR-HPV oncoproteins E6 and E7 could serve as a potential biomarker to facili-tate early diagnosis and, consequently, the early management of high-grade intraepithelial cervical lesions and cervical cancer.…”

“…The detection of HR-HPV was carried out using multiplex real-time PCR, employing the HPV Genotypes 14 Real-TMQuant kit from SACACE Biotechnologies ® in Italy. This kit allows for the identification of 14 high-risk HPV genotypes (HPV- 16,18,31,33,35,39,45,51,52,56,58,59, 66, and 68) by targeting the L1 region of the HR-HPV genome.…”

“…Each tube contained primers for the target regions of three or four types of HR-HPV and the human beta-globin gene, which served as an internal control to monitor the presence of cellular material in the sample. Specifically, for each sample, we had the following four tubes: PCR-mix-1 (16,18,31,IC); PCR-mix-1 (39, 45, 59, IC); PCR-mix-1 (33,35,56,68); PCR-mix-1 (51, 52, 58, 66).…”

“…HPV infection is the most common sexually transmitted infection (STI) worldwide, with more than half of all adults becoming infected during their lifetime [2] [3] [4] [5]. Based on their oncogenic potential in humans, HPVs are classified into two categories: those with high oncogenic risk (including types 16,18,31,33,35,39,45,51,52,56,58,59, 66, and 68), which are associated with cancers of the anogenital and oropharyngeal regions, and low-risk HPVs (such as types 6,11,26,40,42,53,54,55,61,62, 64, 67, 69, 70, 71, 72, 73, 81, and 89), responsible for benign lesions like condyloma acuminata and warts [5] [6].…”

Retracted: Predictive Value of HPV E6/E7 mRNA Detection on the Outcome of Cervical LSIL

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process: Discrepancies in scope Discrepancies in the description of the research reported Discrepancies between the availability of data and the research described Inappropriate citations Incoherent, meaningless and/or irrelevant content included in the article Peer-review manipulation …”