Predominance of Fourth Panzootic Newcastle Disease Virus Subgenotype VII.1.1 in Iran and Its Relation to the Genotypes Circulating in the Region

Molouki, Aidin; Sotani, Mohammad; Mehrabadi, Mohammad Hossein Fallah; Shoushtari, A; Abtin, Alireza; Akhijahani, Mohsen Mahmoudzadeh; Abdoshah, Mohammad; Pourbakhsh, Seyed Ali; Allahyari, Esameel; Ghalyanchilangeroudi, Arash; Engelsma, Marc; Lim, Swee Hua Erin

doi:10.1007/s00284-021-02572-z

Cited by 7 publications

(9 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sabouri et al (2018) firstly reported that this genotype was identified in backyard poultry in Iran during 2011-2013. It was later detected in many commercial broiler farms from 2017 to 2020 Allahyari et al, 2020;Molouki et al, 2021a). This is similar to our findings where most of the cases were isolated from commercial broilers.…”

Section: Discussionsupporting

confidence: 91%

“…Areas affected by ND outbreaks are where most of the commercial poultry farms in Sabah are located (Anon, 2019). In Iran, this sub-genotype was also detected in other avian species such as pigeons, magpie, pheasant, turkey, peacock and ostrich (Khosravi et al, 2021;Molouki et al, 2021aMolouki et al, , 2021b. Since then, this sub-genotype is considered as the predominant sub-genotype in Iran (Khosravi et al, 2021;Molouki et al, 2021b).…”

Section: Discussionmentioning

confidence: 99%

“…It has been found in commercial broiler farms as well as backyard poultry . This sub-genotype was previously thought of as subgenotype VIId due to different genotyping systems used in various studies (Sabouri et al, 2018;Molouki et al, 2021a). Sabouri et al (2018) firstly reported that this genotype was identified in backyard poultry in Iran during 2011-2013.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Newcastle Disease Virus sub-genotype VII 1.1 isolated from chickens in Sabah, Malaysia

et al. 2022

View full text Add to dashboard Cite

Newcastle disease (ND) is an extremely contagious and fatal viral disease causing huge economic losses to the poultry industry. Following recent ND outbreaks in Sabah in commercial poultry and backyard farms, it was speculated that this could be due to a new introduction of Newcastle Disease Virus (NDV) genotype/sub-genotype. Here we report the genetic characterization of NDVs isolated from Sabah during early 2021. All isolates were amplified and sequenced with primers specific to the viral fusion (F) gene using reverse transcription-polymerase chain reaction (RT-PCR). Nucleotide sequence analysis of the F gene showed that all isolates shared similar homology of 99.4% with NDV strain from Iran isolated in 2018. Amino acid sequences of the F protein cleavage site revealed the motif of 112 RRQKRF 117 indicating all isolates were of virulent strain. Phylogenetic analysis demonstrated that all isolates were clustered under sub-genotype VII 1.1 and clustered together with isolates from Iran (previously known as subgenotype VIIl). The present findings suggested that there is an emerging of a new sub-genotype into the poultry population in Sabah and this sub-genotype has never been reported before in Malaysia. Therefore, transboundary monitoring and continuous surveillance should be implemented for proper control and prevention of the disease. A further molecular epidemiological analysis of NDV is needed to well understand the circulatory patterns of virulent strains of NDV in the country to prevent future outbreaks.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Newcastle Disease Virus sub-genotype VII 1.1 isolated from chickens in Sabah, Malaysia

et al. 2022

View full text Add to dashboard Cite

show abstract

“…These findings suggest that this genotype remains endemic and circulating in many countries. Lately, genotype VII.1.1 of virulent strains NDV has been causing severe diseases outbreaks in poultry in several countries [82][83][84][85]. Dimitrov et al [3] reported that a single genotype (VII.1.1) was responsible for the fourth NDV panzootic.…”

Section: Discussionmentioning

confidence: 99%

Newcastle Disease Genotype VII Outbreaks in Poultry across Egypt are associated with Enzootic Prevalence of the Virus in Wild Birds

Eid¹,

Hussein²,

Hassanin³

et al. 2022

Preprint

View full text Add to dashboard Cite

Newcastle disease virus (NDV) genotype VII is a highly pathogenic Orthoavulavirus that has caused multiple outbreaks among poultry in Egypt since 2011. This study aimed to investigate the genetic diversity of NDV prevailing in domestic and wild birds in Egyptian governorates. A total of 37 oropharyngeal swabs from wild birds and 101 swabs from domestic bird flocks including chickens, ducks, turkeys, and swans were collected from different geographic regions within 13 governorates during 2019-2020. Virus isolation and propagation via embryonated eggs revealed 91 swab samples produced allantoic fluid containing hemagglutination activity, suggestive of virus presence. The use of RT-PCR targeted to F gene successfully detected NDV in 85 samples. The geographical prevalence of NDV spread to 12 governorates in domestic birds, migratory and non-migratory wild birds. Following whole genome sequencing, we assembled six NDV genome sequences (70 - 99% of genome coverage), including five full F gene sequences. All NDV strains carried high virulence, based on the presence of polybasic amino acids (RRQRF) at the F gene cleavage site. Phylogenetic analysis revealed that the NDV strains belonged to class II within genotype VII.1.1. The presence of genetically similar virulent NDV in wild birds further highlights their role in the dissemination of NDV in poultry populations across Egypt. Continued genomic surveillance in both wild birds and poultry would be necessary for monitoring NDV incursions and genetic diversification.

show abstract

“…Currently, ND is endemic in Iran and is one of the most important viral diseases that has affected the poultry industry despite the widespread use of mass vaccination. Studies in Iran during the last decade have shown that sub‐genotype VII.1.1 NDV is prevalent in the backyard and commercial broiler flocks (Allahyari et al., 2020 ; Molouki et al., 2019 , 2021 ; Sabouri et al., 2018 ). With much less frequency than VII.1.1, viruses of sub‐genotypes VII.2 and XIII.1.2 have been detected in Iran by the older NDV classification system that had been formerly named VIIi and VIIb, respectively (Dimitrov et al., 2019 ; Ebrahimi et al., 2012 ; Ghalyanchilangeroudi et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

First detection and characterisation of sub‐genotype XIII.2.1 Newcastle disease virus isolated from backyard chickens in Iran

Hejazi

Tabatabaeizadeh

Toroghi

et al. 2022

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Background Newcastle disease (ND) is an economically significant poultry disease worldwide. During field surveillance for ND in 2010 in Iran, a backyard chicken flock showed clinical signs of ND with 100% mortality. Objectives We aimed to characterise genetically, biologically and epidemiologically an exotic virulent ND virus (NDV) detected in Iran. Methods After observing high mortality, dead birds were sampled and then disposed of by burial, and the chicken house was disinfected. Tissue samples were molecularly tested for NDV. The genetic homogeneity of the isolate RT30/2010 was tested by plaque assay, and then a large virus plaque was used for the second step of plaque purification. Fusion and matrix complete genes were sequenced and used for genotyping and epidemiological tracing. We tested biological pathotypes using mean death time (MDT) and intracerebral pathogenicity index (ICPI) assays. Results The isolate formed heterogeneous plaques in chicken embryo fibroblast cells. The second step of plaque purification produced homogeneous and large plaques. Phylogenetic analysis using both genes classified the virus into sub‐genotype XIII.2.1. Nucleic acid and amino acid identities of RT30/2010 fusion gene with the closest available isolate SPVC/Karachi/NDV/43 are 97.95% and 98.73%, respectively. Isolate has 112RRRKRF117 motif at the fusion cleavage site, and pathogenicity tests showed MDT of 56.4 h and ICPI of 1.85. Conclusions This study presents the first detection and characterisation of a velogenic NDV of sub‐genotype XIII.2.1 from Iran. Our follow‐up surveillance for ND shows that timely virus detection and carcass management have led to the cessation of virus transmission in Iran.

show abstract

Predominance of Fourth Panzootic Newcastle Disease Virus Subgenotype VII.1.1 in Iran and Its Relation to the Genotypes Circulating in the Region

Cited by 7 publications

References 43 publications

Identification of Newcastle Disease Virus sub-genotype VII 1.1 isolated from chickens in Sabah, Malaysia

Identification of Newcastle Disease Virus sub-genotype VII 1.1 isolated from chickens in Sabah, Malaysia

Newcastle Disease Genotype VII Outbreaks in Poultry across Egypt are associated with Enzootic Prevalence of the Virus in Wild Birds

First detection and characterisation of sub‐genotype XIII.2.1 Newcastle disease virus isolated from backyard chickens in Iran

Contact Info

Product

Resources

About