Predictive value of liver transaminases levels in abdominal trauma

Bilgiç, İsmail; Gelecek, Sibel; Akgün, Ali Emre; Özmen, Mehmet Mahir

doi:10.1016/j.ajem.2014.03.052

Cited by 25 publications

(26 citation statements)

References 15 publications

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“…Further studies are needed. In patients with abdominal trauma, abnormal hepatic transaminase and LDH levels are associated with liver injury (18). Herein, we found that laminarin reduced the level of LDH at all doses, thus, laminarin may protect against this type of injury.…”

Section: Discussionmentioning

confidence: 70%

Laminarin Promotes Immune Responses and Reduces Lactate Dehydrogenase but Increases Glutamic Pyruvic Transaminase in Normal Mice In Vivo

2018

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Abstract. Background mg treatment. Laminarin did not affect T-cell and B-cell proliferation. Laminarin increased the level of GPT and reduced that of LDH at all doses, indicating laminarin can protect against liver injury. Laminarin is worthy of investigation in future experiments on improving immune responses.It is well documented that the immune system involves sophisticated networks, and is not only responsible for protecting the host against diseases including foreign materials but also protects against toxic substances (1). Therefore, there has been focus on developing immune responses in order to protect against toxic substances entering the human body (2). In particular, many studies have paid attention to improving immune responses in humans from natural products. 523

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Section: Discussionmentioning

confidence: 70%

Laminarin Promotes Immune Responses and Reduces Lactate Dehydrogenase but Increases Glutamic Pyruvic Transaminase in Normal Mice In Vivo

2018

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“…Alanine aminotransferase (ALT) is an enzyme present in almost all cells with high expression in liver cells . It is considered one of the most liver‐specific enzymes and its serum level is one of the most important tests for evaluation of liver injury . In viral hepatitis and other forms of liver disease associated with hepatic necrosis, serum ALT is elevated even before the clinical signs and symptoms of the disease appear .…”

Section: Introductionmentioning

confidence: 99%

“…1 It is considered one of the most liverspecific enzymes 2 and its serum level is one of the most important tests for evaluation of liver injury. 3,4 In viral hepatitis and other forms of liver disease associated with hepatic necrosis, serum ALT is elevated even before the clinical signs and symptoms of the disease appear. 1 Lactate dehydrogenase (LDH) is commonly found elevated in cancerous tissues, 5 and its serum level is elevated in acute liver failure.…”

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confidence: 99%

Real‐time ALT and LDH activities determined in viable precision‐cut mouse liver slices using hyperpolarized [1‐¹³C]pyruvate—Implications for studies on biopsied liver tissues

et al. 2018

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Precision‐cut liver slices (PCLS) are widely used in liver research as they provide a liver model with all liver cell types in their natural architecture. The purpose of this study was to demonstrate the use of PCLS for hyperpolarized metabolic investigation in a mouse model, for potential future application in liver biopsy cores. Fresh normal liver was harvested from six mice. 500 μm PCLS were prepared and placed in a 10 mm NMR tube in an NMR spectrometer and perfused continuously. 31P spectra were acquired to evaluate the presence of adenosine triphosphate (ATP) and validate viability in all samples. Hyperpolarized [1‐13C]pyruvate was flushed into the NMR tube in the spectrometer. Consecutive 13C NMR spectra were acquired immediately after the injection using both non‐selective (five injections, two livers) and selective RF excitation (six injections, three livers). The 31P spectra showed the characteristic signals of ATP, confirming the viability of the PCLS for more than 2.5 h in the spectrometer. After each of the [1‐13C]pyruvate injections, both [1‐13C]lactate and [1‐13C]alanine signals were detected. Selective RF excitation aimed at both [1‐13C]lactate and [1‐13C]alanine enabled better visualization and quantification of the metabolic activity. Using this acquisition approach only the newly formed metabolites are observed upon excitation, and their intensities relative to those of hyperpolarized pyruvate enable quantification of metabolite production rates. This rate of lactate and alanine production appeared to be constant throughout the measurement time, with alanine production about 2.3 times higher than lactate. In summary, the viability of PCLS in an NMR spectrometer was demonstrated and hyperpolarized [1‐13C]pyruvate metabolism was recorded. This study opens up the possibility of evaluating alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) activities in human liver biopsies, while preserving the tissue architecture and viability. In healthy, well‐perfused liver slices the ratio of ALT to LDH activity is about 2.3.

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“…11 According to TIAN Z, et al, who studied role of elevated liver transaminase levels in the diagnosis of liver injury after blunt abdominal trauma, he suggested that in patients with blunt abdominal trauma, abnormal transaminase levels are associated with liver injury. 12 Patients with ALT > 57 U/l and AST >113 U/l are strongly associated with liver injury.…”

Section: Discussionmentioning

confidence: 99%

Role of elevated liver transaminase enzymes in diagnosis of liver injury in cases after abdominal trauma

et al. 2016

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Predictive value of liver transaminases levels in abdominal trauma

Cited by 25 publications

References 15 publications

Laminarin Promotes Immune Responses and Reduces Lactate Dehydrogenase but Increases Glutamic Pyruvic Transaminase in Normal Mice In Vivo

Laminarin Promotes Immune Responses and Reduces Lactate Dehydrogenase but Increases Glutamic Pyruvic Transaminase in Normal Mice In Vivo

Real‐time ALT and LDH activities determined in viable precision‐cut mouse liver slices using hyperpolarized [1‐¹³C]pyruvate—Implications for studies on biopsied liver tissues

Role of elevated liver transaminase enzymes in diagnosis of liver injury in cases after abdominal trauma

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Predictive value of liver transaminases levels in abdominal trauma

Cited by 25 publications

References 15 publications

Laminarin Promotes Immune Responses and Reduces Lactate Dehydrogenase but Increases Glutamic Pyruvic Transaminase in Normal Mice In Vivo

Laminarin Promotes Immune Responses and Reduces Lactate Dehydrogenase but Increases Glutamic Pyruvic Transaminase in Normal Mice In Vivo

Real‐time ALT and LDH activities determined in viable precision‐cut mouse liver slices using hyperpolarized [1‐13C]pyruvate—Implications for studies on biopsied liver tissues

Role of elevated liver transaminase enzymes in diagnosis of liver injury in cases after abdominal trauma

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Real‐time ALT and LDH activities determined in viable precision‐cut mouse liver slices using hyperpolarized [1‐¹³C]pyruvate—Implications for studies on biopsied liver tissues