Predictive Value of Liver Slices for Metabolism and Toxicity in Vivo: Use of Acetaminophen as a Model Hepatotoxicant

Histomorphological changes and cytochrome P450 isoforms expression and activities in precision-cut liver slices from neonatal rats

“…In that examination we observed histopathological changes which in many cases became evident especially in the central part of the slices. Similar observations have been described also by other authors using comparable incubation conditions (Smith et al, 1985(Smith et al, , 1986Beamand et al, 1993;Miller et al, 1993;Gokhale et al, 1995;Olinga et al, 1997;Toutain et al, 1998;Hashemi et al, 1999;Evdokimova et al, 2001;Fisher et al, 2001). These histopathological changes are paralleled by a decline in the biochemical functions (Wright and Paine, 1992;Lake et al, 1993;Gokhale et al, 1997;Olinga et al, 1997;Kuhn et al, 1998;Müller et al, 1998;Lerche-Langrand and Toutain, 2000;Evdokimova et al, 2001;Gebhardt et al, 2003).…”

Translational Research in Pharmacology and Toxicology Using Precision-Cut Tissue Slices

“…Using PCTS, large interspecies differences between human and animals were found for the toxicity of several drugs, such as in the liver for paracetamol, 72,73 its congener N-acetyl-meta-aminophenol, 72 coumarin, 74 voriconazole, 53 and in the lung for acroleine, 75 to name just a few.…”

Evaluation of cultured, precision-cut rat liver slices as a model to study drug-induced liver apoptosis

“…Since their first use for in vitro investigations (Warburg, 1923), precision-cut liver slices have been increasingly used in both descriptive and mechanistic studies for xenobiotic and liver-specific toxicity (Lerche-Langrand and Toutain, 2000;Onderwater et al, 2004). The model has shown utility to address questions related to liver metabolic zonation, pluricellular interactions, and may be used to investigate zone-specific injury by histological analysis (Toutain et al, 1996;Miller et al, 1993;Olinga et al, 2001).…”