Predominance of <i>Streptococcus suis</i> ST1 and ST7 in human cases in China, and detection of a novel sequence type, ST658

Zhou, Yang; Dong, Xingxing; Li, Zhiwei; Zou, Geng; Lin, Li; Wang, Xiaohong; Chen, Huanchun; Gasser, Robin B.; Li, Jinquan

doi:10.1080/21505594.2016.1243193

Cited by 20 publications

(18 citation statements)

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“…Serotype 2, ST7 isolates were another predominant sequence type and responsible for the 1998 and 2005 Chinese epidemics, which caused a total of 240 human infections, 52 of whom dead [2]. Surveillance for S.suis has been ongoing since last outbreak in our country [3][4][5]. Here we report two cases of successfully cured human infection with ST7 SS2.…”

Section: Introductionmentioning

confidence: 87%

“…Comparing to genome of representative epidemic isolates (05ZYH33 and 98HAH33) [3], a high degree identity was noted in the genome organizations and sequences. Two sporadic ST7 isolates carried all virulence factors except agglutinin receptor.…”

Section: Cases Presentationmentioning

confidence: 96%

“…According to the MLST database (https://pubmlst.org/ ssuis) and previous geographic reviews [1,[3][4][5][6], we summarized and mapped the geographic distribution of S.suis causing human infection in China. The collected epidemiological data showed that S. suis cases occur mainly in South China.…”

Section: Cases Presentationmentioning

confidence: 99%

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Human infection caused by Streptococcus suis serotype 2 in China: report of two cases and epidemic distribution based on sequence type

et al. 2020

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Background: Streptococcus suis is a zoonotic pathogen that causes serious systemic infections in pigs and occupation-related infections in humans who contact with pigs or pork products. In China, it has caused two outbreaks of human infection and surveillance for S.suis has been ongoing since last time. Case presentation: Two cases of meningitis and sepsis caused by S. suis were reported in this study. Both patients work in relation to the pork trade, a risk factor for S. suis infection. The outcome was favorable after a prolonged ceftriaxone therapy but one patient was left with mild hearing loss. Two isolates were identified as sequencing type (ST) 7, S. suis serotype 2 (SS2), which is one the most prevalent and cause two outbreaks in China. Whole-genome sequencing (WGS) revealed that a high degree identity was noted in the genome organizations and sequences between two sporadic ST7 SS2 isolates in this study and representative epidemic virulent isolates. Major differences among them are two sporadic ST7 SS2 isolates lacked a virulence factor called agglutinin receptor and an 89 K pathogenicity island (PAI), which plays important role in the pathogenesis of streptococcal toxic shock syndrome (STSS). A summary about STs of human infection with S. suis in China was completed. The result showed ST1 and ST7 were still the major STs and several novel STs were successfully discovered in different provinces. Conclusions: Our results enhanced the understanding of the ability to cause life-threatening infections in humans and the distribution and evolution of the S. suis in China.

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Section: Introductionmentioning

confidence: 87%

Section: Cases Presentationmentioning

confidence: 96%

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Human infection caused by Streptococcus suis serotype 2 in China: report of two cases and epidemic distribution based on sequence type

et al. 2020

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“…In this study, three STs belonging to CC1 were used: ST1 predominates in many European and Asian countries while ST7 is considered to be responsible for two outbreaks in China (5). ST658 (LSM102) is a newfound virulent ST isolated from a Chinese patient who had handled and eaten pork before admission to hospital (44). We found immunization with 2015033 protected mice from the fatal challenge of these different STs in CC1; to our knowledge, this is the first live vaccine reported to provide STs-independent immunity against S. suis .…”

Section: Resultsmentioning

confidence: 99%

A capsular polysaccharide-expressing live vaccine suppresses streptococcal toxic shock-like syndrome and provides sequence type-independent protection during Streptococcus suis infection

Chang

et al. 2018

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12Streptococcus suis (S. suis) is an encapsulated zoonotic pathogen, which is responsible for 13 bacterial meningitis and streptococcal toxic shock-like syndrome (STSLS). Despite many 14 attempts to develop an effective vaccine, none is currently available. Here, a capsular 15 polysaccharide (CPS)-expressing attenuated mutant 2015033 was constructed by deleting 16 five virulence-associated factors (sly, scpA, ssnA, fhb, and ssads) in an outbreak S. suis strain 17 SC19. Genes mentioned above are associated with either innate immunity-evading or tissue 18 barrier-invading. Deletion of these genes did not impact the growth ability and CPS 19 generation of 2015033, and the mutant exhibited no hemolytic activity to erythrocytes and no 20 cytotoxicity to different epithelial or endothelial cells. In addition, 2015033 was more easily 21 2 eliminated by whole human blood in vitro and by mouse blood in vivo. In addition, 2015033 22 showed a diminished invasive ability in different mouse organs (brain, lung, and liver) and 23 avirulent properties in mice associated with weak inflammation-inducing ability. 24Immunization with 2015033 triggered T cell-dependent immunity and this immunity 25 suppressed STSLS during SC19 infection by inhibiting excessive proinflammatory responses. 26In addition, immunization with 2015033 successfully conferred sequence type 27 (STs)-independent protection to mice during heterogeneous infections (ST1, ST7, and ST658). 28This study presents the feasibility of the strategy of multi-gene deletion for the development 29 of promising live vaccines against invasive encapsulated pathogens. 30 IMPORTANCE 31 S. suis is a traditional zoonotic agent causing human meningitis and STSLS, which is also a 32 neglected emerging food-borne pathogen. Increasing antimicrobial resistance invokes 33 reduction of preventative use of antibiotics in livestock creating an urgent need for effective 34 vaccines. Given the expression of CPS is the basis for promising vaccines against 35 encapsulated pathogens, and in order to find an effective and economical strategy for 36 CPS-based vaccine development, multi-gene deletion was introduced into the design of a S. 37 suis vaccine for the first time. From our results, CPS-expressing attenuated mutant 2015033 38 exhibited diminished evasive ability against the innate immune system and reduced invasive 39 properties against different host barriers. To our knowledge, 2015033 is the first 40 STSLS-suppressing S. suis vaccine to provide STs-independent protection during 41 heterogeneous infections. 42Streptococcus suis (S. suis) is an important zoonotic pathogen with considerably high 44 phenotypic heterogeneity, which can be classified into 33 serotypes based on the antigenicity 45 of their capsular polysaccharides (CPS) (1) and 1003 sequence types (STs) according to the 46 sequence of seven house-keeping genes (cpn60, dpr, recA, aroA, thrA, gki, and mutS) (2). As 47 the most virulent serotype, S. suis serotype 2 (S. suis 2) has caused a wide variety of diseases 48 in pigs, incl...

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“…Among the 33 known serotypes, Streptococcus suis serotype 2 is considered to be the most prevalent and virulent. Although only sporadic cases of S. suis infection have been reported in humans (3), two outbreaks (Jiangsu in 1998 and Sichuan in 2005) initiated in China have raised considerable concerns among public health and food safety professionals in recent years (4). …”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Hypervirulent and Vaccine Candidate Streptococcus suis Strain SC19

et al. 2017

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Predominance of Streptococcus suis ST1 and ST7 in human cases in China, and detection of a novel sequence type, ST658

Cited by 20 publications

References 16 publications

Human infection caused by Streptococcus suis serotype 2 in China: report of two cases and epidemic distribution based on sequence type

Human infection caused by Streptococcus suis serotype 2 in China: report of two cases and epidemic distribution based on sequence type

A capsular polysaccharide-expressing live vaccine suppresses streptococcal toxic shock-like syndrome and provides sequence type-independent protection during Streptococcus suis infection

Draft Genome Sequence of Hypervirulent and Vaccine Candidate Streptococcus suis Strain SC19

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