Predominance of novel DS-1-like G8P[8] rotavirus reassortant strains in children hospitalized with acute gastroenteritis in Thailand

Chan-it, Wisoot; Chanta, Chulapong; Ushijima, Hiroshi

doi:10.22541/au.167451365.57771803/v1

Cited by 2 publications

(3 citation statements)

References 25 publications

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“…Therefore, the bovine-like G8P[8] strains with the DS-1-like backbone detected in Midwestern and Southeastern Brazil in 2017 may have been introduced into the country from distinct pools of co-circulating bovine-like G8P[8] strains with the DS-1-like backbone. Intra-genotypic variability and distinct genotypic lineage constellations of the bovine-like G8P[8] strains with the DS-1-like backbone have been previously reported [ 78 , 79 , 80 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic Constellation of Human Rotavirus G8 Strains in Brazil over a 13-Year Period: Detection of the Novel Bovine-like G8P[8] Strains with the DS-1-like Backbone

Medeiros

França

Viana

et al. 2023

Viruses

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Rotavirus (RVA) G8 is frequently detected in animals, but only occasionally in humans. G8 strains, however, are frequently documented in nations in Africa. Recently, an increase in G8 detection was observed outside Africa. The aims of the study were to monitor G8 infections in the Brazilian human population between 2007 and 2020, undertake the full-genotype characterization of the four G8P[4], six G8P[6] and two G8P[8] RVA strains and conduct phylogenetic analysis in order to understand their genetic diversity and evolution. A total of 12,978 specimens were screened for RVA using ELISA, PAGE, RT-PCR and Sanger sequencing. G8 genotype represented 0.6% (15/2434) of the entirely RVA-positive samples. G8P[4] comprised 33.3% (5/15), G8P[6] 46.7% (7/15) and G8P[8] 20% (3/15). All G8 strains showed a short RNA pattern. All twelve selected G8 strains displayed a DS-1-like genetic backbone. The whole-genotype analysis on a DS-1-like backbone identified four different genotype-linage constellations. According to VP7 analysis, the Brazilian G8P[8] strains with the DS-1-like backbone strains were derived from cattle and clustered with newly DS-1-like G1/G3/G9/G8P[8] strains and G2P[4] strains. Brazilian IAL-R193/2017/G8P[8] belonged to a VP1/R2.XI lineage and were grouped with bovine-like G8P[8] strains with the DS-1-like backbone strains detected in Asia. Otherwise, the Brazilian IAL-R558/2017/G8P[8] possess a “Distinct” VP1/R2 lineage never previously described and grouped apart from any of the DS-1-like reference strains. Collectively, our findings suggest that the Brazilian bovine-like G8P[8] strains with the DS-1-like backbone strains are continuously evolving and likely reassorting with local RVA strains rather than directly relating to imports from Asia. The Brazilian G8P[6]-DS-1-like strains have been reassorted with nearby co-circulating American strains of the same DS-1 genotype constellation. However, phylogenetic analyses revealed that these strains have some genetic origin from Africa. Finally, rather than being African-born, Brazilian G8P[4]-DS-1-like strains were likely imported from Europe. None of the Brazilian G8 strains examined here exhibited signs of recent zoonotic reassortment. G8 strains continued to be found in Brazil according to their intermittent and localized pattern, thus, does not suggest that a potential emergence is taking place in the country. Our research demonstrates the diversity of G8 RVA strains in Brazil and adds to the understanding of G8P[4]/P[6]/P[8] RVA genetic diversity and evolution on a global scale.

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Section: Discussionmentioning

confidence: 99%

Genomic Constellation of Human Rotavirus G8 Strains in Brazil over a 13-Year Period: Detection of the Novel Bovine-like G8P[8] Strains with the DS-1-like Backbone

Medeiros

França

Viana

et al. 2023

Viruses

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“…The RVA vaccines have been introduced to these countries for at least 8 years. [16][17][18] The emergence and predominance of G8P [8] may be related to the introduction of rotavirus vaccines. [15][16][17][18][19][20][21] However, the impact of the COVID-19 pandemic cannot be ignored.…”

Section: As Shown Inmentioning

confidence: 99%

“…[16][17][18] The emergence and predominance of G8P [8] may be related to the introduction of rotavirus vaccines. [15][16][17][18][19][20][21] However, the impact of the COVID-19 pandemic cannot be ignored. Some nonpharmaceutical interventions against COVID-19, such as case…”

Section: As Shown Inmentioning

confidence: 99%

Major changes in prevalence and genotypes of rotavirus diarrhea in Beijing, China after RV5 rotavirus vaccine introduction

Tian,

Shen,

et al. 2024

Journal of Medical Virology

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To analyze the epidemiological characteristics of group A rotavirus (RVA) diarrhea in Beijing between 2019 and 2022 and evaluate the effectiveness of the RV5 vaccine. Stool specimens were collected from patients with acute diarrhea, and RVA was detected and genotyped. The whole genome of RVA was sequenced by fragment amplification and Sanger sequencing. Phylogenetic trees were constructed using Bayesian and maximum likelihood methods. Descriptive epidemiological methods were used to analyze the characteristics of RVA diarrhea. Test‐negative design was used to evaluate the vaccine effectiveness (VE) of the RV5. Compared with 2011–2018, RVA‐positive rates in patients with acute diarrhea under 5 years of age and adults decreased significantly between 2019 and 2022, to 9.45% (249/634) and 3.66% (220/6016), respectively. The predominant genotype of RVA had changed from G9‐VIP[8]‐III between 2019 and 2021 to G8‐VP[8]‐III in 2022, and P[8] sequences from G8‐VP[8]‐III strains formed a new branch called P[8]‐IIIb. The complete genotype of G8‐VP[8]‐III was G8‐P[8]‐I2‐R2‐C2‐M2‐A2‐N2‐T2‐E2‐H2. The VE of 3 doses of RV5 was 90.4% (95% CI: 28.8%–98.7%) against RVA diarrhea. The prevalence of RVA decreased in Beijing between 2019 and 2022, and the predominant genotype changed to G8P[8], which may be related to RV5 vaccination. Continuous surveillance is necessary to evaluate vaccine effectiveness and improve vaccine design.

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Predominance of novel DS-1-like G8P[8] rotavirus reassortant strains in children hospitalized with acute gastroenteritis in Thailand

Cited by 2 publications

References 25 publications

Genomic Constellation of Human Rotavirus G8 Strains in Brazil over a 13-Year Period: Detection of the Novel Bovine-like G8P[8] Strains with the DS-1-like Backbone

Genomic Constellation of Human Rotavirus G8 Strains in Brazil over a 13-Year Period: Detection of the Novel Bovine-like G8P[8] Strains with the DS-1-like Backbone

Major changes in prevalence and genotypes of rotavirus diarrhea in Beijing, China after RV5 rotavirus vaccine introduction

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