Predictive value of intracellular ATP level for cell viability after heating in malignant cells

Kitamura, Kazuki; Kuwano, Hiroyuki; Matsuda, Hikaru; Toh, Yasushi; Maehara, Yoshihiko; Sugimachi, Keizō

doi:10.3109/02656739309061482

Cited by 5 publications

(1 citation statement)

References 11 publications

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“…Hyperthermia has been described as an effective method to treat various types of malignant cells, both in vitro and in vivo [1] Traditional hyperthermia involves heating the tissue to at least 43 • C for one hour, while high intensity thermal therapies involve higher temperature for shorter times. Traditional hyperthermia often involves two distinct phases.…”

Section: Basic Hyperthermia Treatmentsmentioning

confidence: 99%

Using Microwave Energy to Treat Tumors

Ibrahim¹

2008

PIER B

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Abstract-Recently, hyperthermia has been investigated as an alternate therapy for the treatment of tumors. This paper explored the feasibility of preferential hyperthermia as a method of treating deep seated tumors. The overall goal of this research was to determine theoretically if preferential heating could be used to attain the desired thermal dose (DTD) for a two cm diameter tumor. The simulations in this work show that, when using a single rod insert, the model cannot provide enough energy for an entire 2 cm diameter tumor to receive the DTD. However, when using an enhanced design model with multiple (4) rods inserts, the DTD could be attained in a tumor up to 3.5 cm in diameter. This study involved using the model a spherical 2 cm tumor, assuming the tumor is located in deep tissue with a constant perfusion rate and no major blood vessels nearby. This tumor was placed in the center of a cube of healthy tissue. To achieve the preferential heating of the tumor, a rod insert was placed in the center of the tumor and microwave energy was applied to the insert (in the form of volumetric heating). The thermal modeling of this system was based on the Pennes Bioheat equation with a maximum temperature limitation of 80 • C. Additional enhanced design models were also examined. These models include 2 cm and 4 cm tumors with four rod inserts symmetrically placed about the tumor and a 4 cm tumor model using a single rod insert with antennae attached to insert to increase energy distribution to the tumor. The simulations show that only the enhanced design cases with four rods inserts can achieve the DTD for an entire 2 cm tumor. The main purpose of this research was to determine if a minimally invasive treatment system using one or more rod inserts could be used to preferentially heat (and attain the DTD) a 2 cm diameter (or larger) tumor. Achieving the DTD for a 2 cm or larger tumor was important because currently the maximum diameter tumor that can be treated via hyperthermia is approximately

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Section: Basic Hyperthermia Treatmentsmentioning

confidence: 99%

Using Microwave Energy to Treat Tumors

Ibrahim¹

2008

PIER B

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Application of the ATP-bioluminescence assay to thermosensitivity testing for head and neck cancer

Ohtsubo

Saitô

Tanaka

et al. 1996

Eur Arch Otorhinolaryngol

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We investigated the usefulness of the ATP assay as a thermosensitivity test in comparison with a colony-forming assay (CFA). The intracellular ATP levels in KB cells were markedly decreased after exposure to hyperthermia (43 degrees C for 1 h), reaching less than half that in the control cells in 12-18 h, and recovering gradually thereafter. The effect of hyperthermia assessed by the ATP assay closely correlated to that assessed by CFA, not only when KB cells were heated at 42, 43, and 44 degrees C but also in the conditions where L, KB and IMC-3 cell lines were heated at 43 degrees C. Findings showed that the ATP assay can be performed easily and more quickly than CFA for evaluating cell viability after heating. We also investigated heterogeneous responses to hyperthermia in cells from fresh surgical specimens of tumors. Thus, this rapid and reliable test was found to be useful for predicting the outcome of hyperthermia as a clinical treatment.

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