Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography

Lucero, Diego; Zago, Valeria; López, Gustavo; Cacciagiú, Leonardo; López, Graciela; Wikinski, Regina; Nakajima, Katsuyuki; Schreier, Laura

doi:10.1016/j.clinbiochem.2011.12.013

Cited by 25 publications

(16 citation statements)

References 28 publications

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“…Within other lipoprotein subfractions, similar observations are made for intermediate subclasses and VLDL in response to physical activity and diet [13,32]. The improvement in VLDL levels, classically elevated in MetS [33], seems to be notable because of its contribution to endothelium damages [34]. The proatherogenic/antiatherogenic ratio ApoB/ApoA1 shows stronger links to MetS and may be a better risk discriminator than the single proatherogenic measurement (ApoB) [11].…”

Section: Discussionmentioning

confidence: 82%

Atherogenic subfractions of lipoproteins in the treatment of metabolic syndrome by physical activity and diet – the RESOLVE trial

Dutheil

Walther²,

Chapier

et al. 2014

Lipids Health Dis

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BackgroundWe aimed to comprehensively evaluate lipoprotein profile including lipid particle size following a lifestyle intervention in metabolic syndrome (MetS) volunteers and to assess the associations between lipoprotein subfractions and carotid-intima-media-thickness (CIMT) – a surrogate indicator of atherogenesis.Methods100 participants (50–70 years) from the RESOLVE trial, underwent a one-year follow-up beginning with a three-week residential program combining high exercise volume (15-20 h/week), restrictive diet (-500 kcal/day), and education. For baseline references, 40 aged-matched healthy controls were recruited. Independent associations between subfractions of lipoproteins and CIMT were evaluated using a generalized estimating equations model accounting for variation in correlations between repeated measures. The lipoprotein subfractions profile was assessed using Lipoprint® electrophoresis allowing to separate: the very low-density lipoprotein (VLDL) fraction, then the intermediate-density lipoprotein (IDL) C, B and A, the low-density lipoprotein (LDL) with subfractions 1 and 2 as large LDL and subfractions 3 to 7 as small dense LDL (sdLDL), and the high density lipoprotein (HDL) subfractions categorized into large, intermediate, and small HDL. Apolipoproteins A1 and B were also measured.Results78 participants completed the program. At baseline, apolipoproteins B/A1, VLDL, sdLDL and small HDL were higher in MetS than in healthy controls; IDL, LDL size, large and intermediate HDL were lower. Despite time-related regains during the follow-up, lipoprotein subfractions traditionally involved in cardiovascular risk, such as sdLDL, improved immediately after the residential program with values closest to those of healthy controls. CIMT improved throughout the lifestyle intervention. Using a generalized estimating equations model, none of the subfractions of lipoproteins nor apolipoproteins were linked to CIMT.ConclusionsLipoprotein subfractions traditionally involved in CVR, decreased after the 3-week residential program. During a 12 month follow-up, the time-related regains remained closer to the values of healthy controls than they were at baseline. CIMT improved throughout the lifestyle intervention. However, we failed to demonstrate a link between some lipoprotein subfractions and the atherogenicity directly measured from the wall thickness of arteries (CIMT). Further investigations are required to explore the atherogenicity of lipoprotein subfractions.Trial registrationNCT00917917

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Section: Discussionmentioning

confidence: 82%

Atherogenic subfractions of lipoproteins in the treatment of metabolic syndrome by physical activity and diet – the RESOLVE trial

Dutheil

Walther²,

Chapier

et al. 2014

Lipids Health Dis

View full text Add to dashboard Cite

show abstract

“…Serum BCAA concentrations associated with these analytes. In metabolic syndrome, there is an accumulation of large VLDL and postprandial remnants lipoproteins, which are known to have high atherogenic potential (18), while large HDL particles are less atherogenic than small HDL particles (9, 16, 19). On the basis of this knowledge, we selected from the metabolomics platform a set of variables typically indicating atherogenic metabolic profile related to insufficient oxidative lipid metabolism, and found strong associations with serum BCAA concentrations, in particular with isoleucine and leucine.…”

Section: Discussionmentioning

confidence: 99%

Branched-Chain Amino Acid Levels Are Related with Surrogates of Disturbed Lipid Metabolism among Older Men

et al. 2016

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Aims/hypothesisExisting studies suggest that decreased branched-chain amino acid (BCAA) catabolism and thus elevated levels in blood are associated with metabolic disturbances. Based on such information, we have developed a hypothesis how BCAA degradation mechanistically connects to tricarboxylic acid cycle, intramyocellular lipid storage, and oxidation, thus allowing more efficient mitochondrial energy production from lipids as well as providing better metabolic health. We analyzed whether data from aged Finnish men are in line with our mechanistic hypothesis linking BCAA catabolism and metabolic disturbances.MethodsOlder Finnish men enriched with individuals having been athletes in young adulthood (n = 593; mean age 72.6 ± 5.9 years) responded to questionnaires, participated in a clinical examination including assessment of body composition with bioimpedance and gave fasting blood samples for various analytes as well as participated in a 2-h 75 g oral glucose tolerance test. Metabolomics measurements from serum included BCAAs (isoleucine, leucine, and valine).ResultsOut of the 593 participants, 59 had previously known type 2 diabetes, further 67 had screen-detected type 2 diabetes, 127 impaired glucose tolerance, and 125 impaired fasting glucose, while 214 had normal glucose regulation and one had missing glucose tolerance information. There were group differences in all of the BCAA concentrations (p ≤ 0.005 for all BCAAs), such that those with normal glucose tolerance had the lowest and those with diabetes mellitus had the highest BCAA concentrations. All BCAA levels correlated positively with body fat percentage (r = 0.29–0.34, p < 0.0001 for all). Expected associations with high BCAA concentrations and unfavorable metabolic profile indicators from metabolomics analysis were found. Except for glucose concentrations, the associations were stronger with isoleucine and leucine than with valine.Conclusion/interpretationThe findings provided further support for our hypothesis by strengthening the idea that the efficiency of BCAA catabolism may be mechanistically involved in the regulation of fat oxidation, thus affecting the levels of metabolic disease risk factors.

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“…Small dense LDL particles have a lower affinity for the LDL receptor and have been shown to be cleared less rapidly from the circulation than large LDL particles. 21,22 Individuals with MetS are characterized by TG-enriched large VLDL particles 23 and elevated apoCIII concentrations. 4 VLDL-apoB 100 catabolism is delayed in MetS subjects partly through the inhibitory effect of apoCIII on lipoprotein lipase activity.…”

Section: Discussionmentioning

confidence: 99%

Effect of Mediterranean Diet With and Without Weight Loss on Apolipoprotein B ₁₀₀ Metabolism in Men With Metabolic Syndrome

Richard

Couture

Ooi

et al. 2014

ATVB

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Objective— To assess the effect of a Mediterranean diet (MedDiet) with and without weight loss (WL) on apolipoprotein B 100 (apoB 100 ) metabolism in men with metabolic syndrome. Approach and Results— The diet of 19 men with metabolic syndrome (age, 24–62 years) was first standardized to a North American isoenergetic control diet for 5 weeks, followed by an isoenergetic MedDiet for an additional 5 weeks under full-feeding conditions (MedDiet−WL). Participants next underwent a 20-week supervised WL program under free-living conditions (−10.2±2.9% body weight; P <0.01) and finally consumed the MedDiet (5 weeks) under weight-stabilizing feeding conditions (MedDiet+WL). In vivo kinetic of apoB 100 was assessed in the fasted state at the end of the 3 controlled diets using a bolus of D 3 -leucine. Compared with the control diet, MedDiet−WL reduced low-density lipoprotein (LDL)-apoB 100 pool size (−14.2%, P <0.01) primarily through an increase in LDL-apoB 100 fractional catabolic rate (+30.4%, P =0.02) and increased LDL particle size ( P <0.01) but had no effect on very-LDL (VLDL)-apoB 100 pool size or triglyceride concentrations, despite a significant increase in VLDL-apoB 100 fractional catabolic rate (+25.6%; P =0.03). MedDiet+WL had no further effect on LDL-apoB 100 pool size and fractional catabolic rate but further increased LDL particle size and reduced VLDL-apoB 100 pool size versus the control diet primarily through an increase in VLDL-apoB 100 fractional catabolic rate (+30.7%; P <0.01). Conclusions— Consumption of MedDiet increases LDL size and reduces LDL-apoB 100 concentrations primarily by increasing the catabolism of LDL even in the absence of WL in men with metabolic syndrome. MedDiet seems to have a trivial effect on VLDL concentrations and kinetics unless accompanied by significant WL. Clinical Trial Registration —URL: http://www.clinicaltrials.gov . Unique identifier: NCT00988650.

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Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography

Cited by 25 publications

References 28 publications

Atherogenic subfractions of lipoproteins in the treatment of metabolic syndrome by physical activity and diet – the RESOLVE trial

Atherogenic subfractions of lipoproteins in the treatment of metabolic syndrome by physical activity and diet – the RESOLVE trial

Branched-Chain Amino Acid Levels Are Related with Surrogates of Disturbed Lipid Metabolism among Older Men

Effect of Mediterranean Diet With and Without Weight Loss on Apolipoprotein B ₁₀₀ Metabolism in Men With Metabolic Syndrome

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Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography

Cited by 25 publications

References 28 publications

Atherogenic subfractions of lipoproteins in the treatment of metabolic syndrome by physical activity and diet – the RESOLVE trial

Atherogenic subfractions of lipoproteins in the treatment of metabolic syndrome by physical activity and diet – the RESOLVE trial

Branched-Chain Amino Acid Levels Are Related with Surrogates of Disturbed Lipid Metabolism among Older Men

Effect of Mediterranean Diet With and Without Weight Loss on Apolipoprotein B 100 Metabolism in Men With Metabolic Syndrome

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Effect of Mediterranean Diet With and Without Weight Loss on Apolipoprotein B ₁₀₀ Metabolism in Men With Metabolic Syndrome